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43 results for Protein Extraction Reagents

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Protein Extraction Reagents

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.

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Tissue Protein Extraction Reagent, Abcam

Supplier: ABCAM INC CA

This product is manufactured by BioVision, an Abcam company and was previously called 8002 EZLys™ Tissue Protein Extraction Reagent.

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Lysing Matrix Z, MP Biomedicals

Lysing Matrix Z, MP Biomedicals

Supplier: MP Biomedicals

Lysing Matrix Z is used for breaking tough plant and animal samples.

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Trypsin Antigen Retrieval Kit, Abcam

Supplier: ABCAM INC CA

Trypsin Antigen Retrieval Kit ab259256 is used for enzyme-based antigen retrieval.

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CHO/K1 Whole Cell Lysate, Rockland Immunochemical

Supplier: Rockland Immunochemical

Ready-to-use whole cell lysates are derived from cell lines or tissues using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility.

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Pierce™ Trypsin Protease, MS Grade, Thermo Scientific

Pierce™ Trypsin Protease, MS Grade, Thermo Scientific

Supplier: Invitrogen

Pierce™ Trypsin Protease, MS Grade, is highly purified porcine trypsin that has been chemically modified for maximum activity and stability in proteomic applications.

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Trypsin Antigen Retrieval Solution, Abcam

Supplier: ABCAM INC CA

Trypsin-based antigen retrieval solution for Protease-Induced Epitope Retrieval (PIER) (ab970).

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FLUOR DE LYS® Developer II Concentrate, Enzo Life Sciences

FLUOR DE LYS® Developer II Concentrate, Enzo Life Sciences

Supplier: Enzo Life Sciences

FLUOR DE LYS® Developer II is an essential reagent for signal generation in assays done with the FLUOR DE LYS®-HDAC8, FLUOR DE LYS®-SIRT1 and FLUOR DE LYS®-SIRT2 substrates.

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Pierce™ Detergent Removal Spin Column, Thermo Scientific

Pierce™ Detergent Removal Spin Column, Thermo Scientific

Supplier: Invitrogen

Pierce™ Detergent Removal Resin efficiently binds to and removes high concentrations of detergents from 10 µL to 1 mL samples with minimal sample loss.

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FASP Protein Digestion Kit (FFPE), Abcam

Supplier: ABCAM INC CA

FASP Protein Digestion Kit (FFPE) (ab270543) allows unbiased extraction, complete proteome solubilization, and highly efficient digestion of FFPE tissue.

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Pierce™ Bacterial Protein Extraction Reagents, Thermo Scientific

Pierce™ Bacterial Protein Extraction Reagents, Thermo Scientific

Supplier: Invitrogen

Thermo Scientific B-PER Bacterial Protein Extraction Reagents are designed to extract soluble protein from bacterial cells without harsh chemicals or mechanical disruption.

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SMART Digest™ Protein Digests Kits

SMART Digest™ Protein Digests Kits

Supplier: Thermo Scientific

Obtain high quality analytical results from protein digests by using Thermo Scientific™ SMART Digest™ Kits.

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Universal HIER antigen retrieval reagent (10X), Abcam

Supplier: ABCAM INC CA

Universal HIER antigen retrieval reagent (10X) is a heat-induced epitope retrieval (HIER) solution that is compatible with virtually all antibodies and eliminates the need for multiple buffers, including citrate buffer, EDTA or high pH Tris buffers. - Cited in over 20 publications - Colour-coded pH indicator for accuracy - Superior pH stability at high temperatures - Non-toxic, odorless, and azide-free - Optimized for FFPE tissue antigen retrieval

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HistoReveal (15ml), Abcam

Supplier: ABCAM INC CA

Proteolytic antigen retrieval (PIER) reagent HistoReveal (ab103720).

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FLUOR DE LYS® Developer I Concentrate, Enzo Life Sciences

FLUOR DE LYS® Developer I Concentrate, Enzo Life Sciences

Supplier: Enzo Life Sciences

FLUOR DE LYS® Developer is an essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate. An appropriate FLUOR DE LYS® family substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.).

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NP-40 Alternative, MilliporeSigma

Supplier: MilliporeSigma

NP-40 alternative is a non-ionic surfactant used in the isolation and purification of functional membrane protein complexes and in two-dimensional (2D) gel electrophoresis.

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PROTEIN GRADE® Detergent, C12E9, Calbiochem®, MilliporeSigma

Supplier: MilliporeSigma

A non-ionic detergent used for the solubilization of membrane-bound proteins in their native state.

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Lumi Super Tracker™ Membrane/Blotting Chemiluminescent Peroxidase Substrate, ImmunO4

Supplier: Immuno4 LLC

Developed specifically for membrane/blotting applications requiring ultra sensitivity with exceptional signal to noise ratios.

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B-PER™ Pierce™ Complete Bacterial Protein Extraction Reagent, Thermo Scientific

B-PER™ Pierce™ Complete Bacterial Protein Extraction Reagent, Thermo Scientific

Supplier: Invitrogen

B-PER™ Complete Bacterial Protein Extraction Reagent is an all-in-one formulation of non ionic detergent and enzymes that enables mild extraction of proteins from both gram-negative and gram-positive bacteria without the need for mechanical disruption. B-PER Complete Reagent combines a universal nuclease and lysozyme to improve cell lysis and reduce viscosity, and may be used for soluble protein extraction and inclusion body purification from bacterial cell lysates.

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Lightning in a Bottle™, Chemiluminescent Membrane/Blotting Peroxidase Substrate

Supplier: Immuno4 LLC

Developed specifically as a ready to use reagent for Blotting applications requiring ultra sensitivity with exceptional signal to noise ratios

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Biotin Resin for Isolation of Avidin, Streptavidin & Neutravidin Coupled Molecules, G-Biosciences

Supplier: G-Biosciences

G-Biosciences' Immobilized Biotin Resin is designed for the high affinity chromatography purifications of avidin, streptavidin and Neutravidin protein.

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Pepsin Reagent, Ready To Use, Electron Microscopy Science

Supplier: Electron Microscopy Sciences

Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.

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Pronase Reagent, Ready To Use, Electron Microscopy Science

Supplier: Electron Microscopy Sciences

Pronase is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.

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Q-sep® QuEChERS Extraction Salts, Restek

Q-sep® QuEChERS Extraction Salts, Restek

Supplier: Restek

QuEChERS methods are fast, easy, and cost-effective, and Restek Q-sep® products make QuEChERS procedures even simpler.

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ProteaseMAX™ Surfactant, Trypsin Enhancer, Promega

ProteaseMAX™ Surfactant, Trypsin Enhancer, Promega

Supplier: Promega Corporation

ProteaseMAX™ surfactant, trypsin enhancer enhances the enzymatic performance of trypsin, chymotrypsin and Lys-C in digestion reactions.

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Trypsin/Lys-C Mix, MS Grade, Promega

Trypsin/Lys-C Mix, MS Grade, Promega

Supplier: Promega Corporation

Trypsin/Lys-C Mix, Mass Spec Grade, is a mixture of Trypsin Gold, Mass Spectrometry Grade, and rLys-C, Mass Spec Grade. The Trypsin/Lys-C Mix is designed to improve digestion of proteins or protein mixtures in solution.

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Factor Xa Protease, New England Biolabs

Factor Xa Protease, New England Biolabs

Supplier: New England Biolabs (NEB)

Factor Xa cleaves after the arginine residue in its preferred cleavage site Ile-(Glu or Asp)-Gly-Arg

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Protein Reducing Agents, G-Biosciences

Protein Reducing Agents, G-Biosciences

Supplier: G-Biosciences

A water soluble, odorless, non-toxic and stable protein reductant, G-Biosciences' Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to ß-mercaptoethanol and DTT (dithiothreitol)

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Lumi Super Tracker™ Soluble/ELISA Chemiluminescent Peroxidase Substrate, ImmunO4

Supplier: Immuno4 LLC

Developed specifically for ELISA and In-Vitro Diagnostic applications requiring ultra sensitivity with exceptional signal to noise ratios.

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Iminobiotin Resin for Isolation of Avidin, Streptavidin and Neutravidin Complexes, G-Biosciences

Supplier: G-Biosciences

G-Biosciences' Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose

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ImmunoHistoZyme™, Ready-to-Use, Electron Microscopy Sciences

Supplier: Electron Microscopy Sciences

This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.

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