You searched for: Enzyme Assays
Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
ADP-Glo Kinase Assay + c-KIT Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant human c-KIT (544-end) was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag. c-KIT is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor).
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ADP-Glo Kinase Assay + EGFR (T790M, L858R) Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant human EGFR (T790M, L858R) (amino acids 695-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. EGFR is receptor for members of the EGF family and a transmembrane glycoprotein that has tyrosine kinase activity.
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ADP-Glo Kinase Assay + TGF beta R2 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant human TGF beta R2 (190-end) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. TGF beta R2 is a member of the TGF beta receptor subfamily and is a ser/thr protein kinase.
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Amplite® Colorimetric Beta-Galactosidase Assay Kit , AAT Bioquest
Supplier: AAT Bioquest
E. coli beta-galactosidase is a 464 kD tetramer.
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ADP-Glo Kinase Assay + FLT3 (D835Y) Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant human FLT3 (D835Y) (571-993) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
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FLUOR DE LYS®-Green HDAC Fluorometric Activity Assay Kit, Enzo Life Sciences
Supplier: Enzo Life Sciences
An improved Fluor de Lys® HDAC assay with Fluor de Lys®-Green, a new substrate offering higher sensitivity and an excitation and emission (485/530nm) that avoids quenching and fluorescent interference from compounds absorbing in the near UV and blue range.The Fluor de Lys®-Green HDAC assay is a complete kit for measuring histone deacetylase (HDAC) activity in cell or nuclear extracts, immunoprecipitates or purified enzymes
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Amplite® Fluorimetric Catalase Assay Kit *Red Fluorescence* , AAT Bioquest
Supplier: AAT Bioquest
Catalase is a common antioxidant heme-containing redox enzyme found in nearly all living organisms that are exposed to oxygen.
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Amplite® Fluorimetric Alkaline Phosphatase Assay Kit *Blue Fluorescence* , AAT Bioquest
Supplier: AAT Bioquest
Alkaline phosphatase is a highly sensitive enzyme for ELISA, immuno-histochemical, Northern, Southern and Western blot applications.
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Amplite® Luminometric Alkaline Phosphatase Assay Kit *Luminescence* , AAT Bioquest
Supplier: AAT Bioquest
Alkaline phosphatase is a highly sensitive enzyme for ELISA, immuno-histochemical, Northern, Southern and Western blot applications.
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Amplite® Fluorimetric Acetylcholinesterase Assay Kit *Green Fluorescence* , AAT Bioquest
Supplier: AAT Bioquest
Acetylcholinesterase, also known as AChE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group.
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Matrix Metalloproteinase (MMP) Inhibitor Profiling Kit, Fluorometric RED, Enzo Life Sciences
Supplier: Enzo Life Sciences
The MMP inhibitor profiling kit, fluorometric (also known as fluorimetric) RED is a complete assay system designed to examine the specificity of inhibitors against a panel of ten matrix metalloproteinase enzymes, using a quenched fluorogenic substrate OMNIMMP® RED: TQ3-GABA-Pro-Cha-Abu-Smc-His-Ala-Dab(6’-TAMRA)-Ala-Lys-NH2 [TQ3=quencher; GABA=4-aminobutyric acid; Cha=L-cyclohexylalanine; Abu=2-aminobutyric acid; Smc=S-methyl-L-cysteine; Dab=2,4-diaminobutyric acid; 6’-TAMRA=6’-tetramethylrhodamine]. TAMRA fluorescence is thoroughly quenched by the TQ3 group until cleavage by MMPs separates the two moieties.
The assays are performed in a convenient 96-well microplate format. The compound NNGH is also included as a prototypic control inhibitor.
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Cathepsin S Drug Discovery Kit, Enzo Life Sciences
Supplier: Enzo Life Sciences
A QuantiZyme™ Assay System
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Matrix Metalloproteinase-1 (MMP-1) Fluorometric Drug Discovery Kit, Enzo Life Sciences
Supplier: Enzo Life Sciences
A QuantiZymeTM Assay System
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Matrix Metalloproteinase-2 (MMP-2) Fluorometric Drug Discovery Kit, Enzo Life Sciences
Supplier: Enzo Life Sciences
A QuantizymeTM Assay System
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Matrix Metalloproteinase-14 (MMP-14) Fluorometric Drug Discovery Kit, Enzo Life Sciences
Supplier: Enzo Life Sciences
A QuantiZymeTM Assay System
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FLUOR DE LYS® HDAC8 Fluorometric Drug Discovery Kit, Enzo Life Sciences
Supplier: Enzo Life Sciences
A Fluor de Lys® fluorescent assay system
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Matrix Metalloproteinase-7 (MMP-7) Colorimetric Drug Discovery Kit, Enzo Life Sciences
Supplier: Enzo Life Sciences
A QuantiZymeTM Assay SystemThe MMP-7 Colorimetric Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-7 (MMP-7, matrilysin) using a thiopeptide as a chromogenic substrate.
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Amplite® Fluorimetric HDAC Activity Assay Kit *Green Fluorescence* , AAT Bioquest
Supplier: AAT Bioquest
Histone deacetylases (HDAC) are a class of enzymes that remove acetyl groups from a ε-N-acetyl lysine amino acid on a histone.
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FLUOR DE LYS® SIRT3 Fluorometric Drug Discovery Assay Kit, Enzo Life Sciences
Supplier: Enzo Life Sciences
A FLUOR DE LYS® fluorescent assay system
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Serine Protease Assay Kit (FAM-Phe-DAP), Abcam
Supplier: ABCAM INC CA
These Serine Protease Assay Kits enable researchers to detect intracellular chymotrypsin-like serine protease activity in vitro without lysing the cell.
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Cathepsin K Drug Discovery Kit, Enzo Life Sciences
Supplier: Enzo Life Sciences
The Cathepsin K Drug Discovery Kit is a complete assay system designed to screen inhibitors of cathepsin K. Using a convenient microplate format, cathepsin K activity is monitored fluorimetrically using the substrate provided in the kit.
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Amplite® Fluorimetric Glutathione Peroxidase Assay Kit *Blue Fluorescence* , AAT Bioquest
Supplier: AAT Bioquest
Glutathione peroxidase is an enzyme family with peroxidase activity to protect the organism from oxidative damage.
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Ubiquitin Activating Kit, Enzo Life Sciences
Supplier: Enzo Life Sciences
Uses:
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Chemiluminescent Femtomax™ Super Sensitive HRP Substrate For Microwell And/Or Membrane (2 Component System), Rockland Immunochemicals
Supplier: Rockland Immunochemical
Chemiluminescent Femtomax™ Super Sensitive HRP Substrate For Microwell And/Or Membrane (2 Component System)
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CV-Cathepsin B Detection Kit, Enzo Life Sciences
Supplier: Enzo Life Sciences
Based on the dye cresyl violet and the cathepsin B ArgArg peptide recognition sequence, this kit allows the detection of cathepsin B activity in living cells by fluorescence microscopy.
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PTP1B Drug Discovery Kit, Enzo Life Sciences
Supplier: Enzo Life Sciences
The PTP1B Tyrosine Phosphatase Drug Discovery Kit is a colorimetric, non-radioactive assay designed to measure the phosphatase activity of purified PTP1B. This 96-well assay is useful for screening inhibitors and modulators of PTP1B activity.
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HDAC-Glo I/II Assay, Promega
Supplier: Promega Corporation
The HDAC-Glo I/II Assays and Screening System are single-reagent-addition, homogeneous, luminescent assays that measure the relative activity of histone deacetylase (HDAC) class I and II enzymes from cells, extracts or purified enzyme sources.
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Amplite® Fluorimetric Renin Assay Kit *Red Fluorescence* , AAT Bioquest
Supplier: AAT Bioquest
Renin is an enzyme that participates in the body's renin-angiotensin system (RAS) that mediates extracellular volume and arterial vasoconstriction.
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Neutrophil elastase colorimetric drug discovery kit, Enzo Life Sciences
Supplier: Enzo Life Sciences
It is a complete assay system designed to screen inhibitors of neutrophil elastase, providing enough material to perform at least 96 assays. Neutrophil elastase, also known as leukocyte elastase (LE), is a serine protease found in polymorphonuclear neutrophils (PMNs) that functions in inflammation. It can cleave a variety of proteins, including fibrous elastin, collagen, clotting factors, TNF-α, and α1-antichymotrypsin. It has been shown to be taken up by tumor cells and accelerate tumor growth through its degradation of insulin receptor substrate-1 (IRS-1). Because of its potential roles in cancer and inflammatory diseases such as emphysema, cystic fibrosis, and atherosclerosis, it is a target for drug discovery. The kit uses the chromogenic substrate MeOSuc-Ala-Ala-Pro-Val-pNA; cleavage of p-nitroaniline (pNA) from the substrate increases absorbance at 405nm. A neutrophil elastase inhibitor, elastatinal, is included for use as a control.
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ADP-Glo Kinase Assay + ASK1 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant human ASK1 (649-946) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. ASK1, also known as MAPKKK5, activates MKK3, MKK4 (SEK1) and MKK6. Overexpression of ASK1 induces apoptotic cell death.