The electrophoresis buffers maintain consistent pH levels during DNA, RNA, and other protein separation. Providing ions that transfer the applied current, the low charge solutions facilitate different molecular sizes to travel at various rates for analyses. Offering additional protection to proteins, the preparative substances prevent degradation for guaranteed sample viability and accurate testing. Available in various molecular weights, the best electrophoresis buffer can be selected based on the specific macromolecules being handled.
ELISA Blocker Blocking Buffer, Thermo Scientific, Thermo Scientific
Supplier: Invitrogen
ELISA Blocker Blocking Buffer is a protein buffer that effectively blocks plate binding sites that remain in polystyrene microplate wells following initial coating steps in ELISA procedures. The ready-to-use buffer is optimized and routinely used for sandwich ELISA procedures involving cytokines, such as many of the Thermo Scientific Pierce ELISA Kits.
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StabilBlot™ Casein Blocker-5X, TBS, BioFX®, Surmodics IVD, Inc.
Supplier: Surmodics
The StabilBlot™ Blocker Family offers a range of options, both protein-based and synthetic-based, to block non-specific binding as well as enhance sensitivity when used as a blocker and antibody diluent.
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StabilCoat® BSA ELISA Stabilizer/Blocker, Surmodics
Supplier: Surmodics
StabilCoat® Immunoassay Stabilizer, and the 5X concentrate, effectively preserves the conformation and activity of dried proteins while simultaneously blocking the surface to reduce non-specific binding. The product can be used to stabilize antibodies, antigens or enzymes on an assortment of immunoassay components including polystyrene plates, tubes, glass, membranes, and filter paper.
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Tris Buffered Saline/Casein Block & Diluent -10X, BioFX®, Surmodics IVD, Inc.
Supplier: Surmodics
BioFX® Tris Buffered Saline/Casein Block and Diluent-10X Concentrate minimizes non-specific interactions of the secondary reactants with each other and with the primary solid phase binding sites. The high blocking efficiency of casein is due to its content of small molecular weight proteins.
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Assay Diluent (Protein-Free), Surmodics IVD, Inc.
Supplier: Surmodics
The Surmodics® Assay Diluent (Protein-Free) is a universal blocking agent that utilizes synthetic components to reduce matrix interference within immunoassay applications. Increased signal-to-noise ratios are produced when using the product with samples containing heterophilic antibodies (HAMA and rheumatoid factor).
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Phosphate Buffered Saline/Casein Block & Diluent-5X, BioFX®, Surmodics IVD, Inc.
Supplier: Surmodics
BioFX® Phosphate Buffered Saline/Casein Block and Diluent-5X Concentrate minimizes non-specific interactions of the secondary reagents with each other and the primary solid phase binding sites. The high blocking efficiency of casein is due to its content of small molecular weight proteins. The solution is ready for dilution to specific application requirements.
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ELISA Wash Buffer (30X), Thermo Scientific
Supplier: Invitrogen
ELISA Wash Buffer is a Tris-based wash buffer for use in cytokine and other sandwich ELISA procedures. The buffer is the same formulation that is supplied with most Thermo Scientific Pierce ELISA Kits for cytokines and chemokines. The 30X concentrated stock solution is easily diluted 1:30 (vol:vol) in ultrapure water to yield sufficient wash buffer for a typical 96-well microplate ELISA protocol. Supplement an existing ELISA Kit or use as a convenient general purpose wash buffer for customized ELISA methods.
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Pierce™ Fish Serum Blocking Buffe, Thermo Scientific
Supplier: Invitrogen
This Fish Serum Blocking Buffer is Steelhead Salmon Serum in PBS that is especially useful as a blocking agent in IHC and other detection methods involving mammalian samples.
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EndoTrap® HD 1/1, Biovendor
Supplier: BioVendor
EndoTrap® HD removes endotoxins (lipopolysaccharides/LPS) from aqueous solutions of a wide range of samples such as proteins, antibodies, vaccines, nucleic acids, buffers and cell extracts with excellent sample recovery rate.
The EndoTrap® HD resin is especially designed to meet the needs of pharma and biotech industry for the efficient removal of endotoxins in biomanufacturing processes as well as any application that requires highest endotoxin removal rates.
EndoTrap® HD fulfils all requirements for industrial applications, and it can easily be integrated in/at early or late-stage purification processes. Moreover, the High-Definition Matrix is also available for small-scale purification as pre-filled columns.
Endotoxin removal with EndoTrap® HD works effectively in the pH range of 4-9 and NaCl concentrations of 50-600 mM. Buffers such as HEPES, TRIS, MOPS, MES, and PIPES are recommended. Citrate buffers and chelators of divalent cations (like EDTA) have to be avoided
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Endo Trap® HD Buffer Kit, BioVendor
Supplier: BioVendor
EndoTrap® HD removes endotoxins (lipopolysaccharides/LPS) from aqueous solutions of a wide range of samples such as proteins, antibodies, vaccines, nucleic acids, buffers and cell extracts with excellent sample recovery rate.
The EndoTrap® HD resin is especially designed to meet the needs of pharma and biotech industry for the efficient removal of endotoxins in biomanufacturing processes as well as any application that requires highest endotoxin removal rates.
EndoTrap® HD fulfils all requirements for industrial applications, and it can easily be integrated in/at early or late-stage purification processes. Moreover, the High-Definition Matrix is also available for small-scale purification as pre-filled columns.
Endotoxin removal with EndoTrap® HD works effectively in the pH range of 4-9 and NaCl concentrations of 50-600 mM. Buffers such as HEPES, TRIS, MOPS, MES, and PIPES are recommended. Citrate buffers and chelators of divalent cations (like EDTA) have to be avoided
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Aquatic Block™ PBS
Supplier: Arlington Scientific
A non-mammalian blocking reagent supplied in PBS for diagnostic systems involving membrane or other solid phase material.
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Aqua Block™ Tris
Supplier: Arlington Scientific
A non-mammalian blocking reagent supplied in tris for diagnostic systems involving membrane or other solid phase material.
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Balance Blocker™ PBS
Supplier: Arlington Scientific
A proprietary blocking reagent supplied in tris for diagnostic systems involving membrane or other solid phase material.
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Balance Blocker™ Tris
Supplier: Arlington Scientific
A universal blocking reagent supplied in tris for diagnostic and research systems.
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Balance Blocker™ PBS (with Surfactant)
Supplier: Arlington Scientific
A universal blocking reagent supplied in PBS for diagnostic and research systems.
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Blocking Buffer Sample Pack
Supplier: Arlington Scientific
Contains: Aquatic Block PBS, Aquatic Block Tris, Aquatic Block Serum Free PBS, Aquatic Block Serum Free Tris,
Balance Blocker PBS, Balance Blocker Tris.
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Aquatic Block™ Serum-Free Tris (with Surfactant)
Supplier: Arlington Scientific
A proprietary blocking reagent supplied in tris for diagnostic systems involving membrane or other solid phase material.
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Aqua Block™ PBS
Supplier: Arlington Scientific
A non-mammalian blocking reagent supplied in PBS for diagnostic systems involving membrane or other solid phase material.
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Aquatic Block™ PBS, Azide-Free
Supplier: Arlington Scientific
A non-mammalian blocking reagent supplied in PBS for diagnostic systems involving membrane or other solid phase material.
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Aquatic Block™ Serum-Free PBS (with Surfactant)
Supplier: Arlington Scientific
A non-mammalian blocking reagent supplied in PBS for diagnostic systems involving membrane or other solid phase material.
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Aquatic Block™ Serum-Free PBS, Azide-Free (with Surfactant)
Supplier: Arlington Scientific
A non-mammalian blocking reagent supplied in PBS for diagnostic systems involving membrane or other solid phase material.
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Aquatic Block™ Tris
Supplier: Arlington Scientific
A non-mammalian blocking reagent supplied in tris for diagnostic systems involving membrane or other solid phase material. This new reagent imparts superior stability and flow characteristics, while utilizing proteins that do not interact with mammalian antibodies.