Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
ELISA Wash Buffer (30X), Thermo Scientific
Supplier: Invitrogen
ELISA Wash Buffer is a Tris-based wash buffer for use in cytokine and other sandwich ELISA procedures. The buffer is the same formulation that is supplied with most Thermo Scientific Pierce ELISA Kits for cytokines and chemokines. The 30X concentrated stock solution is easily diluted 1:30 (vol:vol) in ultrapure water to yield sufficient wash buffer for a typical 96-well microplate ELISA protocol. Supplement an existing ELISA Kit or use as a convenient general purpose wash buffer for customized ELISA methods.
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PBS Endotoxin-free (sterile), AdipoGen Life Sciences
Supplier: Adipogen
Endotoxin free buffer used for the dilution of TLRpure LPS and solubilisation of endotoxin-free reagents (e.g
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Sodium Citrate/ Hydrochloric Acid, Buffer, Rigaku
Supplier: Rigaku Reagents
Sodium Citrate/ Hydrochloric Acid, Buffer, Rigaku
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Phosphate Buffered Saline Tablets (PBS), Accurate Chemical and Scientific
Supplier: Accurate Chemical & Scientific
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Bicine/ Sodium Hydroxide, buffer, 1M, Rigaku
Supplier: Rigaku Reagents
Bicine/ Sodium Hydroxide, buffer, 1M, Rigaku
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Cell Staining Buffer, Southern Biotechnology
Supplier: Southern Biotechnology
Cell Staining Buffer maximizes fluorescent signal intensity generated by pH sensitive fluorochromes.
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Pierce T-PER™, Tissue Protein Extraction Reagent, Thermo Scientific
Supplier: Invitrogen
Tissue Protein Extraction Reagent enables mild extraction of total protein from tissue samples.
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Poly HRP Dilution Buffer, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Pierce Poly-HRP Dilution Buffer is optimized to ensure optimal performance with Poly-HRP Streptavidin.
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Pierce™ DNA Extraction Buffer, Phenol:Chloroform and Tris Buffer, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Pierce Phenol-Chloroform is phenol supplied premixed with chloroform:isoamyl alcohol (25:24:1).
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10X Citrate Buffers, Novus Biologicals
Supplier: Novus Biologicals
10X Citrate Buffer.
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Phosphate Buffered Saline/Casein Block & Diluent-5X, BioFX®, Surmodics IVD, Inc.
Supplier: Surmodics
BioFX® Phosphate Buffered Saline/Casein Block and Diluent-5X Concentrate minimizes non-specific interactions of the secondary reagents with each other and the primary solid phase binding sites. The high blocking efficiency of casein is due to its content of small molecular weight proteins. The solution is ready for dilution to specific application requirements.
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AdvanWash™ washing solution, Advansta
Supplier: ADVANSTA INC MS
Optimized washing solutions, for Western blotting with WesternBright systems.
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Tris Buffered Saline/Casein Block & Diluent -10X, BioFX®, Surmodics IVD, Inc.
Supplier: Surmodics
BioFX® Tris Buffered Saline/Casein Block and Diluent-10X Concentrate minimizes non-specific interactions of the secondary reactants with each other and with the primary solid phase binding sites. The high blocking efficiency of casein is due to its content of small molecular weight proteins.
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AdvanBlock™-PF Blocking Solution, Advansta
Supplier: ADVANSTA INC MS
Non-protein blocking solution for fluorescent and chemiluminescent Western blotting
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Saline Solution, Sterile, 0.9%, Moltox
Supplier: MOLTOX Molecular Toxicology
Cell culture buffer, 0.9% saline solution
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Bis-tris propane/ Hydrochloric acid, buffer, 1M, Rigaku
Supplier: Rigaku Reagents
Bis-tris propane/ Hydrochloric acid, buffer, 1M, Rigaku
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