You searched for: Nucleic Acid Reagents

Supplier: Promega Corporation

T4 DNA Ligase and buffer combination for rapid (5-minute) ligations.

Supplier: New England Biolabs (NEB)

Thermostable 5´ App DNA/RNA Ligase is a point mutant of catalytic lysine of RNA ligase from Methanobacterium thermoautotrophicum

Supplier: New England Biolabs (NEB)

Gibson Assembly was developed by Dr

Supplier: New England Biolabs (NEB)

The Quick Ligation Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature

Supplier: New England Biolabs (NEB)

T4 RNA Ligase 2, also known as T4 Rnl2 (gp24.1), has both intermolecular and intramolecular RNA strand joining activity

Supplier: EMPIRICAL BIOSCIENCE

T4 DNA Ligase - High Concentration

Supplier: New England Biolabs (NEB)

Blunt/TA Ligase Master Mix is a ready-to-use solution of T4 DNA Ligase, proprietary ligation enhancer, and optimized reaction buffer.

Supplier: New England Biolabs (NEB)

T4 RNA Ligase 2, truncated K227Q (T4 Rnl2tr K227Q) specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ OH end of RNA.

Supplier: New England Biolabs (NEB)

A salt tolerant variant of T4 DNA Ligase, Salt-T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5 phosphate and 3 hydroxyl termini in duplex DNA or RNA and is designed to function at higher salt concentrations than wild type T4 DNA Ligase.

Supplier: New England Biolabs (NEB)

Instant Sticky-end Ligase Master Mix is a ready-to-use 2X solution of T4 DNA ligase and a proprietary ligation enhancer in an optimized reaction buffer.

Supplier: New England Biolabs (NEB)

Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA.

Supplier: Promega Corporation

The Flexi Vector System provides a rapid, efficient, high-fidelity way to transfer protein-coding regions without the need to resequence.

Supplier: New England Biolabs (NEB)

The NEBuilder HiFi DNA Assembly Cloning Kit combines the power of the NEBuilder HiFi DNA Assembly Master Mix with NEB 5-alpha Competent E

Supplier: New England Biolabs (NEB)

Catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA containing cohesive ends.

Supplier: Promega Corporation

Used for high-level expression of genes cloned into vectors for expression of sequence downstream from the T7 promoter, if the sequence contains a ribosomal binding site. No blue/white selection.

Supplier: New England Biolabs (NEB)

T4 RNA Ligase 2, truncated KQ (T4 Rnl2tr R55K, K227Q) specifically ligates the preadenylated 5´ end of DNA or RNA to the 3´ OH end of RNA.

Supplier: New England Biolabs (NEB)

NEBuilder HiFi DNA Assembly was developed to improve the efficiency and accuracy of DNA assembly

Supplier: New England Biolabs (NEB)

The NEBNext Ultra II Ligation Module has been optimized to efficiently ligate DNA adaptors compatible with Illumina® sequencing to end-repaired, dA-tailed DNA fragments

Supplier: New England Biolabs (NEB)

T4 RNA Ligase 2, truncated (T4 Rnl2 truncated) specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ end of RNA.

Supplier: Promega Corporation

Useful host for transformation of pGEM Vectors and production of ssDNA from M18 or phagemid vectors. JM109 is recA- and lacks the E. coli K restriction system. Can be used for blue/white screening.

Supplier: Promega Corporation

HaloTag Cloning Starter Kit: designed to use Flexi Cloning System to clone protein coding sequences into mammalian HaloTag expression vectors; clone and transfer gene sequences into C- or N-terminus of HaloTag Flexi CMV Deletion Vectors.

Supplier: New England Biolabs (NEB)

RtcB Ligase is expressed as a His-tagged fusion in E. coli.

Supplier: New England Biolabs (NEB)

Taq DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5´ phosphate and 3´ hydroxyl termini of two adjacent oligonucleotides which are hybridized to a complementary target DNA

Supplier: ReVacc Scientific

This Seamless Assembly Master Mix (2X) uses Gibson cloning principle to allow efficiently assembly of multiple DNA fragments, regardless of fragment length or end compatibility. The insert and the linearized vector need the overlapped sequences of 15 bp - 20 bp on both 5' - and 3' -end, respectively. A positive control with the mixture of insert and linearized vector is included.

Supplier: New England Biolabs (NEB)

NEBridge Ligase Master Mix is a 3X master mix for Golden Gate Assembly. Designed for use with NEB Type IIS restriction enzymes, this master mix contains T4 DNA Ligase in an optimized reaction buffer with a proprietary ligation enhancer.

Supplier: New England Biolabs (NEB)

T3 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T3.

Supplier: New England Biolabs (NEB)

SplintR Ligase, also known as PBCV-1 DNA Ligase or Chorella virus DNA Ligase, efficiently catalyzes the ligation of adjacent, single-stranded DNA oligonucleotides splinted by a complementary RNA strand.

Supplier: New England Biolabs (NEB)

The NEBNext® Ultra™ Ligation Module is designed for use with the NEBNext Singleplex or Multiplex Oligos for Illumina.

Supplier: Promega Corporation

T4 RNA Ligase catalyzes the ATP-dependent ligation of single-stranded RNA or DNA onto the 5'-phosphoryl termini of single-stranded RNA or DNA.

Supplier: New England Biolabs (NEB)

T7 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T7.