Protein Extraction Reagents
Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.
Pepsin Reagent, Ready To Use, Electron Microscopy Science
Supplier: Electron Microscopy Sciences
Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.
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Biotin Resin for Isolation of Avidin, Streptavidin & Neutravidin Coupled Molecules, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' Immobilized Biotin Resin is designed for the high affinity chromatography purifications of avidin, streptavidin and Neutravidin protein.
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Lightning in a Bottle™, Chemiluminescent Membrane/Blotting Peroxidase Substrate
Supplier: Immuno4 LLC
Developed specifically as a ready to use reagent for Blotting applications requiring ultra sensitivity with exceptional signal to noise ratios
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ImmunoHistoZyme™, Ready-to-Use, Electron Microscopy Sciences
Supplier: Electron Microscopy Sciences
This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.
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Q-sep® QuEChERS Extraction Salts, Restek
Supplier: Restek
QuEChERS methods are fast, easy, and cost-effective, and Restek Q-sep® products make QuEChERS procedures even simpler.
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Protein Reducing Agents, G-Biosciences
Supplier: G-Biosciences
A water soluble, odorless, non-toxic and stable protein reductant, G-Biosciences' Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to ß-mercaptoethanol and DTT (dithiothreitol)
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Factor Xa Protease, New England Biolabs
Supplier: New England Biolabs (NEB)
Factor Xa cleaves after the arginine residue in its preferred cleavage site Ile-(Glu or Asp)-Gly-Arg
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Trypsin/Lys-C Mix, MS Grade, Promega
Supplier: Promega Corporation
Trypsin/Lys-C Mix, Mass Spec Grade, is a mixture of Trypsin Gold, Mass Spectrometry Grade, and rLys-C, Mass Spec Grade. The Trypsin/Lys-C Mix is designed to improve digestion of proteins or protein mixtures in solution.
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ProteaseMAX™ Surfactant, Trypsin Enhancer, Promega
Supplier: Promega Corporation
ProteaseMAX™ surfactant, trypsin enhancer enhances the enzymatic performance of trypsin, chymotrypsin and Lys-C in digestion reactions.
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Accessories for InGel™ Silver for Protein Digestion, G-Biosciences
Supplier: G-Biosciences
Suitable for 100 protein spots. provides optimal buffered conditions for in gel trypsin digestion of proteins.
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Lumi Super Tracker™ Soluble/ELISA Chemiluminescent Peroxidase Substrate, ImmunO4
Supplier: Immuno4 LLC
Developed specifically for ELISA and In-Vitro Diagnostic applications requiring ultra sensitivity with exceptional signal to noise ratios.
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Iminobiotin Resin for Isolation of Avidin, Streptavidin and Neutravidin Complexes, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose
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VersaLyse™ RBC Lysing Solution, Beckman Coulter
Supplier: Beckman Coulter
VersaLyse lysing solution is intended for the lysis of red blood cells in the preparation of biological samples for flow cytometry analysis.
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Pierce™ Endoproteinase Asp-N Protease, MS Grade, Thermo Scientific
Supplier: Invitrogen
Asp-N Protease, MS Grade, is a highly specific endoproteinase used to improve sequence coverage in mass spectrometry protein identification applications. Store at -20˚C in a nonfrost-free freezer.
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Pierce™ Endoproteinase Chymotrypsin Protease (TLCK treated), MS Grade, Thermo Scientific
Supplier: Invitrogen
Chymotrypsin Protease, MS Grade, is a purified native endoproteinase validated for maximum activity and selectivity in proteomic and used in in-solution digestions and in-gel digestion of proteins from from 1-D or 2-D gels. Store at -20˚C in a nonfrost-free freezer.
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Pierce™ Bacterial Protein Extraction Reagents, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific B-PER Bacterial Protein Extraction Reagents are designed to extract soluble protein from bacterial cells without harsh chemicals or mechanical disruption.