Enzyme Assays
Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
Matrix Metalloproteinase-8 (MMP-8) Colorimetric Drug Discovery Kit, Enzo Life Sciences
Supplier: Enzo Life Sciences
A QuantiZymeTM Assay System
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Pierce™ β-Galactosidase Assay Kits, Thermo Scientific
Supplier: Thermo Scientific
Detect more β-galactosidase activity by lysing the cells efficiently.
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Akt kinase activity kit, Enzo Life Sciences
Supplier: Enzo Life Sciences
For the measurement of Akt in partially purified, purified, or crude enzyme preparations from any species.
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Pierce™ Kinase Enrichment Kit, Thermo Scientific
Supplier: Thermo Scientific
The Pierce™ Kinase Enrichment Kits utilise an ActivX™ ATP or ADP probe to covalently label the active site of ATPases, including chaperones and metabolic enzymes, to enable their selective enrichment using a desthiobiotin tag.
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DLG (Dilauroyl-sn-glycerol)
Supplier: Sino Biological
The substrate solution used for assaying Diacylglycerol kinases.
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Cathepsin K Drug Discovery Kit, Enzo Life Sciences
Supplier: Enzo Life Sciences
The Cathepsin K Drug Discovery Kit is a complete assay system designed to screen inhibitors of cathepsin K. Using a convenient microplate format, cathepsin K activity is monitored fluorimetrically using the substrate provided in the kit.
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PTP1B Drug Discovery Kit, Enzo Life Sciences
Supplier: Enzo Life Sciences
The PTP1B Tyrosine Phosphatase Drug Discovery Kit is a colorimetric, non-radioactive assay designed to measure the phosphatase activity of purified PTP1B. This 96-well assay is useful for screening inhibitors and modulators of PTP1B activity.
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CV-Cathepsin B Detection Kit, Enzo Life Sciences
Supplier: Enzo Life Sciences
Based on the dye cresyl violet and the cathepsin B ArgArg peptide recognition sequence, this kit allows the detection of cathepsin B activity in living cells by fluorescence microscopy.
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FLUOR DE LYS® SIRT3 Fluorometric Drug Discovery Assay Kit, Enzo Life Sciences
Supplier: Enzo Life Sciences
A FLUOR DE LYS® fluorescent assay system
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MMP Inhibitor Profiling Kit, Fluorometric, Enzo Life Sciences
Supplier: Enzo Life Sciences
A QUANTIZYME® Assay System Complete assay system designed to examine the specificity of inhibitors against a panel of ten matrix metalloproteinase enzymes. The assays are performed in a convenient 96-well microplate format using a sensitive fluorogenic substrate . Included are active MMP enzymes, assay buffer, a prototypic control inhibitor (NNGH3), and a detailed instruction booklet.
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Serine Protease Assay Kit (FAM-Phe-DAP), Abcam
Supplier: Abcam
These Serine Protease Assay Kits enable researchers to detect intracellular chymotrypsin-like serine protease activity in vitro without lysing the cell.
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Ubiquitin Activating Kit, Enzo Life Sciences
Supplier: Enzo Life Sciences
Uses:
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Ca²⁺/Calmodulin Solution II, 10X
Supplier: Sino Biological
10X stock solution used for assaying activity of calcium-calmodulin-activated protein kinase enzymes.
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Neutrophil elastase colorimetric drug discovery kit, Enzo Life Sciences
Supplier: Enzo Life Sciences
It is a complete assay system designed to screen inhibitors of neutrophil elastase, providing enough material to perform at least 96 assays. Neutrophil elastase, also known as leukocyte elastase (LE), is a serine protease found in polymorphonuclear neutrophils (PMNs) that functions in inflammation. It can cleave a variety of proteins, including fibrous elastin, collagen, clotting factors, TNF-α, and α1-antichymotrypsin. It has been shown to be taken up by tumor cells and accelerate tumor growth through its degradation of insulin receptor substrate-1 (IRS-1). Because of its potential roles in cancer and inflammatory diseases such as emphysema, cystic fibrosis, and atherosclerosis, it is a target for drug discovery. The kit uses the chromogenic substrate MeOSuc-Ala-Ala-Pro-Val-pNA; cleavage of p-nitroaniline (pNA) from the substrate increases absorbance at 405nm. A neutrophil elastase inhibitor, elastatinal, is included for use as a control.
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Complex IV Rodent Enzyme Activity Dipstick Assay Kit
Supplier: Abcam
Complex IV Rodent Enzyme Activity Dipstick Assay Kit is an immunoassay kit for the detection of Complex IV Rodent Enzyme Activity in Mouse, Rat in Cell culture extracts, Tissue samples.
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Serine Protease Assay Kit (FAM-Spacer-Leu-CMK), Abcam
Supplier: Abcam
These Serine Protease Assay Kits enable researchers to detect intracellular chymotrypsin-like serine protease activity in vitro without lysing the cell.
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