Protein Linking and Labeling Systems
Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.
Pierce™ Immobilized Anti-TMT™ Antibody Resin, Thermo Scientific
Supplier: Invitrogen
Anti-TMT™ Antibody resin enables selective enrichment of peptides in complex samples that have been labeled with Tandem Mass Tag™ Reagents.
Expand 1 Items
Pierce™ Azide Labeled Sugars, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Pierce™ Azide-Labeled sugars provide a highly specific approach for studying glycoproteins through in vivo metabolic labeling and chemoselective ligation.
Expand 3 Items
Pierce™ PEGylated SPDP Crosslinkers, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Pierce™ PEGn-SPDP is a multifunctional crosslinker for protein conjugation via amine-to-amine or amine-to-sulfhydryl crosslinks that contain a n-unit polyethylene glycol (PEG) group and a reducible (cleavable) disulfide bond.
Expand 2 Items
Pierce™ Antibody Biotinylation Kit for IP, Thermo Scientific
Supplier: Invitrogen
The Thermo Scientific™ Pierce™ Antibody Biotinylation Kit for IP provides biotinylation reagents designed specifically for the labeling of primary antibodies used in immunoprecipitation applications.Optimized for IP—reagents and protocols developed specifically to address antibody labeling for immunoprecipitation applicationsEasy to use—kit configuration specifically optimized to accommodate 50–200 µg of antibody in a 100 µL reaction; desalting columns are provided to remove excess biotinylation reagent after labelingAmine reactive—reacts with primary amines (-NH2) of antibodiesEnhanced solubility—pegylation improves water solubility of the biotinylated antibody and prevents aggregation upon storageReduced steric hindrance—longer spacer arm (29 angstroms) minimize steric hindrance when binding to avidin moleculesThe Pierce Antibody Biotinylation Kit reagents have been optimized and validated to biotin label antibodies for IP and co-IP reactions
Expand 1 Items
AnaTag™ Biotin Protein Labeling Kit, Anaspec Inc.
Supplier: Anaspec
Biotin, SE is amino-reactive biotinylating reagent for peptides and proteins; it has a better avidin-binding affinity than biotin. Biotin, SE (d-Biotin-N-hydroxysuccinimide ester) contains a five-carbon spacer arm that reduces steric hindrance associated with binding four biotinylated molecules per one avidin and results in enhanced detection sensitivity.
Expand 1 Items
AnaTag™ 5 - TAMRA Microscale Protein Labeling Kit *Ultra Convenient*, Anaspec
Supplier: Anaspec
TAMRA is one of the most popular fluorophores used in various bioconjugations. 5-TAMRA is the purified single isomer of 5(6)-TAMRA. It is preferred for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between 5-TAMRA and 6-TAMRA might affect some biological properties of the underlying conjugates.
Expand 1 Items
Fluorescent Dyes, M series, Streptavidin Conjugate
Supplier: Abnova
Fluorescent dye, M series, products are designed to be maximally excited by one of the major light sources equipped in flow cytometers. In comparison with phycobiliprotein tandems, fluorescent dye, M series, possesses better photostability, higher conjugation yields, and little pH sensitivity making it an ideal choice in fluorescence imaging applications.
Expand 2 Items
LysoBrite™ Lysosome Dye, Red DND-99
Supplier: Stemcell Technologies
Fluorescent dyes used to label and track lysosomes in live cells - 500 tests.
Expand 1 Items
ReadiLink™ Rapid Cy5 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* (2 Labelings), AAT Bioquest
Supplier: AAT Bioquest
Cy5 is one of the most popular fluorescent labeling dyes for preparing orange-red fluorescent bioconjugates.
Expand 1 Items
Buccutite™ Rapid Protein Crosslinking Kit *Microscale Optimized for Crosslinking 100 ug Antibody Per Reaction* (2 Labelings), AAT Bioquest
Supplier: AAT Bioquest
Protein-protein conjugations are commonly performed with a bifunctional linker such as SMCC.
Expand 1 Items
ReadiLink™ KLH Conjugation Kit (2 Reactions), AAT Bioquest
Supplier: AAT Bioquest
Keyhole Limpet Hemocyanin (KLH) is one of the most commonly used carriers in the conjugation of peptides for antibody production.
Expand 1 Items
ReadiLink™ Rapid iFluor® 555 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* (2 Labelings), AAT Bioquest
Supplier: AAT Bioquest
AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies.
Expand 1 Items
ReadiLink™ Rapid FITC Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* (2 Labelings), AAT Bioquest
Supplier: AAT Bioquest
FITC is still the most popular fluorescent labeling dye for preparing green fluorescent bioconjugates.
Expand 1 Items
ReadiLink™ Protein Biotinylation Kit *Powered by ReadiView™ Biotin Visionization Technology* (3 Reactions), AAT Bioquest
Supplier: AAT Bioquest
Biotin is widely used for labeling biomolecules, in particular, antibodies.
Expand 1 Items
ReadiLink™ xtra Rapid XFD488 Antibody Labeling Kit *BSA-Compatible, XFD488 Same Structure to Alexa Fluor™ 488* (2 Labelings), AAT Bioquest
Supplier: AAT Bioquest
XFD488 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 488 (Alexa Fluor® is the trademark of ThermoFisher).