You searched for: Cloning Kits and Ligation

Supplier: New England Biolabs (NEB)

This PCR Cloning Kit contains an optimized 2X Cloning Master Mix with a proprietary ligation enhancer and a linearized vector that uses a novel mechanism for background colony suppression to give a low background.

Supplier: New England Biolabs (NEB)

The Quick Blunting Kit is used to convert DNA with incompatible 5´ or 3´ overhangs to 5´ phosphorylated, blunt-ended DNA for efficient blunt-end ligation into DNA cloning vectors

Supplier: Agilent Technologies

The StrataClone PCR Cloning Kit allows high-efficiency, 5-minute cloning of PCR products at room temperature, using the efficient DNA rejoining activity of DNA topoisomerase I and the DNA recombination activity of Cre recombinase.

Supplier: Rockland Immunochemical

DNA Ligase T4 Molecular Biology Grade

Supplier: TELESIS BIO

Generate seamless plasmid DNA or BAC clones without restriction digestion.

Supplier: TELESIS BIO

The Select DNA cloning and amplification kits generate a minimum of 10 µg of amplified DNA from fragments of 0.3 to 1.8 kb using the BioXp® 3250.

Supplier: TELESIS BIO

BioXp Select plasmid amplification kits contain reagents for isothermal amplification of plasmid DNA.

Supplier: Agilent Technologies

The GeneMorph II random mutagenesis kits have been formulated to produce a more uniform mutational spectrum when performing error prone PCR to achieve random mutagenesis.

Supplier: TELESIS BIO

The Select DNA cloning Gibson assembly kits can generate Gibson clones starting with fragments and vector supplied by the end user.

Supplier: TELESIS BIO

The Select DNA cloning Golden Gate Assembly BsmBI kits are compatible with the Type IIS restriction enzymes BsmBI. Please choose the appropriate kit based on the number of fragments being assembled.

Supplier: New England Biolabs (NEB)

This PCR Cloning Kit contains an optimized Cloning Mix containing a proprietary ligation enhancer and a linearized vector that uses a novel mechanism for background colony suppression to give a low background.

Supplier: New England Biolabs (NEB)

The NEB Cloning Competent E.coli Sampler allows to sample four of popular chemically competent strains.

Supplier: TELESIS BIO

Generate seamless plasmid DNA or BAC clones without restriction digestion.

Supplier: TELESIS BIO

The Select DNA cloning and amplification kits generate a minimum of 10 µg of amplified DNA from fragments of 0.3 to 1.8 kb using the BioXp 9600.

Supplier: TELESIS BIO

BioXp Select plasmid amplification kits contain reagents for isothermal amplification of plasmid DNA.

Supplier: Agilent Technologies

Pfu DNA ligase is a recombinant enzyme derived from Pyrococcus furiosus hyperthermophilic marine archaebacterium that catalyzes linkage of adjacent 5'-phosphate and 3'-hydroxy ends of double-stranded DNA at 45 to 80 °C, with a temperature optimum near 70 °C for nick-sealing reactions.

Supplier: TELESIS BIO

The Select DNA cloning golden gate assembly, BsaI kits are compatible with the Type IIS restriction enzymes BsaI. Please choose the appropriate kit based on the number of fragments being assembled.

Supplier: Agilent Technologies

For libraries constructed from highly methylated DNA, Gigapack III Packaging Extract simplifies the packaging procedure and increases the efficiency and representation of libraries constructed from highly methylated DNA. Each packaging extract is restriction minus to optimize packaging efficiency and library representation.

Supplier: Agilent Technologies

The original QuikChange Site-Directed Mutagenesis Kits speed up and simplify site-directed mutagenesis studies. The kits eliminate the need for sub-cloning into M13-based bacteriophage vectors and for ss-DNA rescue. This allows oligo-mediated introduction of site-specific mutations into virtually any double-stranded plasmid DNA. In addition, the XL version of the kit is specially designed for efficient mutagenesis of large (4 -14 kb) or otherwise difficult-to mutagenize plasmid templates. The XL kit features components specifically designed for more efficient DNA replication and bacterial transformation.

Supplier: New England Biolabs (NEB)

This NEB Golden Gate Assembly Kit (BsaI-HFv2) contains an optimized mix of BsaI-HFv2 and T4 DNA Ligase. Together these enzymes can direct the assembly of multiple inserts/modules using the Golden Gate approach. The pGGA destination plasmid (included), provides a backbone for your assembly, features convenient restriction enzyme sites for subcloning, and has T7/SP6 promoter sequences to enable in vitro transcription.

Supplier: Lucigen

Highly specific thermostable DNA ligase for high-temperature applications

Supplier: Lucigen

The NxGen® T4 DNA Ligases from LGC Biosearch (Lucigen) come in a range of highly pure grades specifically for next-generation sequencing (NGS). This enzyme catalyses the formation of a phosphodiester bond between the terminal 5′ phosphate and 3′ hydroxyl groups of duplex DNA or RNA and efficiently joins blunt and cohesive ends, and repairs single stranded nicks in duplex DNA, RNA, or DNA/RNA hybrids.

Supplier: New England Biolabs (NEB)

The NEBNext Ultra II Ligation Module has been optimized to efficiently ligate DNA adaptors compatible with Illumina® sequencing to end-repaired, dA-tailed DNA fragments

Supplier: New England Biolabs (NEB)

T4 RNA Ligase 2, truncated (T4 Rnl2 truncated) specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ end of RNA.

Supplier: New England Biolabs (NEB)

RtcB Ligase is expressed as a His-tagged fusion in E. coli.

Supplier: Promega Corporation

For ligation of DNA ends, mutagenesis and cDNA cloning.

Supplier: New England Biolabs (NEB)

The Quick Ligation Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature

Supplier: Promega Corporation

T4 DNA Ligase and buffer combination for rapid (5-minute) ligations.

Supplier: Promega Corporation

For ligation of DNA ends.

Supplier: New England Biolabs (NEB)

Instant Sticky-end Ligase Master Mix is a ready-to-use 2X solution of T4 DNA ligase and a proprietary ligation enhancer in an optimized reaction buffer.