You searched for: Enzyme Assays
Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
Beta-Galactosidase Enzyme Assay System with Reporter Lysis Buffer, 10 ml, Promega
Supplier: Promega Corporation
The Beta-Galactosidase Enzyme Assay System with Reporter Lysis Buffer is used to assay beta-galactosidase activity in cell lysates.
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ADP-Glo Kinase Assay + FLT3 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
FLT3 is a receptor tyrosine kinase that has been shown to play a role in proliferation and survival of hematopoietic progenitor cells as well as differentiation of early B lymphoid progenitors.
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ADP-Glo Kinase Assay + BRK Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human BRK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. BRK is a member of the non-receptor tyrosine kinases (PTKs) that contains an amino terminal SH3 and SH2 domain as well as the catalytic domain.
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ADP-Glo Kinase Assay + KHS1 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human KHS1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. KHS1 belongs to the ser/thr kinase family that has a STE20-like protein kinase domain, which stimulates the SAPK pathway.
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ADP-Glo Kinase Assay + CAMKK1 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
CAMKK1 or CAMKK alpha is a Ca2+/calmodulin-dependent protein kinase that activates CAM-kinases I and IV via phosphorylation of their Thr(177) and Thr(196) residues, respectively.
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ADP-Glo Kinase Assay + PIM2 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human PIM2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PIM2 is a serine threonine kinase that is present in all tissues.
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ADP-Glo Kinase Assay + PKD2 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human PKD2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PKD2 is a novel phorbol ester- and growth factor-stimulated serine/threonine kinase.
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ADP-Glo Kinase Assay + HIPK1 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant human HIPK1 (156-555) was expressed in Sf9 cells using an N-terminal GST tag. HIPK1 or homeodomain-interacting protein kinase 1 is a ser/thr protein kinase and a member of the HIPK family.
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ADP-Glo Kinase Assay + FMS Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant human FMS (539-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. FMS is a proto-oncogene that encodes the tyrosine kinase transmembrane receptor for colony stimulating factor 1 receptor (CSF1R).
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ADP-Glo Kinase Assay + CLK1 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant human CLK1 (129-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CLK1, also known as STY, is a member of the CDC2-like (or LAMMER) family of dual specificity protein kinases.
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ADP-Glo Kinase Assay + JNK1 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human JNK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. JNK1 is a member of the MAP kinase group that is activated by dual phosphorylation at the thr and tyr residues during exposure to stress.
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ADP-Glo Kinase Assay + MST1 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human MST1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MST1 belongs to a family of proteins that share similarity with a budding yeast serine/threonine kinase, sterile-20 (Ste20).
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ADP-Glo Kinase Assay + PAK3 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length mouse PAK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PAK3 is a member of the family of ser/thr p21-activating kinases that serve as targets for the small GTP-binding proteins Cdc42 and RAC.
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ADP-Glo Kinase Assay + ACK Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
ACK is a tyrosine kinase that binds CDC42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of CDC42Hs.
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ADP-Glo Kinase Assay + PYK2 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant human PYK2 (360-690) was expressed by baculovirus in Sf9 cells using an N-terminal His tag. PYK2 (also known as FAK2/RAFTK) is a member of the focal adhesion PTK family.
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ADP-Glo Kinase Assay + SLK Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human SLK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. SLK or LOSK is a Ste20-like protein kinase that associates with microtubule and centrosome.
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ADP-Glo Kinase Assay + MAPKAPK5 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human MAPKAPK5 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MAPKAPK5 is a member of the serine/threonine kinase family that responds to cellular stress and proinflammatory cytokines.
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ADP-Glo Kinase Assay + FGFR4 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant human FGFR4 (460-end) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. FGFR4 is a member of the fibroblast growth factor receptor family, which play a role in mitogenesis and differentiation.
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ADP-Glo Kinase Assay+ MAPKAPK3 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human MAPKAPK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MAPKAPK3 is closely related to MAPKAPK2 sharing 72% nucleotide and 75% amino acid identity.
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ADP-Glo Kinase Assay + FGFR2 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant human FGFR2 (285-end) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. FGFR2 is a member of the fibroblast growth factor receptor family, which play a role in mitogenesis and differentiation.
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ADP-Glo Kinase Assay + LRRK2 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
LRRK2 or leucine-rich repeat kinase is a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain and a WD40 domain.
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ADP-Glo Kinase Assay + MLK1 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant human MLK1 (1-433) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Mixed-Lineage Kinase 1 is a mitogen-activated protein kinase kinase kinase capable of activating the c-Jun NH(2)-terminal kinase (JNK) pathway.
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ADP-Glo Kinase Assay + PKC epsilon Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human PKC epsilon was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PKC epsilon is a member of the protein kinase C (PKC) family of serine- and threonine-specific protein kinases.
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ADP-Glo Kinase Assay + MARK1 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human MARK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MARK1 is a member of the MARK family and is a serine/threonine-protein kinase that plays a key role in signal transduction.
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ADP-Glo Kinase Assay + CAMK2 alpha Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human CAMK2 alpha was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. CAMK2 alpha is a ser/thr protein kinase that is a member of the Ca2+/calmodulin-dependent protein kinase family.
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ADP-Glo Kinase Assay + CHK2 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human CHK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CHK2 is rapidly phosphorylated and activated in response to replication blocks and DNA damage.
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ADP-Glo Kinase Assay + DAPK1 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant human DAPK1 (1-363) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Death-associated protein kinase 1 (DAPK1) is a positive mediator of apoptosis induced by gamma-interferon.
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ADP-Glo Kinase Assay + AKT3 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human AKT3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. AKT3 or Protein Kinase B gamma (PKBgamma) is a serine/threonine kinase that is a member of the AKT family.
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ADP-Glo Kinase Assay + RSK1 Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human RSK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. RSK1 is a member of the RSK (ribosomal S6 kinase) family that consists of growth factor-regulated serine/threonine kinases.
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ADP-Glo Kinase Assay + TOPK Kinase Enzyme System, 1 each, Promega
Supplier: Promega Corporation
Recombinant full-length human TOPK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. TOPK is a MAPK kinase that phosphorylates p38 MAPK and is activated in a cell-cycle-dependent manner in neuronal progenitor cells in vitro.