Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
ADP-Glo Kinase Assay + PDGFR beta Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant human PDGFR beta (amino acids 557-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PDGFR beta is a member of the PDGFR family of membrane receptors with intrinsic tyrosine kinase activity.
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ADP-Glo Kinase Assay + GSK3 beta Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human GSK3 beta was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. GSK3 beta is a serine-threonine protein kinase originally identified as the kinase that phosphorylates and inhibits glycogen synthase.
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ADP-Glo Kinase Assay + CDK1/CyclinA2 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human CDK1 and CyclinA2 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. CDK1 is essential for completion of START, controlling event in the cell cycle required to initiate mitosis.
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ADP-Glo Kinase Assay + CSK Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human CSK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CSK has been shown to downregulate the tyrosine kinase activity of c-src through tyrosine phosphorylation of the c-src carboxy terminus.
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ADP-Glo Kinase Assay + FLT1 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant human FLT1 (amino acids 784-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. FLT1, also known as fms-like tyrosine kinase 1, is a member of the VEGFR family and is related to the oncogene ROS.
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ADP-Glo Kinase Assay + PKC beta II Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human PKC beta II was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PKC beta II is a member of the protein kinase C (PKC) family of serine- and threonine-specific protein kinases.
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ADP-Glo Kinase Assay + ITK Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant human ITK (amino acids 352-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. ITK is a member of the TEC family of nonreceptor tyrosine kinases.
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ADP-Glo Kinase Assay + RET Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant human RET (amino acids 658-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. RET gene codes for a transmembrane tyrosine kinase.
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ADP-Glo Kinase Assay + InsR Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant human InsR (amino acids 1011-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. InsR is the insulin receptor tyrosine kinase that is involved in insulin signaling.
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ADP-Glo Kinase Assay + FYN A Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Full-length recombinant human FYN A was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. FYN A (Fyn isoform A) is a member of the SRC tyrosine kinase oncogene family showing high homology to YES1, FGR and SRC.
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ADP-Glo Kinase Assay + SRC Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human SRC was expressed in E. coli cells using an N-terminal GST tag. SRC was originally identified as a transforming protein of the Rous sarcoma virus that had enzymatic ability to phosphorylate tyrosine in protein substrates.
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ADP-Glo Kinase Assay + LYN B Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human LYN B was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. LYN is a 56kDa tyrosine kinase that is similar to mouse T-lymphocyte-specific tyrosine kinase p56lck, v-yes, v-fgr and v-src.
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ADP-Glo Kinase Assay + AXL Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant human AXL (amino acids 473-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. AXL is a member of the receptor tyrosine kinase family, which has oncogenic potential and is implicated in human myeloid leukemia.
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ADP-Glo Kinase Assay + CDK2/CyclinA2 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human CDK2 and CyclinA2 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. CDK2 is a member of the Cyclin-Dependent Kinase family that is ubiquitously expressed.
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ADP-Glo Kinase Assay + PKC zeta Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human PKC zeta was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PKC zeta is an atypical isoform of the PKC family.
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ADP-Glo Kinase Assay + PKC delta Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human PKC delta was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Protein kinase C delta is a member of the protein kinase C (PKC) family of serine-threonine kinases.
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ADP-Glo Kinase Assay + SYK Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human SYK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. SYK is a non-receptor protein tyrosine kinase that is widely expressed in hematopoietic cells.
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ADP-Glo Kinase Assay + FAK Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant human FAK was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. FAK is involved in signal transduction from integrin-enriched focal adhesion sites that mediate cell contact with the extracellular matrix.
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ADP-Glo Kinase Assay + EGFR Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant human EGFR (amino acids 695-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. EGFR is the receptor for members of the EGF family and is a transmembrane glycoprotein that has tyrosine kinase activity.
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ADP-Glo Kinase Assay + RSK2 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human RSK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. RSK2 is a member of the RSK (ribosomal S6 kinase) family that are growth factor-regulated serine-threonine kinases.
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ADP-Glo Kinase Assay + HER2 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant human HER2 (amino acids 676-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. HER2 gene encodes a cell-surface glycoprotein tyrosine kinase receptor with extensive homology to epidermal growth factor receptor.
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ADP-Glo Kinase Assay + PDK1 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human PDK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. PDK1 (3-phosphoinositide-dependent protein kinase) is activated by the presence of PtdIns(3,4,5)P3 or PtdIns(3,4)P2.
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Phospholipase D, Streptomyces chromofuscus, Enzyme Assay, MilliporeSigma
Supplier: MilliporeSigma
Native phospholipase D from Streptomyces chromofuscus
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ADP-Glo Kinase Assay + PDGFR alpha Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant human PDGFR alpha (amino acids 550-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PDGFR alpha is a member of the PDGFR family of membrane receptors with intrinsic tyrosine kinase activity.
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ADP-Glo Kinase Assay + PKC alpha Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human PKC alpha was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PKC alpha is a member of the protein kinase C (PKC) family of serine- and threonine-specific protein kinases.
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ADP-Glo Kinase Assay + GSK3 alpha Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human GSK3 alpha was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. GSK3 alpha is a multifunctional protein serine kinase implicated in the control of several regulatory proteins.
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SAM510™ Colorimetric, Continuous Enzyme Coupled SAM Methyltransferase Assay, G-Biosciences
Supplier: G-Biosciences
Methylation of key biological molecules and proteins plays important roles in numerous biological systems, including signal transduction, biosynthesis, protein repair, gene silencing and chromatin regulation
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ADP-Glo Kinase Assay + AMPK (A1/B1/G1) Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human AMPK (combination of A1/B1/G1 subunits) was expressed by baculovirus in Sf9 insect cells using C-terminal His tags. AMPK is an important energy-sensing enzyme that monitors cellular energy status.
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SAMfluoro™ Fluorescent, Continuous Enzyme Coupled SAM Methyltransferase Assay, G-Biosciences
Supplier: G-Biosciences
Methylation of key biological molecules and proteins plays important roles in numerous biological systems, including signal transduction, biosynthesis, protein repair, gene silencing and chromatin regulation
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ADP-Glo Kinase Assay + PLK1 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human PLK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. A member of the Polo-Like Kinase family, PLK1 localizes to centrosomes and undergoes dramatic subcellular relocation during the cell cycle.