You searched for: Enzyme Assays

Supplier: Promega Corporation

Recombinant human c-KIT (544-end) was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag. c-KIT is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor).

Supplier: Promega Corporation

Recombinant human EGFR (T790M, L858R) (amino acids 695-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. EGFR is receptor for members of the EGF family and a transmembrane glycoprotein that has tyrosine kinase activity.

Supplier: Promega Corporation

Recombinant human TGF beta R2 (190-end) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. TGF beta R2 is a member of the TGF beta receptor subfamily and is a ser/thr protein kinase.

Supplier: AAT Bioquest

E. coli beta-galactosidase is a 464 kD tetramer.

Supplier: Promega Corporation

Recombinant human FLT3 (D835Y) (571-993) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Supplier: Enzo Life Sciences

An improved Fluor de Lys® HDAC assay with Fluor de Lys®-Green, a new substrate offering higher sensitivity and an excitation and emission (485/530nm) that avoids quenching and fluorescent interference from compounds absorbing in the near UV and blue range.The Fluor de Lys®-Green HDAC assay is a complete kit for measuring histone deacetylase (HDAC) activity in cell or nuclear extracts, immunoprecipitates or purified enzymes

Supplier: AAT Bioquest

Catalase is a common antioxidant heme-containing redox enzyme found in nearly all living organisms that are exposed to oxygen.

Supplier: AAT Bioquest

Alkaline phosphatase is a highly sensitive enzyme for ELISA, immuno-histochemical, Northern, Southern and Western blot applications.

Supplier: AAT Bioquest

Alkaline phosphatase is a highly sensitive enzyme for ELISA, immuno-histochemical, Northern, Southern and Western blot applications.

Supplier: AAT Bioquest

Acetylcholinesterase, also known as AChE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group.

Supplier: Enzo Life Sciences

The MMP inhibitor profiling kit, fluorometric (also known as fluorimetric) RED is a complete assay system designed to examine the specificity of inhibitors against a panel of ten matrix metalloproteinase enzymes, using a quenched fluorogenic substrate OMNIMMP® RED: TQ3-GABA-Pro-Cha-Abu-Smc-His-Ala-Dab(6’-TAMRA)-Ala-Lys-NH2 [TQ3=quencher; GABA=4-aminobutyric acid; Cha=L-cyclohexylalanine; Abu=2-aminobutyric acid; Smc=S-methyl-L-cysteine; Dab=2,4-diaminobutyric acid; 6’-TAMRA=6’-tetramethylrhodamine]. TAMRA fluorescence is thoroughly quenched by the TQ3 group until cleavage by MMPs separates the two moieties.
The assays are performed in a convenient 96-well microplate format. The compound NNGH is also included as a prototypic control inhibitor.

Supplier: Enzo Life Sciences

A QuantiZyme™ Assay System

Supplier: Enzo Life Sciences

A QuantiZymeTM Assay System

Supplier: Enzo Life Sciences

A QuantizymeTM Assay System

Supplier: Enzo Life Sciences

A QuantiZymeTM Assay System

Supplier: Enzo Life Sciences

A Fluor de Lys® fluorescent assay system

Supplier: Enzo Life Sciences

A QuantiZymeTM Assay SystemThe MMP-7 Colorimetric Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-7 (MMP-7, matrilysin) using a thiopeptide as a chromogenic substrate.

Supplier: AAT Bioquest

Histone deacetylases (HDAC) are a class of enzymes that remove acetyl groups from a ε-N-acetyl lysine amino acid on a histone.

Supplier: Enzo Life Sciences

A FLUOR DE LYS® fluorescent assay system

Supplier: ABCAM INC CA

These Serine Protease Assay Kits enable researchers to detect intracellular chymotrypsin-like serine protease activity in vitro without lysing the cell.

Supplier: Enzo Life Sciences

The Cathepsin K Drug Discovery Kit is a complete assay system designed to screen inhibitors of cathepsin K. Using a convenient microplate format, cathepsin K activity is monitored fluorimetrically using the substrate provided in the kit.

Supplier: AAT Bioquest

Glutathione peroxidase is an enzyme family with peroxidase activity to protect the organism from oxidative damage.

Supplier: Enzo Life Sciences

Uses:

Supplier: Rockland Immunochemical

Chemiluminescent Femtomax™ Super Sensitive HRP Substrate For Microwell And/Or Membrane (2 Component System)

Supplier: Enzo Life Sciences

Based on the dye cresyl violet and the cathepsin B ArgArg peptide recognition sequence, this kit allows the detection of cathepsin B activity in living cells by fluorescence microscopy.

Supplier: Enzo Life Sciences

The PTP1B Tyrosine Phosphatase Drug Discovery Kit is a colorimetric, non-radioactive assay designed to measure the phosphatase activity of purified PTP1B. This 96-well assay is useful for screening inhibitors and modulators of PTP1B activity.

Supplier: Promega Corporation

The HDAC-Glo I/II Assays and Screening System are single-reagent-addition, homogeneous, luminescent assays that measure the relative activity of histone deacetylase (HDAC) class I and II enzymes from cells, extracts or purified enzyme sources.

Supplier: AAT Bioquest

Renin is an enzyme that participates in the body's renin-angiotensin system (RAS) that mediates extracellular volume and arterial vasoconstriction.

Supplier: Enzo Life Sciences

It is a complete assay system designed to screen inhibitors of neutrophil elastase, providing enough material to perform at least 96 assays. Neutrophil elastase, also known as leukocyte elastase (LE), is a serine protease found in polymorphonuclear neutrophils (PMNs) that functions in inflammation. It can cleave a variety of proteins, including fibrous elastin, collagen, clotting factors, TNF-α, and α1-antichymotrypsin. It has been shown to be taken up by tumor cells and accelerate tumor growth through its degradation of insulin receptor substrate-1 (IRS-1). Because of its potential roles in cancer and inflammatory diseases such as emphysema, cystic fibrosis, and atherosclerosis, it is a target for drug discovery. The kit uses the chromogenic substrate MeOSuc-Ala-Ala-Pro-Val-pNA; cleavage of p-nitroaniline (pNA) from the substrate increases absorbance at 405nm. A neutrophil elastase inhibitor, elastatinal, is included for use as a control.

Supplier: Promega Corporation

Recombinant human ASK1 (649-946) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. ASK1, also known as MAPKKK5, activates MKK3, MKK4 (SEK1) and MKK6. Overexpression of ASK1 induces apoptotic cell death.