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You searched for: Protein Purification Reagents

Protein Purification Reagents

Protein purification reagents are for use in isolating one or more proteins from a complex mixture, usually cells, tissues, or whole organisms. This process is vital for function and structure characterization, as well as identifying interactions of the protein of interest. Important during the many steps, the protein purification reagents assist with cell lysis, separating the soluble protein components from cell debris, and finally separating the protein of interest from product- and process-related impurities.

J.T.Baker® BAKERBOND® Protein Precipitation Plates

J.T.Baker® BAKERBOND® Protein Precipitation Plates

Supplier: Avantor

J.T.Baker® BAKERBOND® protein precipitation plates enable fast protein removal from biological samples.

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J.T.Baker® BAKERBOND® Supported Liquid Extraction Plates

J.T.Baker® BAKERBOND® Supported Liquid Extraction Plates

Supplier: Avantor

J.T.Baker® BAKERBOND® supported liquid extraction (SLE) plates are an ideal and faster alternative to traditional liquid-liquid extraction methods and allow excellent recoveries of analytes from biological matrices.

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APO-12

Supplier: MilliporeSigma

Non-ionic detergent useful in two-phase separation and purification of proteins.

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GENAPOL®, Protein Grade® Detergent, Calbiochem

Supplier: MilliporeSigma

GENAPOL® Detergents are polyglycol ethers, purified for the isolation of membrane protein.

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Calbiochem®, Aquacide, MilliporeSigma

Supplier: MilliporeSigma

Aquacides are useful for concentration of proteins and other macromolecules and can be used for rapid removal of water from dialysis tubing by using either a 2% syrup or the aquacide solid. Aquacides neither denature proteins nor change the pH during concentration. At room temperature 1 g of aquacide absorbs about 5 ml of water from solution in 1 h.

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STRATEGY™ for Developing the Best Protein Purification Strategy, G-Biosciences

Supplier: G-Biosciences

G-Biosciences' STRATEGY™ consists of numerous fractionation and chromatography techniques to aid researchers to develop a suitable purification system for specific and novel proteins

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Super Shield™ Alkaline Phosphatase (AP) Conjugate and Protein Stabilizer, ImmunO4

Supplier: Immuno4 LLC

Preserves and stabilizes the activity of Alkaline Phosphatase (AP) conjugates and proteins used in immunoassays and molecular assays.

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Super Shield™ Peroxidase (HRP) Conjugate and Protein Stabilizer, ImmunO4

Supplier: Immuno4 LLC

Preserves and stabilizes the activity of HRP conjugates and proteins used in immunoassays and molecular assays.

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Super Shield™ Macro™ Universal Protein Stabilizer, ImmunO4

Supplier: Immuno4 LLC

Preserves and stabilizes the activity of various biomolecules used in immunoassays and molecular assays.

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Armor Plate™ Non-Mammalian Protein Coat Block and Diluent

Supplier: Immuno4 LLC

Blocks and stabilizes coated ELISA plates and other solid phase substrates.

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a1-3,4,6 Galactosidase, New England Biolabs

a1-3,4,6 Galactosidase, New England Biolabs

Supplier: New England Biolabs (NEB)

a1-3,4,6 Galactosidase is a highly specific exoglycosidase that catalyzes the hydrolysis of α1-3, α1-4 and α1-6 linked d-galactopyranosyl residues from oligosaccharides.

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Armor Plate™ Milk Protein Coat Block and Diluent, ImmunO4

Supplier: Immuno4 LLC

Blocks and stabilizes coated ELISA plates and other solid phase substrates.

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a1-3,4 Fucosidase, New England Biolabs

a1-3,4 Fucosidase, New England Biolabs

Supplier: New England Biolabs (NEB)

α1-3,4 Fucosidase, (also known as AMF) is a broad specificity exoglycosidase that catalyzes the hydrolysis of α1-3 and α1-4 linked fucose residues from oligosaccharides and glycoproteins.

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Super Shield™ Symulator™ Peroxidase (HRP) Conjugate and Protein Stabilizer, ImmunO4

Supplier: Immuno4 LLC

Preserves and stabilizes the activity of Peroxidase (HRP) conjugates and proteins used in immunoassays and molecular assays.

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Protein Concentrator Solution, G-Biosciences

Supplier: G-Biosciences

Concentrator Solution is a novel liquid polymer for the rapid concentration of dilute protein solutions with zero loss, using dialysis

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Protein Concentrator Powder, G-Biosciences

Protein Concentrator Powder, G-Biosciences

Supplier: G-Biosciences

Concentrator Powder is a high molecular weight polymer which will not migrate across the dialysis membrane

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Protein Deglycosylation Mix II, New England Biolabs

Protein Deglycosylation Mix II, New England Biolabs

Supplier: New England Biolabs (NEB)

The Protein Deglycosylation Mix II contains all of the enzymes, reagents, and controls needed to remove all N-linked and simple O-linked glycans as well as some complex O-linked glycans.

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Trypsin, MS Grade, G-Biosciences

Supplier: G-Biosciences

Trypsin is a serine endopeptidase that specifically cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues, and typically, there is little to no cleavage at arginyl-proline and lysyl-proline bonds

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EnGen Cas9 NLS, S. pyogenes, New England Biolabs

EnGen Cas9 NLS, S. pyogenes, New England Biolabs

Supplier: New England Biolabs (NEB)

EnGen Spy Cas9 NLS, is an RNA-guided endonuclease that catalyzes site- specific cleavage of double stranded DNA. The location of the break is within the target sequence 3 bases from the NGG PAM (Protospacer Adjacent Motif) (1). The PAM sequence, NGG, must follow the targeted region on the opposite strand of the DNA with respect to the region complementary sgRNA sequence. EnGen Spy Cas9 NLS contains Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein.

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Pierce™ Streptavidin, Maleimide-Activated, Thermo Scientific

Supplier: Invitrogen

Thermo Scientific Pierce Maleimide-Activated Streptavidin conjugate include streptavidin in a purified form, activated for crosslinking to sulfhydryl groups containing molecules.

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Pierce™ Protein Stabilizing Cocktail, Thermo Scientific

Pierce™ Protein Stabilizing Cocktail, Thermo Scientific

Supplier: Invitrogen

A versatile stabilizing solution that increases the shelf-life of purified or partially purified proteins during routine storage.

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Pierce™ Inclusion Body Solubilization Reagent, Thermo Scientific

Pierce™ Inclusion Body Solubilization Reagent, Thermo Scientific

Supplier: Invitrogen

Inclusion Body Solubilization Reagent effectively retrieves recombinant proteins that form insoluble aggregates during bacterial overexpression.

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Pierce® Pegylation Reagents, Methyl-PEG-Thiol Compound, Thermo Scientific

Pierce® Pegylation Reagents, Methyl-PEG-Thiol Compound, Thermo Scientific

Supplier: Invitrogen

Methyl-PEG4-thiol is a methyl- and sulfhydryl-terminated compound that contains a 4-unit polyethylene glycol (PEG) spacer and is used to modify surfaces such as quantum dots, monolayers and magnetic particles.

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Pierce™ Immobilized p-Aminophenyl Phosphoryl Choline Agarose, Thermo Scientific

Pierce™ Immobilized p-Aminophenyl Phosphoryl Choline Agarose, Thermo Scientific

Supplier: Invitrogen

Thermo Scientific Pierce p-Amino Phosphoryl Choline Agarose enables purification of C-reactive protein (CRP) from plasma, ascites and other biological fluids.

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Pierce™ Mass Spectrometry Reagents, MALDI Matrices, Single-Use, Thermo Scientific

Pierce™ Mass Spectrometry Reagents, MALDI Matrices, Single-Use, Thermo Scientific

Supplier: Invitrogen

MALDI Matrices are highly purified, recrystallized reagents packaged in convenient single-use tubes sufficient for analyzing up to 96 samples by matrix assisted laser desorption ionization.

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Pierce™ Pegylation Reagents, Methyl-PEG-NHS-Maleimide, Thermo Scientific

Pierce™ Pegylation Reagents, Methyl-PEG-NHS-Maleimide, Thermo Scientific

Supplier: Invitrogen

Pierce™ MM(PEG)n reagents are methyl-terminated PEG compounds activated with a maleimide group for covalent pegylation of sulfhydryls on proteins or assay surfaces.

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Pierce™ PEGylated N-succinimidyl S-acetylthioacetate, SAT(PEG)4, Thermo Scientific

Pierce™ PEGylated N-succinimidyl S-acetylthioacetate, SAT(PEG)4, Thermo Scientific

Supplier: Invitrogen

Pierce™ SAT(PEG)₄ (PEGylated N-succinimidyl S-acetylthioacetate) is a sulphhydryl-addition reagent for covalent modification of primary amines.

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Pierce™ NeutrAvidin™ Protein, Thermo Scientific

Supplier: Invitrogen

Pierce™ NeutrAvidin™ Protein is a specially-prepared form of avidin biotin-binding protein that decreases background in biotin-binding.

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HCCA Matrix, Bruker

Supplier: BRUKER SCIENTIFIC, LLC MS

Pre-portioned HCCA matrix provides easy and convenient preparation of HCCA matrix solutions.

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Pierce™ Immobilised TCEP Disulfide Reducing Gel, Thermo Scientific

Pierce™ Immobilised TCEP Disulfide Reducing Gel, Thermo Scientific

Supplier: Invitrogen

Immobilised TCEP Disulfide Reducing Gel eliminates the need to use laborious and inefficient gel filtration methods to separate the reduced sample from the reducing agent.

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