Protein Extraction Reagents
Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.
Pierce™ Trypsin Protease, MS Grade, Thermo Scientific
Supplier: Thermo Scientific
Pierce™ Trypsin Protease, MS Grade, is highly purified porcine trypsin that has been chemically modified for maximum activity and stability in proteomic applications.
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Tissue Protein Extraction Reagent, Abcam
Supplier: Abcam
This product is manufactured by BioVision, an Abcam company and was previously called 8002 EZLys™ Tissue Protein Extraction Reagent.
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Trypsin Antigen Retrieval Kit, Abcam
Supplier: Abcam
Trypsin Antigen Retrieval Kit ab259256 is used for enzyme-based antigen retrieval.
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FLUOR DE LYS® Developer II Concentrate, Enzo Life Sciences
Supplier: Enzo Life Sciences
FLUOR DE LYS® Developer II is an essential reagent for signal generation in assays done with the FLUOR DE LYS®-HDAC8, FLUOR DE LYS®-SIRT1 and FLUOR DE LYS®-SIRT2 substrates.
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Pierce™ Detergent Removal Spin Column, Thermo Scientific
Supplier: Thermo Scientific
Pierce™ Detergent Removal Resin efficiently binds to and removes high concentrations of detergents from 10 µL to 1 mL samples with minimal sample loss.
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Trypsin Antigen Retrieval Solution, Abcam
Supplier: Abcam
Trypsin-based antigen retrieval solution for Protease-Induced Epitope Retrieval (PIER) (ab970).
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FLUOR DE LYS® Developer I Concentrate, Enzo Life Sciences
Supplier: Enzo Life Sciences
FLUOR DE LYS® Developer is an essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate. An appropriate FLUOR DE LYS® family substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.).
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HistoReveal (15ml), Abcam
Supplier: Abcam
Proteolytic antigen retrieval (PIER) reagent HistoReveal (ab103720).
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FASP Protein Digestion Kit (FFPE), Abcam
Supplier: Abcam
FASP Protein Digestion Kit (FFPE) (ab270543) allows unbiased extraction, complete proteome solubilization, and highly efficient digestion of FFPE tissue.
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Lysing Matrix Z, MP Biomedicals
Supplier: MP Biomedicals
Lysing Matrix Z is used for breaking tough plant and animal samples.
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NP-40 Alternative, MilliporeSigma
Supplier: MilliporeSigma
NP-40 alternative is a non-ionic surfactant used in the isolation and purification of functional membrane protein complexes and in two-dimensional (2D) gel electrophoresis.
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Universal HIER antigen retrieval reagent (10X), Abcam
Supplier: Abcam
Universal HIER antigen retrieval reagent (10X) is a heat-induced epitope retrieval (HIER) solution that is compatible with virtually all antibodies and eliminates the need for multiple buffers, including citrate buffer, EDTA or high pH Tris buffers. - Cited in over 20 publications - Colour-coded pH indicator for accuracy - Superior pH stability at high temperatures - Non-toxic, odorless, and azide-free - Optimized for FFPE tissue antigen retrieval
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SMART Digest™ Protein Digests Kits
Supplier: Thermo Scientific
Obtain high quality analytical results from protein digests by using Thermo Scientific™ SMART Digest™ Kits.
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Lumi Super Tracker™ Membrane/Blotting Chemiluminescent Peroxidase Substrate, ImmunO4
Supplier: Immuno4 LLC
Developed specifically for membrane/blotting applications requiring ultra sensitivity with exceptional signal to noise ratios.
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CHO/K1 Whole Cell Lysate, Rockland Immunochemical
Supplier: Rockland Immunochemical
Ready-to-use whole cell lysates are derived from cell lines or tissues using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility.
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Pepsin Reagent, Ready To Use, Electron Microscopy Science
Supplier: Electron Microscopy Sciences
Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.
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