Chloroform is typically used as a solvent and as a cleansing agent. Procedures have been described for the use of chloroform in lambda plaques storage, lambda cDNA storage, the removal of mineral oil from PCR reaction samples, oligonucleotide purification, a hydroxyl radical footprinting protocol, a transcriptional run-on assay protocol, and an overlay assay for beta-galactosidase activity. It has also been used with phenol in such procedures as DNA recovery from polyacrylamide gels, ethidium bromide removal from DNA preparations, lysis protocols for plasmid DNA isolation, RNase removal, and purification of yeast DNA. A protocol describes the use of chloroform in a high-performance thin-layer chromatography protocol for sphingomyelin analysis. Chloroform can also be used in chloroform/methanol mixtures for the isolation of cardiolipids from Geobacillus stearothermophilus and their subsequent MS analysis. The isolation of the bacteriocin amylovorin L471 from Lactobacillus amylovorus DCE 471 in culture broth has been reported, using chloroform/methanol extraction and precipitation in the procedure. Chloroform/2-butanol mixtures can be used for the extraction of steroid sulfates for analysis by nanoelectrospray ionization mass spectrometry.

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Chloroform 99+% stabilized, Extra Pure

Supplier: Thermo Scientific Chemicals

Chloroform 99+% stabilised, extra pure

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Chloroform 99% stabilized

Supplier: Thermo Scientific Chemicals

Trichloromethane. Grade: 99. Melting Point C-64 to -62*. Boiling Point C: 60-62*. CHCl3. 67-66-3. Stabilised with ca 1% ethanol. POSSIBLE CARCINOGEN HARMFUL

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Chloroform ≥99.8% stabilized ACS, meets analytical specifications of Reagent Ph. Eur.

Supplier: Thermo Scientific Chemicals

Chloroform ≥99.8% stabilised ACS, meets analytical specifications of Reagent Ph. Eur.

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Chloroform, Hydranal™ solubilizer for Karl Fischer titration, Fluka™

Supplier: Honeywell Research Chemicals

Hydranal* Chloroform, CAS Number: 67-66-3, Molar Weight: 18.02 g/mol, Impurities less than or equal to 0.01% water (Karl Fischer), Suitability: use tested, Solvent for KF titration, Synonym: Methylidyne trichloride; Trichlorometha

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Chloroform 99+% stabilized ethanol-free

Supplier: Thermo Scientific Chemicals

Trichloromethane. Grade: ethanol-free, 99+. Melting Point C-64 to -63*. Boiling Point C: 60-62*. CHCl3. 67-66-3. Stabilised with 60 ppm amylene. POSSIBLE CARCINOGEN HARMFUL

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Chloroform ≥99.8% stabilized, Environmental Grade

Supplier: Thermo Scientific Chemicals

Suitable for pesticide analysis, environmental and GC analysis

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Chloroform ≥99.5% stabilized

Supplier: TCI America

(stabilized with 2-Methyl-2-butene)
[for HPLC Solvent]
CAS Number: 67-66-3
MDL Number: MFCD00000826
Molecular Formula: CHCl3
Molecular Weight: 119.37
Purity/Analysis Method: >99.5% (GC)
Form: Clear Liquid
Boiling point (°C): 60
Melting point (°C): -64
Specific Gravity (20/20): 1.49

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Chloroform ≥99.0% stabilized

Supplier: TCI America

(stabilized with Ethanol)
[for Spectrophotometry]
CAS Number: 67-66-3
MDL Number: MFCD00000826
Molecular Formula: CHCl3
Molecular Weight: 119.37
Purity/Analysis Method: >99.0% (GC)
Form: Clear Liquid
Boiling point (°C): 60
Melting point (°C): -64
Specific Gravity (20/20): 1.49

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Chloroform 99.9% stabilized, ECD tested for residue analysis, for pesticide residue analysis residue <0.0005%

Supplier: Thermo Scientific Chemicals

Chloroform 99.9% stabilised, ECD tested for residue analysis, for pesticide residue analysis residue 0.0005%

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Chloroform ≥99.8%, OmniPur® for biotechnology

Supplier: MilliporeSigma

For molecular biology.

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Chloroform ≥99.0% stabilized

Supplier: TCI America

(stabilized with Ethanol)
[for HPLC Solvent]
CAS Number: 67-66-3
MDL Number: MFCD00000826
Molecular Formula: CHCl3
Molecular Weight: 119.37
Purity/Analysis Method: >99.0% (GC)
Form: Clear Liquid
Boiling point (°C): 60
Melting point (°C): -64
Specific Gravity (20/20): 1.48

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Chloroform ≥99.7%, Glass Distilled

Supplier: Electron Microscopy Sciences

Glass distilled chloroform is available for your convenience.

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Chloroform, extra dry 99.9% stabilized, AcroSeal™

Supplier: Thermo Scientific Chemicals

Chloroform, extra dry 99.9% stabilized, AcroSeal™

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Chloroform ACS

Supplier: Ricca Chemical

Chloroform, ACS Reagent Grade, CAS Number: 67-66-3, Molecular Formula: CHCl3, Molecular Weight: 119.37 g/mol, Appearance: Colorless liquid, Odor: Characteristic, pleasant, sweet, Physical State: Liquid, Size: 500 mL amber glass

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Chloroform ≥99% stabilized for molecular biology

Supplier: MP Biomedicals

Chloroform is a common laboratory reagent favored for use as a solvent due to the compounds relative unreactivity and miscibility with most organic compounds. Chloroform is a colorless, volatile chlorinated organic solvent whose vapors have a narcotic effect.
Chloroform is use as a solvent for fats, oils, rubber, alkaloids, waxes, gutta-percha, resins; as cleansing agent; in fire extinguishers to lower the freezing temperature of carbon tetrachloride. Procedures have been described for the use of chloroform in lambda plaques storage, lambda cDNA storage, the removal of mineral oil from PCR reaction samples, oligonucleotide purification, a hydroxyl radical footprinting protocol, a transcriptional run-on assay protocol, and an overlay assay for beta-galactosidase activity. It has also been used with phenol in such procedures as DNA recovery from polyacrylamide gels, ethidium bromide removal from DNA preparations, lysis protocols for plasmid DNA isolation, RNase removal, and purification of yeast DNA. A protocol describes the use of chloroform in a high-performance thin-layer chromatography protocol for sphingomyelin analysis. Chloroform can also be used in chloroform/methanol mixtures for the isolation of cardiolipids from Geobacillus stearothermophilus and their subsequent MS analysis. The isolation of the bacteriocin amylovorin L471 from Lactobacillus amylovorus DCE 471 in culture broth has been reported, using chloroform/methanol extraction and precipitation in the procedure. Chloroform/2-butanol mixtures can be used for the extraction of steroid sulfates for analysis by nanoelectrospray ionization mass spectrometry.
Physical Appearance: Liquid
Vapor Density: 4.36 (air = 1) (Lit.)
Vapor Pressure: 211 hPa at 20 °C(Lit.)
Refractive Index: n20/D 1.445(Lit.)
Solubility: Soluble in water (1 mL/200 mL water); miscible with ethanol, benzene, ether, petroleum ether, carbon tetrachloride, carbon disulfide, oils.

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Chloroform, extra dry ≥99.9% stabilized, AcroSeal™

Supplier: Thermo Scientific Chemicals

Chloroform, extra dry ≥99.9% stabilized, AcroSeal™

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Chloroform ≥99.8% stabilized ACS

Supplier: Thermo Scientific Chemicals

MDL: MFCD00000826 Beilstein Registry No.: 1731042 Fieser: 1,29 3,140 4,22 5,28 8,92 12,517 15,84

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Chloroform ≥99.5% for spectrophotometry

Supplier: Thermo Scientific Chemicals

MDL: MFCD00000826 Beilstein Registry No.: 1731042 Notes: Meets ACS Spectrophotometric Requirements. Filtered through 0.2µ filters. Fieser: 1,29 3,140 4,22 5,28 8,92 12,517 15,84

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Chloroform ≥99.8% stabilized ACS for HPLC

Supplier: BeanTown Chemical

CAS: 67-66-3; EC No: 200-663-8; MDL No: MFCD00000826; RTECS: FS9100000 UN No: UN1888; Haz Class: 6.1; Packing Group: III Liquid; Molecular Formula: CHCl3; MW: 119.38 Melting Point: -63°; Boiling Point: 60.5-61.5° Density (g/mL): 1.492; Refractive Index: 1.445