The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.
T3 DNA Ligase, New England Biolabs
Supplier: New England Biolabs (NEB)
T3 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T3.
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Seamless Assembly Master Mix (2X)
Supplier: ReVacc Scientific
This Seamless Assembly Master Mix (2X) uses Gibson cloning principle to allow efficiently assembly of multiple DNA fragments, regardless of fragment length or end compatibility. The insert and the linearized vector need the overlapped sequences of 15 bp - 20 bp on both 5' - and 3' -end, respectively. A positive control with the mixture of insert and linearized vector is included.
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Taq DNA Ligase, New England Biolabs
Supplier: New England Biolabs (NEB)
Taq DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5´ phosphate and 3´ hydroxyl termini of two adjacent oligonucleotides which are hybridized to a complementary target DNA
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ig-Fusion™ Cloning Kit, Intact Genomics
Supplier: INTACT GENOMICS, INC MS
Propriety ig-Fusion™ cloning technology is a simple, rapid and highly efficient cloning kit which allows to directly clone any PCR products to any linearized expression vector at any site.
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T4 DNA Ligase, Intact Genomics
Supplier: INTACT GENOMICS, INC MS
T4 DNA ligase displays significantly higher ligation efficiency than the nearest competitor.
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T4 DNA Ligase, Intact Genomics
Supplier: INTACT GENOMICS, INC MS
T4 DNA ligase displays significantly higher ligation efficiency than the nearest competitor.
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Ig-Fusion Cloning Enzyme Premix, Intact Genomics
Supplier: INTACT GENOMICS, INC MS
Intact Genomics propriety Ig-Fusion™ cloning technology is a simple, rapid and highly efficient cloning kit which allows to directly clone any PCR product(s) to any linearized expression vector at any site.
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Taq DNA Ligase (from Thermus aquaticus cloned Escherichia coli), Intact Genomics
Supplier: INTACT GENOMICS, INC MS
Taq ligase is thermostable DNA ligase from the thermophilic bacterium Thermus aquaticus cloned to E. coli cells.