You searched for: Protein Linking and Labeling Systems
Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.
AnaTag™ HiLyte™ Fluor 750 Microscale Protein Labeling Kit *Ultra Convenient*, Anaspec
Supplier: Anaspec Inc
HiLyte™ Fluor 750 acid, SE is the longest-wavelength amine-reactive HiLyte™ Fluor dye currently available. Its fluorescence emission maximum at 778 nm is well separated from commonly used far-red fluorophores such as HiLyte™ Fluor 647, HiLyte™ Fluor 680 or allophycocyanin (APC), facilitating multicolor analysis.
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Pierce™ Fluor Phosphine Reagents, DyLight™, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific DyLight Fluor Phosphine Reagents are phosphine-activated fluorescent dyes for specific labeling and detection of azide-tagged molecules, which enables use of fluorescence imaging in metabolic labeling strategies.
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Pierce™ EZ-Link™ Plus Activated Peroxidase, Thermo Scientific
Supplier: Invitrogen
Activated peroxidase is an amine-reactive form of horseradish peroxidase (HRP) that provides coupling efficiencies of greater than 95% with antibodies and other proteins.
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Pierce™ EZ-Link® Sulfhydryl Reactive Biotinylation Reagents, Thermo Scientific
Supplier: Invitrogen
Sulfhydryl groups are the second most common target for modification in biological studies. They are found as exposed cysteines in protein and peptides. Sulfhydryl group biotinylation provides an advantage in some applications; for example, targeting a sulfhydryl group can help preserve the biological activity of an enzyme when amines are found at the active site.
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LysoBrite™ Lysosome Dye, Red DND-99
Supplier: STEMCELL Technologies
Fluorescent dyes used to label and track lysosomes in live cells - 500 tests.
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Pierce™ Magnetic RNA-Protein Pull-Down Kit, Thermo Scientific
Supplier: Invitrogen
The Thermo Scientific Pierce Magnetic RNA-Protein Pull-Down Kit provides researchers with a streamlined, robust method to enrich protein-RNA interactions using end-labeled RNA as the bait.
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Pierce™ CA(PEG)n Carboxy-PEG-Amine Compound, Thermo Scientific
Supplier: Invitrogen
The carboxy-PEGn-amine (CA[PEG]n) PEGylation reagents are zwitterionic, amino acid derivatives that are used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers
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Pierce™ Sulfo-SBED Biotin Label Transfer Kit and Reagents - Western Blot Application, Thermo Scientific
Supplier: Invitrogen
Sulfo-SBED is a trifunctional crosslinking reagent containing a biotin, a sulfonated N-hydroxysuccinimide (Sulfo-NHS) active ester and a photoactivatable aryl azide
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Pierce™ Dye Removal Columns, Thermo Scientific
Supplier: Invitrogen
Pierce™ Dye Removal Columns enable fast and efficient removal of non-reacted fluorescent dyes from protein labeling reactions.
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Pierce™ Immunoprecipitation, Crosslink IP Kit, Thermo Scientific
Supplier: Invitrogen
The Pierce™ Crosslink Immunoprecipitation (IP) Kit enables highly effective and efficient antigen immunoprecipitations by covalently crosslinking antibodies onto Protein A/G resin.
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Pierce™ Azide Labeled Sugars, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Pierce™ Azide-Labeled sugars provide a highly specific approach for studying glycoproteins through in vivo metabolic labeling and chemoselective ligation.
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PhosphoBlocker™ Blocking Reagent, Cell Biolabs
Supplier: Cell Biolabs
PhosphoBLOCKER™ Blocking Reagent provides superior blocking by maximizing signal-to-noise ratio
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Biotin Conjugate Collagen Hybridizing Peptide (Biotin), Advanced Biomatrix
Supplier: ADVANCED BIOMATRIX, INC. MS
CHP can specifically bind to denatured collagen.
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Pierce™ Sulfhydryl Addition Kit, Thermo Scientific
Supplier: Invitrogen
Introduce free sulfhydryl groups into a protein of interest to allow the modified protein to react with a variety of sulfhydryl-selective products.
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AnaTag™ HiLyte™ Fluor 555 Microscale Protein Labeling Kit *Ultra Convenient*, Anaspec
Supplier: Anaspec Inc
HiLyte™ Fluor 555 acid, SE is an excellent amine-reactive fluorescent labeling dye that generates the protein conjugates that are only slightly red-shifted compared to those of Cy™ 3 dyes, resulting in an optimal match to filters designed for CyTM dyes.
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Pierce™ Controlled Protein-Protein Cross-Linking Kit, Thermo Scientific
Supplier: Invitrogen
The Pierce™ Controlled Protein-Protein Crosslinking Kit contains essential crosslinkers, modification reagents and buffers for conjugating antibodies, enzymes and other purified proteins needed as probes.They insert an unbreakable molecular bridge between two proteins with easy-to-control stepwise coupling.
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AnaTag™ 5 - FITC Microscale Protein Labeling Kit *Ultra Convenient*, Anaspec
Supplier: Anaspec Inc
Fluorescein isothiocyanate (FITC) remains one of the most popular fluorescent labeling reagents, probably due to the low cost of the material. 5-FITC and 6-FITC have very similar absorption and fluorescence spectra. However, the isomers may differ in their binding and reactivity to proteins, and the conjugates may elute under different chromatographic conditions or migrate differently in electrophoretic gels.
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AnaTag™ AMCA - X Microscale Protein Labeling Kit *Ultra Convenient*, Anaspec Inc.
Supplier: Anaspec Inc
AMCA-X, SE is one of the most popular blue fluorescent tagging molecules. It is widely used to label antibodies, proteins and small drug molecules. AMCA-X succinimidyl ester contains a seven-atom aminohexanoyl spacer between the fluorophore and the reactive group.
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Pierce™ RNA 3' End Biotinylation Kit, Thermo Scientific
Supplier: Invitrogen
The Thermo Scientific Pierce RNA 3' End Biotinylation Kit is optimized for labeling the 3-prime terminal end of RNA probes to facilitate their use as probes or targets in EMSA and other methods for studying protein-RNA interactions.
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Pierce™ Immobilized Anti-TMT™ Antibody Resin, Thermo Scientific
Supplier: Invitrogen
Anti-TMT™ Antibody resin enables selective enrichment of peptides in complex samples that have been labeled with Tandem Mass Tag™ Reagents.
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5-FAM Conjugate Collagen Hybridizing Peptide (Green), Advanced Biomatrix
Supplier: ADVANCED BIOMATRIX, INC. MS
CHP can specifically bind to denatured collagen.
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AnaTag™ 5 - FITC Protein Labeling Kit *Ultra Convenient*, Anaspec
Supplier: Anaspec Inc
Fluorescein isothiocyanate (FITC) remains one of the most popular fluorescent labeling reagents, probably due to the low cost of the material. 5-FITC and 6-FITC have very similar absorption and fluorescence spectra. However, the isomers may differ in their binding and reactivity to proteins, and the conjugates may elute under different chromatographic conditions or migrate differently in electrophoretic gels.
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Pierce™ DyLight™ Antibody Labelling Dyes, Thermo Scientific
Supplier: Invitrogen
DyLight™ Dyes have absorption spectra ranging from 350 nm to 770 nm and match the output wavelengths of common fluorescence instrumentation
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Fluorescein Labeled Lycopersicon Esculentum Lectin (LEL), Vector Laboratories
Supplier: Vector Laboratories
Fluorescein labeled Lycopersicon Esculentum (Tomato) Lectin (LEL, TL). Tomato lectin is a very stable single subunit glycoprotein containing about 50 percent arabinose and galactose and may form multimeric aggregates in solution. LEL binds well to glycophorin and Tamm-Horsfall glycoprotein and has been used effectively to label vascular endothelium in rodents.
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AnaTag™ HiLyte™ Fluor 488 Microscale Protein Labeling Kit, Anaspec
Supplier: Anaspec Inc
HiLyte™ Fluor 488, SE is an excellent amine-reactive fluorescent labeling dye for generating protein conjugates. The spectrum of HiLyte™ Fluor488 is similar to fluorescein (FITC), resulting in an optimal match to filters designed for fluorescein.
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Pierce™ EZ-Link™ Amine Reactive Biotinylation Reagents, Thermo Scientific
Supplier: Invitrogen
The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.
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BirA Biotin-Protein Ligase Reaction Kits, Avidity
Supplier: AVIDITY LLC MS
The BirA biotin-protein ligase adds d-biotin covalently to biotin-acceptor peptides/proteins via an ATP intermediate (biotinyl 5'-adenylate) in a highly efficient and targeted manner.
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Pierce™ RNA 3' End Desthiobiotinylation Kit, Thermo Scientific
Supplier: Invitrogen
The Thermo Scientific Pierce RNA 3'-End Desthiobiotinylation Kit contains reagents for easy and efficient desthiobiotin labeling of RNA for use as probes or as antibody alternatives for enrichment of RNA binding proteins.
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Pierce™ DyLight™ Antibody Labeling, NHS Ester Quenchers, Thermo Scientific
Supplier: Invitrogen
DyLight™ NHS Ester Quenchers are amine-reactive non-fluorescent dyes that use fluorescent resonance energy transfer (FRET) to quench fluorescent signals resulting from ultraviolet, visible and infrared light-excited fluorescent dyes
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StabilZyme® Protein-Free AP Stabilizer, Surmodics
Supplier: Surmodics
StabilZyme® AP and StabilZyme® Protein-Free AP stabilizers provide assay developers a complete set of tools to stabilize different AP conjugates for long term, in-solution stability.