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Cell Culture Buffers

Tissue culture buffers provide an energy source, maintain proper pH levels, and keep ideal osmotic pressure present. Used predominately in biomolecular research, the dry and liquid substances have a balanced inorganic salt composition. Lower concentration options permit equilibration with air in a closed system, while higher salt concentration options keep gaseous phases in equilibrium. Cell growth is optimal when plated in a nutritional medium that has been buffered.

VWR® Sodium Phosphate 0.1 M, pH 7.0 Buffer

VWR® Sodium Phosphate 0.1 M, pH 7.0 Buffer

Supplier: VWR International

Made with sodium phosphate monobasic monohydrate, sodium hydroxide solution and deionized water.

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VWR® Blood Bank Saline Solutions

VWR® Blood Bank Saline Solutions

Supplier: VWR International

Blood bank saline is pH-controlled for a pH between 6.5 and 7.0.

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VWR® TRIS Buffer, 0.1 M Solution, pH 7.4

VWR® TRIS Buffer, 0.1 M Solution, pH 7.4

Supplier: VWR

Cell culture buffer, TRIS Buffer Solution 0.1M, pH 7.4

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VWR® Phosphate Buffered Saline (PBS) 20X, Ultra Pure Grade

VWR® Phosphate Buffered Saline (PBS) 20X, Ultra Pure Grade

Supplier: VWR

1X Solution Composition:135 mM NaCl, 2.7 mM KCl,11 mM Phosphate Buffer.Does not contain magnesium or calcium.

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VWR® Phosphate Buffered Saline, Ultra Pure Grade

VWR® Phosphate Buffered Saline, Ultra Pure Grade

Supplier: VWR

1X Solution Composition: 137 mM NaCl, 2.7 mM KCl, 9.8 mM Phosphate buffer

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VWR® Phosphate Buffer Solution

VWR® Phosphate Buffer Solution

Supplier: VWR

Phosphate Buffered Saline (PBS) with 0.05% Tween® 20, pH 7.5 is a ready-to-use 1X concentration

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VWR® TRIS Buffer, 1 M Solution, pH 8.0

VWR® TRIS Buffer, 1 M Solution, pH 8.0

Supplier: VWR

Tris (hydroxymethyl) aminomethane

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Phosphate Buffered Saline (PBS),Powdered,  Ultra Pure Grade

Phosphate Buffered Saline (PBS),Powdered, Ultra Pure Grade

Supplier: VWR

1X Solution Composition:137 mM NaCl,2.7 mM KCl,9.8 mM Phosphate buffer
May be sterilized by filtration.
Does not contain magnesium or calcium.
pH at 25°C (1X PBS): 7.3 – 7.5
Conductivity at 25°C (1X PBS): 13,500-17,000 umhos

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VWR® Phosphate Buffered Saline (PBS), 10X, Ultra Pure Grade

VWR® Phosphate Buffered Saline (PBS), 10X, Ultra Pure Grade

Supplier: VWR

1X Solution Composition: 137 mM NaCl, 2.7 mM KCl, 9.5 mM Phosphate buffer.

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VWR® Ultra Pure Grade Phosphate Buffered Saline

VWR® Ultra Pure Grade Phosphate Buffered Saline

Supplier: VWR

1X Solution Composition:137 mM NaCl, 2.7 mM KCl, 9.5 mM Phosphate buffer

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Phosphate Buffered Saline

Phosphate Buffered Saline

Supplier: Ward's Science

Phosphate Buffered Saline

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Dulbecco's phosphate-buffered saline (DPBS), Mediatech

Dulbecco's phosphate-buffered saline (DPBS), Mediatech

Supplier: Corning

Dulbecco's Phosphate Buffered Saline (DPBS) is a buffer solution which can be used to maintain cell culture media in the physiological pH range of 7.0 to 7.6

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Corning® HEPES buffer solution (1 mol/L)

Corning® HEPES buffer solution (1 mol/L)

Supplier: Corning

HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulphonic acid) provides an alternative to the use of bicarbonate for buffering because it does not require elevated levels of CO2

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PBS 10X-Dulbecco's Phosphate Buffered Saline Solution

PBS 10X-Dulbecco's Phosphate Buffered Saline Solution

Supplier: FUJIFILM IRVINE SCIENTIFIC INC.

PBS 10X Dulbecco's Phosphate buffered saline solution can be used to provide a buffer system to maintain cell culture media in the physiological pH range, or to irrigate, transport, or dilute fluid while maintaining cell tonicity and viability.

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Corning® Sodium Bicarbonate, Corning

Corning® Sodium Bicarbonate, Corning

Supplier: Corning

Sodium bicarbonate is used as part of a buffering system commonly used to maintain physiological pH 7.2 - 7.4 in a culture environment

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Corning® Phosphate-Buffered Saline (PBS), 10X Concentrate, pH 7,4, Molecular Biology Grade

Corning® Phosphate-Buffered Saline (PBS), 10X Concentrate, pH 7,4, Molecular Biology Grade

Supplier: Corning

Phosphate Buffered Saline (PBS) is used to maintain a physiological pH and osmolarity, two key factors in tissue culture and molecular biology research

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Phosphate Buffered Saline (PBS), Quality Biological

Phosphate Buffered Saline (PBS), Quality Biological

Supplier: QUALITY BIOLOGICAL, INC.

PBS (phosphate buffered saline) is a balanced salt solution used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.

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Phosphate Buffered Saline (10X PBS), pH 7.2, Sterile, Quality Biological

Phosphate Buffered Saline (10X PBS), pH 7.2, Sterile, Quality Biological

Supplier: QUALITY BIOLOGICAL, INC.

PBS (phosphate buffered saline) is a balanced salt solution used for a variety biological and cell culture applications.

    
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HEPES Buffer Solution 1 M

HEPES Buffer Solution 1 M

Supplier: FUJIFILM IRVINE SCIENTIFIC INC.

Buffer to maintain pH between 7.2 and 7.6. Suitable for use in cell cultures outside of a CO₂ incubator

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Corning® 3M™ Sodium Acetate, Corning

Corning® 3M™ Sodium Acetate, Corning

Supplier: Corning

Sodium Acetate is a common molecular biology reagent. All production lots are tested to ensure that they are free of RNAse. It is often used in buffer systems to maintain the pH of a given solution.

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1X Phosphate Buffered Saline without Magnesium and Calcium, MP Biomedicals

1X Phosphate Buffered Saline without Magnesium and Calcium, MP Biomedicals

Supplier: MP Biomedicals

Phosphate Buffered Salts, Dulbecco's Formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells.

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Corning® PBS (Phosphate-Buffered Saline), Corning

Corning® PBS (Phosphate-Buffered Saline), Corning

Supplier: Corning

Phosphate buffered saline (PBS) is a buffer solution containing sodium chloride, sodium phosphate, and in some formulations potassium chloride and potassium phosphate

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Corning® EDTA, 0.5M pH 8.0, Corning

Corning® EDTA, 0.5M pH 8.0, Corning

Supplier: Corning

EDTA (ethylenediaminetetraacetic acid) is a polyamino carboxylic acid. It is a colorless, water-soluble solid and is useful as a chelating agent due to its ability to "sequester" metal ions such as Ca2+ and Fe3+.  These cations are responsible for various undesirable effects in molecular biological and cell culture processes. After being bound by EDTA, these metal ions remain in the solution but exhibit diminished reactivity.  The use of EDTA therefore allows for greater control over many experiments.

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Corning® HBSS (Hanks Balanced Salt Solution), Corning

Corning® HBSS (Hanks Balanced Salt Solution), Corning

Supplier: Corning

Hanks' Balanced Salt Solution (HBSS) is a buffer used to maintain a physiological pH for cells maintained in non-CO₂ atmospheric conditions.

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PBS 1X-Dulbecco's Phosphate Buffered Saline Solution

PBS 1X-Dulbecco's Phosphate Buffered Saline Solution

Supplier: FUJIFILM IRVINE SCIENTIFIC INC.

PBS 1X Dulbecco's Phosphate Buffered Saline Solution can be used to provide a buffer system to maintain cell culture media in the physiological pH range, or to irrigate, transport, or dilute fluid while maintaining cell tonicity and viability.

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10X Phosphate Buffered Saline (PBS), Sterile,  Dulbecco's Formula, without Magnesium and Calcium, MP Biomedicals

10X Phosphate Buffered Saline (PBS), Sterile, Dulbecco's Formula, without Magnesium and Calcium, MP Biomedicals

Supplier: MP Biomedicals

Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions: 1. Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance; 2. Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism; 3. Combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism; 4. Provides a buffering system to maintain the medium within the physiological pH range (7.2 - 7.6)

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Corning® Tris, 10X Buffered Saline, Liquid, Corning

Corning® Tris, 10X Buffered Saline, Liquid, Corning

Supplier: Corning

Tris Buffered Saline (TBS) is an isotonic, non-toxic buffer used in some biomedical techniques to maintain pH within a relatively narrow range

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HEPES Buffer, 1 M Sterile, Cell Culture Grade, MP Biomedicals

HEPES Buffer, 1 M Sterile, Cell Culture Grade, MP Biomedicals

Supplier: MP Biomedicals

HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a general purpose zwitterionic organic chemical buffering agent which does not bind magnesium, calcium, manganese(II) or copper (II) ions.

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1X PBS (Phosphate Buffered Saline), pH 7.4

Supplier: GROWCELLS

Balanced salt solution used in many biomolecular applications

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HBSS 10X-Hanks' Balanced Salt Solution

HBSS 10X-Hanks' Balanced Salt Solution

Supplier: FUJIFILM IRVINE SCIENTIFIC INC.

HBSS 10X Hanks' Balanced salt solution is designed to maintain pH and osmotic balance and to provide cells with water and essential inorganic ions. HBSS is suitable for use in sealed culture flasks incubated in air.

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