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Buffers

Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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Buffer, EDTA Buffer Solution pH 8,0 (0,5 mol/L), ROCKLAND

Supplier: Rockland Immunochemical

EDTA is widely used for scavenging metal ion in biochemistry and molecular biology

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Competent Cell Buffer, Rockland Immunochemicals

Supplier: Rockland Immunochemical

This product is ready-to-use as a working 1X solution and requires no further dilution

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RIPA Lysis Buffer, Rockland Immunochemicals

Supplier: Rockland Immunochemical

RIPA (Radio-Immunoprecipitation Assay) Lysis Buffer enables rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells.

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10X Tris-Acetate-EDTA (TAE)

Supplier: Rockland Immunochemical

10X Tris-Acetate-EDTA (TAE) Buffer

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Cell Lysis Buffer, Rockland Immunochemicals

Supplier: Rockland Immunochemical

Cell Lysis Buffer is used to lyse cells under non-denaturing conditions.

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Normal Saline

Supplier: Rockland Immunochemical

This solution is commonly referred to as physiological saline or isotonic saline.

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Running Buffers and Reagents, Protein Electrophoresis, Bio-Rad

Supplier: Bio-Rad

Bio-Rad offers a range of running buffers and reagents for many types of gels including SDS PAGE, nondenaturing, isoelectric focusing (IEF), MOPS and MES for Bis-Tris gels, and Tris-Tricine

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Imidazole/ Hydrochloric acid, buffer, 1M, Rigaku

Imidazole/ Hydrochloric acid, buffer, 1M, Rigaku

Supplier: Rigaku Reagents

Imidazole/ Hydrochloric acid, buffer, 1M, Rigaku

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Gyrolab™ Wash Buffer and Rexxip Buffers, Gyros Protein Technologies

Supplier: GYROS US INC. MS

Gyrolab™ wash buffer (pH 11) is a biodegradable high pH washing buffer that is highly efficient when used in combination with Rexxip buffers.

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Sodium Lactate/ Hydrochloric Acid, Buffer, Rigaku

Sodium Lactate/ Hydrochloric Acid, Buffer, Rigaku

Supplier: Rigaku Reagents

Sodium Lactate/ Hydrochloric Acid, Buffer, Rigaku

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StabilCoat® 5x BSA ELISA Stabilizer/Blocker, Surmodics

Supplier: Surmodics

StabilCoat® Immunoassay Stabilizer 5X concentrate, effectively preserves the conformation and activity of dried proteins while simultaneously blocking the surface to reduce non-specific binding. The product can be used to stabilize antibodies, antigens or enzymes on an assortment of immunoassay components including polystyrene plates, tubes, glass, membranes, and filter paper.

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Premixed Transfer Buffers, Western Blotting, Bio-Rad

Supplier: Bio-Rad

High-quality, ready-to-run transfer buffers in 1 L bottles and economical 5 L cubes.

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EndoTrap® HD 1/1, Biovendor

Supplier: BioVendor

EndoTrap® HD removes endotoxins (lipopolysaccharides/LPS) from aqueous solutions of a wide range of samples such as proteins, antibodies, vaccines, nucleic acids, buffers and cell extracts with excellent sample recovery rate.

The EndoTrap® HD resin is especially designed to meet the needs of pharma and biotech industry for the efficient removal of endotoxins in biomanufacturing processes as well as any application that requires highest endotoxin removal rates.

EndoTrap® HD fulfils all requirements for industrial applications, and it can easily be integrated in/at early or late-stage purification processes. Moreover, the High-Definition Matrix is also available for small-scale purification as pre-filled columns.

Endotoxin removal with EndoTrap® HD works effectively in the pH range of 4-9 and NaCl concentrations of 50-600 mM. Buffers such as HEPES, TRIS, MOPS, MES, and PIPES are recommended. Citrate buffers and chelators of divalent cations (like EDTA) have to be avoided

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Hank's Balanced Salt Solution (HBSS), Modified (Without Ca⁺⁺ and Mg⁺⁺)

Hank's Balanced Salt Solution (HBSS), Modified (Without Ca⁺⁺ and Mg⁺⁺)

Supplier: Stemcell Technologies

Hank's Balanced Salt Solution (HBSS) can be used for diluting, washing, irrigating or as a transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media, and provides cells with a source of water and essential inorganic ions and a carbohydrate as an energy source.

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Poly-HRP Dilution Buffer, Thermo Scientific

Supplier: Invitrogen

Thermo Scientific Pierce Poly-HRP Dilution Buffer is optimized to ensure optimal performance with Poly-HRP Streptavidin.

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Pierce Y-PER™, Yeast Protein Extraction Reagent, Thermo Scientific

Pierce Y-PER™, Yeast Protein Extraction Reagent, Thermo Scientific

Supplier: Invitrogen

Y-PER™ reagent uses a mild detergent lysis procedure to efficiently release functionally active solubilized proteins.

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