You searched for: Protein Extraction Kits

Supplier: Promega Corporation

TNT Coupled Reticulocyte Lysate Systems are single-tube, coupled transcription/translation systems for eukaryotic cell-free protein expression.

Supplier: G-Biosciences

G-Biosciences' HOOK™ Cell Surface Protein Isolation kit uses our HOOK™ biotin labeling and purification technology in conjunction with our Mammalian Cell PE LB™ lysis buffer to conveniently label cell surface proteins and isolate them for further analysis, including Western blotting

Supplier: Promega Corporation

Untreated Rabbit Reticulocyte Lysate contains the cellular components necessary for protein synthesis (tRNA, ribosomes, amino acids, initiation, elongation and termination factors) but has not been treated with micrococcal nuclease.

Supplier: Promega Corporation

FastBreak Cell Lysis Reagent allows efficient, gentle lysis of E. coli cultures without the need for centrifugation or mechanical disruption.

Supplier: Promega Corporation

The TNT Coupled Wheat Germ Extract Systems are one-tube, coupled transcription/translation systems for eukaryotic cell-free protein expression.

Supplier: Promega Corporation

TNT Coupled Reticulocyte Lysate Systems are single-tube, coupled transcription/translation systems for eukaryotic cell-free protein expression.

Supplier: Promega Corporation

Flexi Rabbit Reticulocyte Lysate System allows optimization of translation reactions for a range of parameters, including Mg(2+) and K(+) and DTT concentration.

Supplier: Promega Corporation

Rabbit Reticulocyte Lysate Translation Systems are used in identification of mRNA species, characterization of their protein products and investigation of transcriptional and translational control.

Supplier: Bio-Rad

The ReadyPrep protein extraction kit (cytoplasmic/nuclear) prepares fractions enriched in cytoplasmic and nuclear proteins from eukaryotic samples, such as cultured cells and tissues.

Supplier: Bio-Rad

The ReadyPrep protein extraction kit (membrane II) applies sodium carbonate extraction methods (Fujiki et al

Supplier: Invitrogen

The Mem-PER™ Eukaryotic Membrane Protein Extraction Kit enables small-scale solubilization and enrichment of hydrophobic membrane-associated proteins in a simple reagent-based procedure

Supplier: Invitrogen

Pierce™ TMT™ Isobaric Mass Tagging kits and reagents enable multiplex quantitation of proteins extracted from cells and tissues using tandem mass spectrometry

Supplier: Invitrogen

The NE-PER nuclear and cytoplasmic extraction kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours.

Supplier: Invitrogen

The lysosome enrichment kit for tissue and cultured cells enables isolation and enrichment for intact lysosomes from crude cell and tissue extracts. Upon receipt store at 4˚C. Product shipped at ambient temperature.

Supplier: Invitrogen

The Thermo Scientific Pierce Ubiquitin Enrichment Kit facilitates the isolation of polyubiquitin protein conjugates from cultured cells and tissue samples.

Supplier: Invitrogen

The Mitochondria Isolation Kit for Cultured Cells provides a versatile, microcentrifuge-tube method for fractionating intact mitochondria from cultured mammalian cell samples.

Supplier: Invitrogen

The Pierce™ Rapid Isotyping Kit is a 5-minute lateral-flow assay with ELISA sensitivity for class and subclass determination of monoclonal antibodies.

Supplier: Invitrogen

The Pierce™ Cell Surface Protein Isolation Kit is a complete set of reagents for selective biotinylation and subsequent purification of mammalian cell surface proteins to the exclusion of intracellular proteins.

Supplier: Invitrogen

The Pierce™ Mouse Embryonic Fibroblast Isolation Kit provides isolation and culturing reagents for the optimal yield and viability of fibroblasts from mouse embryos.

Supplier: Invitrogen

The Pierce™ Albumin/IgG Removal Kit is optimised to decrease the abundant albumin and antibody components of human serum samples in preparation for 2D electrophoresis and other protein profiling methods. By eliminating most of these two proteins, which account for nearly 70% of total serum proteins, other less abundant proteins of interest can be more easily detected and studied.

Supplier: Invitrogen

Thermo Scientific™ Pierce™ High pH Reversed-Phase Peptide Fractionation Kit increases protein identification from LC/MS analysis through orthogonal peptide fractionation of complex peptide samples.Easy-to-use—resin provided in single-use spin column formatImproved protein identifications—protein identifications increased by ≥50% when compared to unfractionated samplesReproducible—elution profiles and fractional resolution vary by less than 20%Optimized—robust procedure for maximal protein identification and peptide recovery while minimizing fractional overlapCompatible—reagents validated with a variety of complex samples, including TMT™-labeled peptidesIn order to enable deep proteome sequencing, it is often necessary to reduce the sample complexity by fractionation in an orthogonal dimension prior to LC/MS analysis. The Pierce High pH Reversed-Phase Peptide Fractionation Kit utilizes high pH reversed-phase chromatography to separate peptides by hydrophobicity and provides excellent orthogonality to low pH reversed-phase LC-MS gradients.The kit has been designed to improve protein identification through the use of a proprietary reversed-phase resin in an easy-to-use spin column format with a highpH fractionation protocol. In contrast to strong cation exchange (SCX) fractionation, the high-pH reversed-phase fractions do not require an additional desalting step before LC/MS analysis.The High pH Reversed-phase Peptide Fractionation Kit includes a high pH buffer (0.1% triethylamine) and twelve spin columns containing pH-resistant reversed-phase resin. Each reversed-phase fractionation spin column enables fractionation of 10–100 µg of peptide sample using a microcentrifuge.Native, phosphorylated, Tandem Mass TagTM (TMTTM)-labeled, and other complex peptide mixture samples can be fractionated using this kit. Combining the search results generated by the individual fractions helps improve protein sequence coverage and increases the number of identified proteins relative to unfractionated samples.Applications:Reducing sample complexity to identify targets of interestConducting systems biology studiesReducing sample complexity to improve quantitation studies

Supplier: Invitrogen

The Glycoprotein Isolation Kit allows isolation of glycoproteins from complex mixtures, including serum, tissue and cultured cell lysates.

Supplier: Invitrogen

The Pierce™ In-Gel Tryptic Digestion Kit provides a complete set of reagents for digesting and recovering peptide fragments from stained protein bands excised from SDS-PAGE (polyacrylamide gel plugs).

Supplier: Invitrogen

The Imject™ EDC Carrier Protein Spin Kits enable effective one-step conjugation of a hapten to a carrier protein using EDC as the crosslinker. The resulting conjugate is used for eliciting an immune response and antibody production against the hapten.

Supplier: Invitrogen

Pierce™ In-Solution Tryptic Digestion and Guanidination Kit contains proteomics grade trypsin, buffers and reagents to alkylate and digest proteins and then guanidinate the peptide fragments for mass spectrometry.

Supplier: Invitrogen

The Mitochondria Isolation Kit for Tissue enables isolation of intact mitochondria from soft and hard tissue samples.

Supplier: Invitrogen

The Thermo Scientific Imject Maleimide-Activated BSA Spin Kit contains stabilized, lyophilized Maleimide-Activated BSA (in PBS with stabilizer) and accessory components to easily prepare ready-to-use immunogens for immunization.

Supplier: Invitrogen

Phosphoprotein Enrichment Kit is designed for the efficient enrichment of phosphorylated proteins derived from mammalian cells and tissues.

Supplier: Invitrogen

The Pierce™ Primary Cardiomyocyte Isolation Kit provides isolation and culturing reagents for the optimal yield and viability of primary cardiomyocytes from neonatal heart from mouse and rat.

Supplier: Invitrogen

The Pierce™ Primary Neuron Isolation Kit provides isolation and culturing reagents for the optimal yield and viability of primary neurons from embryonic cortical and hippocampal tissue from mouse and rat.