You searched for: Enzyme Assays
Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
ADP-Glo Kinase Assay + PKC beta I Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human PKCbeta I was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PKCβI is a member of the PKC family (phospholipid-dependent serine/threonine kinase) and is highly related to PKCβ II.
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Amplite® Fluorimetric Catalase Assay Kit *Red Fluorescence* , AAT Bioquest
Supplier: AAT Bioquest
Catalase is a common antioxidant heme-containing redox enzyme found in nearly all living organisms that are exposed to oxygen.
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ADP-Glo Kinase Assay + PDGFR alpha (T674I) Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant human PDGFRα (Y674I) (550-end) point mutant was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
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ADP-Glo Kinase Assay + EGFR (T790M, L858R) Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant human EGFR (T790M, L858R) (amino acids 695-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. EGFR is receptor for members of the EGF family and a transmembrane glycoprotein that has tyrosine kinase activity.
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FLUOR DE LYS® HDAC3/NCOR1 Fluorometric Drug Discovery Kit, Enzo Life Sciences
Supplier: Enzo Life Sciences
The HDAC3/NCOR1 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC3 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions.
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Amplite® Colorimetric Beta-Galactosidase Assay Kit , AAT Bioquest
Supplier: AAT Bioquest
E. coli beta-galactosidase is a 464 kD tetramer.
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Amplite® Fluorimetric Glutathione Peroxidase Assay Kit *Blue Fluorescence* , AAT Bioquest
Supplier: AAT Bioquest
Glutathione peroxidase is an enzyme family with peroxidase activity to protect the organism from oxidative damage.
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FLUOR DE LYS®-Green HDAC Fluorometric Activity Assay Kit, Enzo Life Sciences
Supplier: Enzo Life Sciences
An improved Fluor de Lys® HDAC assay with Fluor de Lys®-Green, a new substrate offering higher sensitivity and an excitation and emission (485/530nm) that avoids quenching and fluorescent interference from compounds absorbing in the near UV and blue range.The Fluor de Lys®-Green HDAC assay is a complete kit for measuring histone deacetylase (HDAC) activity in cell or nuclear extracts, immunoprecipitates or purified enzymes
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Amplite® Fluorimetric Alkaline Phosphatase Assay Kit *Blue Fluorescence* , AAT Bioquest
Supplier: AAT Bioquest
Alkaline phosphatase is a highly sensitive enzyme for ELISA, immuno-histochemical, Northern, Southern and Western blot applications.
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Amplite® Luminometric Alkaline Phosphatase Assay Kit *Luminescence* , AAT Bioquest
Supplier: AAT Bioquest
Alkaline phosphatase is a highly sensitive enzyme for ELISA, immuno-histochemical, Northern, Southern and Western blot applications.
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Matrix Metalloproteinase (MMP) Inhibitor Profiling Kit, Fluorometric RED, Enzo Life Sciences
Supplier: Enzo Life Sciences
The MMP inhibitor profiling kit, fluorometric (also known as fluorimetric) RED is a complete assay system designed to examine the specificity of inhibitors against a panel of ten matrix metalloproteinase enzymes, using a quenched fluorogenic substrate OMNIMMP® RED: TQ3-GABA-Pro-Cha-Abu-Smc-His-Ala-Dab(6’-TAMRA)-Ala-Lys-NH2 [TQ3=quencher; GABA=4-aminobutyric acid; Cha=L-cyclohexylalanine; Abu=2-aminobutyric acid; Smc=S-methyl-L-cysteine; Dab=2,4-diaminobutyric acid; 6’-TAMRA=6’-tetramethylrhodamine]. TAMRA fluorescence is thoroughly quenched by the TQ3 group until cleavage by MMPs separates the two moieties.
The assays are performed in a convenient 96-well microplate format. The compound NNGH is also included as a prototypic control inhibitor.
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HDAC-Glo I/II Assay, Promega
Supplier: Promega Corporation
The HDAC-Glo I/II Assays and Screening System are single-reagent-addition, homogeneous, luminescent assays that measure the relative activity of histone deacetylase (HDAC) class I and II enzymes from cells, extracts or purified enzyme sources.
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Amplite® Colorimetric Sphingomyelinase Assay Kit *Blue Color* , AAT Bioquest
Supplier: AAT Bioquest
Sphingomyelinase (SMase) is an enzyme that is responsible for cleaving sphingomyelin (SM) to phosphocholine and ceramide.
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Amplite® Fluorimetric Acetylcholinesterase Assay Kit *Green Fluorescence* , AAT Bioquest
Supplier: AAT Bioquest
Acetylcholinesterase, also known as AChE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group.
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Amplite® Fluorimetric HDAC Activity Assay Kit *Green Fluorescence* , AAT Bioquest
Supplier: AAT Bioquest
Histone deacetylases (HDAC) are a class of enzymes that remove acetyl groups from a ε-N-acetyl lysine amino acid on a histone.
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Amplite® Fluorimetric Acidic Sphingomyelinase Assay Kit *Red Fluorescence* , AAT Bioquest
Supplier: AAT Bioquest
Sphingomyelinase (SMase) is an enzyme that is responsible for cleaving sphingomyelin (SM) to phosphocholine and ceramide.
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Matrix Metalloproteinase-14 (MMP-14) Colorimetric Drug Discovery Kit, Enzo Life Sciences
Supplier: Enzo Life Sciences
A QuantiZymeTM Assay System
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ADP-Glo Kinase Assay + ASK1 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant human ASK1 (649-946) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. ASK1, also known as MAPKKK5, activates MKK3, MKK4 (SEK1) and MKK6. Overexpression of ASK1 induces apoptotic cell death.
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Glutathione peroxidase activity kit, Enzo Life Sciences
Supplier: Enzo Life Sciences
For the measurement of glutathione peroxidase in cell lysates, plasma, and tissue from any species.
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DPPIV Drug Discovery Kit, Enzo Life Sciences
Supplier: Enzo Life Sciences
The DPPIV Drug Discovery Kit is a complete assay system designed to screen DPPIV inhibitors, a potential therapeutic target, providing enough material to perform at least 96 assays.
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Cathepsin S Drug Discovery Kit, Enzo Life Sciences
Supplier: Enzo Life Sciences
A QuantiZyme™ Assay System
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CV-Cathepsin L Detection Kit, Enzo Life Sciences
Supplier: Enzo Life Sciences
Based on the dye cresyl violet and the cathepsin L PheArg peptide recognition sequence, this kit allows the detection of cathepsin L activity in living cells by fluorescence microscopy.
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Matrix Metalloproteinase-1 (MMP-1) Fluorometric Drug Discovery Kit, Enzo Life Sciences
Supplier: Enzo Life Sciences
A QuantiZymeTM Assay System
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Matrix Metalloproteinase-2 (MMP-2) Fluorometric Drug Discovery Kit, Enzo Life Sciences
Supplier: Enzo Life Sciences
A QuantizymeTM Assay System
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Matrix Metalloproteinase-14 (MMP-14) Fluorometric Drug Discovery Kit, Enzo Life Sciences
Supplier: Enzo Life Sciences
A QuantiZymeTM Assay System
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FLUOR DE LYS® HDAC8 Fluorometric Drug Discovery Kit, Enzo Life Sciences
Supplier: Enzo Life Sciences
A Fluor de Lys® fluorescent assay system
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Matrix Metalloproteinase-7 (MMP-7) Colorimetric Drug Discovery Kit, Enzo Life Sciences
Supplier: Enzo Life Sciences
A QuantiZymeTM Assay SystemThe MMP-7 Colorimetric Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-7 (MMP-7, matrilysin) using a thiopeptide as a chromogenic substrate.
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Amplite® Fluorimetric Beta-Galactosidase Assay Kit *Green Fluorescence* , AAT Bioquest
Supplier: AAT Bioquest
E. coli beta-galactosidase is a 464 Kd tetramer.
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Matrix Metalloproteinase-3 (MMP-3) Colorimetric Drug Discovery Kit, Enzo Life Sciences
Supplier: Enzo Life Sciences
A QuantiZymeTM Assay System
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Matrix Metalloproteinase-1 (MMP-1) Colorimetric Drug Discovery Kit, Enzo Life Sciences
Supplier: Enzo Life Sciences
A QuantiZymeTM Assay System