Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
J.T.Baker® Cell Lysis Solution, Ultrapure Bioreagent
Supplier: AVANTOR PERFORMANCE MATERIALS US
J.T.Baker® Cell Lysis Solution. Optimized multi-component, ready-to-use solution for extraction and recovery of AAV particles from HEK293 or SF9 cells. The product offers quantitative recovery through efficient cell lysis and protection of vector particles against shear stress. The solution is formulated with non-ionic detergents which are biodegradable and octyl phenol ethoxylate free.
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VWR® TBS / TWEEN® 20 Buffer
Supplier: VWR
VWR® offers a comprehensive line of buffers and detergents ideal for use in applications such as Western blotting and ELISA.
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VWR® TRIS Glycine Buffer, 10X Liquid Concentrate
Supplier: VWR
A 1X working solution contains 0.025M Tris base and 0.192 M Glycine.
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Phosphate Buffered Saline
Supplier: Ward's Science
Cell culture buffer, Phosphate Buffered Saline
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VWR® SDS-Page Gel Buffers
Supplier: VWR
The 4X concentrated buffer (0.5M Tris pH 6.8 and 0.4% SDS) for preparation of stacking gels for Laemmli SDS-Page
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VWR® TRIS-Borate-EDTA Buffer, 5X Concentrate, Ultra Pure Grade
Supplier: VWR
TBE is optimized for DNA electrophoresis techniques using both acrylamide and agarose
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VWR® Phosphate Buffered Saline (PBS) 20X, Ultra Pure Grade
Supplier: VWR
1X Solution Composition:135 mM NaCl, 2.7 mM KCl,11 mM Phosphate Buffer.Does not contain magnesium or calcium.
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VWR® Rapid Transfer Buffer, 10X
Supplier: VWR
Rapid Transfer Buffer is a simple, single component system for quick and efficient transfer of proteins from SDS-PAGE gels to membranes for Western blotting applications
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Phosphate Buffered Saline (PBS),Powdered, Ultra Pure Grade
Supplier: VWR
1X Solution Composition:137 mM NaCl,2.7 mM KCl,9.8 mM Phosphate buffer
May be sterilized by filtration.
Does not contain magnesium or calcium.
pH at 25°C (1X PBS): 7.3 – 7.5
Conductivity at 25°C (1X PBS): 13,500-17,000 umhos
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VWR® TRIS Buffer, 0.1 M Solution, pH 7.4
Supplier: VWR
Cell culture buffer, TRIS BUFFER, 0.1M SOLUTION, pH 7.4
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VWR® MOPS Buffer Solution 10X, Biotechnology Grade
Supplier: VWR
Optimized for RNA Agarose Electrophoresis
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J.T.Baker® Cell Lysis Solution, Biotech Reagent
Supplier: AVANTOR PERFORMANCE MATERIALS US
J.T.Baker® Cell Lysis Solution Biotech Reagent is a cGMP pre-formulated and ready to use detergent optimized for the extraction and recovery of AAV particles from producer cell lines (HEK293 parental and suspension lines, SF9). J.T.Baker® Cell Lysis Solution is a proprietary blend of nonionic surfactants, free of octyl phenol ethoxylate, which demonstrates comparable performance industry standard detergents while addressing environmental concerns associated with REACH and downstream clearance concerns. Designed for Gene Therapy workflows, this formulation is easy to use and handle as it is ready-to-use, has low viscosity, does not require cold temperature shipping or storage, and is available in sizes to support processes from development to large scale commercial batches. Detail description provided in technical literature.
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J.T.Baker®, pH Buffer Solutions
Supplier: AVANTOR PERFORMANCE MATERIALS US
High quality buffer solutions ensure accurate pH measurements time after time.
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VWR® Blocking Buffers
Supplier: VWR
Most routinely used blocking buffers in a variety of formats for convenience.
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VWR® EDTA 0.5M, Biotechnology Grade
Supplier: VWR
Cloning Buffer, EDTA, 0.5M Solution, pH 8.0
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