Protein Linking and Labeling Systems

Supplier: Bulldog Bio

Novel Antibody Fragment that binds GFP Fusion Proteins.

Supplier: Anaspec

AMCA-X, SE is one of the most popular blue fluorescent tagging molecules. It is widely used to label antibodies, proteins and small drug molecules. AMCA-X succinimidyl ester contains a seven-atom aminohexanoyl spacer between the fluorophore and the reactive group.

Supplier: Invitrogen

Cross linkers are used for the identification of near-neighbor protein relationships and ligand-receptor interactions.

Supplier: Invitrogen

Eliminate soluble components and reduce disulfide bonds without sacrificing valuable protein or peptide samples.

Supplier: List Biological Laboratories, Inc.

The potent toxicity of both the botulinum neurotoxins and anthrax lethal toxin is due to a zinc-dependent proteolytic activity associated with the toxins.  Measurement of this enzymatic activity provides for both a potentially sensitive and direct means for detection of the toxin, and a method for identifying potential toxin inhibitors using high throughput screening. A highly efficient approach for monitoring enzymatic activity is based on the use of fluorescence resonance energy transfer (FRET) substrates. These fluorogenic peptides contain a donor fluorescent group at one end and a suitable chromogenic acceptor group at the other.  The fluorescence is quenched initially by intramolecular energy transfer between the donor/acceptor pair.  Cleavage of the FRET substrate by the appropriate enzyme releases the fluorophore and full fluorescence is restored.  The increase in fluorescence intensity is directly proportional to the amount of enzyme present.  Enzymatic activity can be monitored continuously by recording the increase in fluorescence intensity with time.  The change in the relative fluorescence units (RFU) as cleavage occurs can be converted to nmoles of cleaved substrate from a standard curve generated using a Calibration Peptide which is the cleaved substrate containing only the N-terminally attached fluorophore.  For Botulinum neurotoxin type A, a Control FRET peptide substrate that is not cleaved by the neurotoxin but contains all remaining non-specific sites in the sequence can be used to screen background cleavage of the substrate that can occur in complex matrices.

Supplier: Abnova

Fluorescent dye, M series, products are designed to be maximally excited by one of the major light sources equipped in flow cytometers. In comparison with phycobiliprotein tandems, fluorescent dye, M series, possesses better photostability, higher conjugation yields, and little pH sensitivity making it an ideal choice in fluorescence imaging applications.

Supplier: Stemcell Technologies

Fluorescent dyes used to label and track lysosomes in live cells - 500 tests.

Supplier: ADVANSTA INC MS

Make your own fluorescent antibody in one easy step.

Supplier: Anaspec

HiLyte™ Fluor 750 acid, SE is the longest-wavelength amine-reactive HiLyte™ Fluor dye currently available. Its fluorescence emission maximum at 778 nm is well separated from commonly used far-red fluorophores such as HiLyte™ Fluor 647, HiLyte™ Fluor 680 or allophycocyanin (APC), facilitating multicolor analysis.

Supplier: Anaspec

AMCA-X, SE is one of the most popular blue fluorescent tagging molecules. It is widely used to label antibodies, proteins and small drug molecules. AMCA-X succinimidyl ester contains a seven-atom aminohexanoyl spacer between the fluorophore and the reactive group.

Supplier: Surmodics

StabilZyme® HRP, StabilZyme® Protein-Free and StabilZyme® NOBLE stabilizers provide the assay developer a complete set of tools to stabilize different HRP conjugates for long term, in-solution stability.

Supplier: Anaspec

TAMRA is one of the most popular fluorophores used in various bioconjugations. 5-TAMRA is the purified single isomer of 5(6)-TAMRA. It is preferred for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between 5-TAMRA and 6-TAMRA might affect some biological properties of the underlying conjugates.

Supplier: Anaspec

Biotin, SE is amino-reactive biotinylating reagent for peptides and proteins; it has a better avidin-binding affinity than biotin. Biotin, SE (d-Biotin-N-hydroxysuccinimide ester) contains a five-carbon spacer arm that reduces steric hindrance associated with binding four biotinylated molecules per one avidin and results in enhanced detection sensitivity.

Supplier: Invitrogen

DyLight™ Dyes have absorption spectra ranging from 350 nm to 770 nm and match the output wavelengths of common fluorescence instrumentation

Supplier: Invitrogen

Thermo Scientific Pierce™ Azide-Labeled sugars provide a highly specific approach for studying glycoproteins through in vivo metabolic labeling and chemoselective ligation.

Supplier: Invitrogen

Thermo Scientific Pierce™ PEGn-SPDP is a multifunctional crosslinker for protein conjugation via amine-to-amine or amine-to-sulfhydryl crosslinks that contain a n-unit polyethylene glycol (PEG) group and a reducible (cleavable) disulfide bond.