You searched for: Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Pierce™ SuperBlock™ Dry Blend Blocking Buffer, Thermo Scientific
Supplier: Invitrogen
SuperBlock (TBS) Blocking Buffer is an optimised TBS solution containing a protein that provides incredibly fast and effective blocking for ELISA, IHC and Western Blot Analysis.
Expand 1 Items
Restore™ Western Blot Stripping Buffer, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Restore™ Western Blot Stripping Buffer safely and effectively removes primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.
Expand 3 Items
Buffer Solution, Butterfields Phosphate Buffer, pH 7.2, Moltox
Supplier: MOLTOX Molecular Toxicology
Expand 1 Items
Primary Antibody Diluent, Diagnostic Biosystems
Supplier: Diagnostic Biosystems
Primary antibody diluent is specifically designed for diluting primary antibodies for immunohistochemistry. Monoclonal and polyclonal antibodies can be diluted in this buffer.
Expand 1 Items
Pierce™ Fish Serum Blocking Buffe, Thermo Scientific
Supplier: Invitrogen
This Fish Serum Blocking Buffer is Steelhead Salmon Serum in PBS that is especially useful as a blocking agent in IHC and other detection methods involving mammalian samples.
Expand 1 Items
Pierce™ Luciferase Cell Lysis Buffer (2X), Thermo Scientific
Supplier: Invitrogen
The Thermo Scientific Pierce Luciferase Cell Lysis Buffer effectively lyses adherent cells or pelleted suspension cells for analysis of promoter expression in luciferase assays.
Expand 1 Items
Effect diluents, animal-free
Supplier: ALADDIN SCIENTIFIC
Effect Diluents, Animal-free, ready-to-use (RTU)
Expand 3 Items
Potassium acetate, 1M aqua solution, pH 7.5, RNAse free, Thermo Scientific
Supplier: Thermo Scientific Chemicals
250ml CAS: 127-08-2, MDL: MFCD00012458 Liquid
Expand 1 Items
GenScript® PAGE-MASTER Protein Standard (for SDS-PAGE), GenScript
Supplier: Genscript
PAGE-MASTER Protein Standard, Consists of seven bands with molecular weight of 10 kD, 20 kD, 30 kD, 40 kD, 60 kD, 80 kD and 120 kD, All bands are highly purified, Volume: Load 5 ul/well for a mini-gel, Application: For SDS-PAGE gels, Storage: -20deg C, Size: 500ul
Expand 1 Items
Monarch® RNase A, New England Biolabs
Supplier: New England Biolabs (NEB)
Monarch® RNase A is a component of the Monarch® Genomic DNA Purification Kit (NEB #T3010), which can be used to purify genomic DNA from a variety of sample types.
Expand 1 Items
TBE (5X), TRIS + borate + EDTA, Thermo Scientific
Supplier: Thermo Scientific Chemicals
2L MDL: MFCD00236358 Liquid
Expand 2 Items
Blocking Ragents, Denhardt's Solution (50X), Thermo Scientific
Supplier: Thermo Scientific Chemicals
Denhardt's solution is used to reduce non-specific binding of detection reagents to the northern and southern blot membranes.
Expand 2 Items
FISH-Blocker™ Non-Animal Sera Protein Blocking Agent, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' FISH-Blocker™ is uses a fish protein as the primary blocking agent to eliminate cross reactivity
Expand 2 Items
5X Detector™ Block, KPL Inc.
Supplier: KPL
Detector Block is recommended as a general blocking agent for use in membrane-based detection assays
Expand 1 Items
NAP-BLOCKER™ Non-Animal Protein Blocking Agent, G-Biosciences
Supplier: G-Biosciences
A major drawback of current blocking solutions, such as BSA, casein and milk powder, is that they are derived from animal sources
Expand 3 Items
Pierce Imject™ Purification Buffer Salts, Thermo Scientific
Supplier: Invitrogen
Imject™ purification buffer salts is a dry powder for preparing 60 mL of solution for buffer-exchange of immunogens following conjugation.
Expand 1 Items
TAE Buffer, 10X Solution, Molecular Biology Grade, Ultrapure, Thermo Scientific
Supplier: Thermo Fisher Scientific
10X TAE solution consisting of 400 mM Tris and 0.01M EDTA in high purity dH₂O adjusted to pH 8.3
Expand 2 Items
Pierce™ Blocking Buffer, Protein Free, Thermo Scientific
Supplier: Invitrogen
These protein-free blocking buffers are either a PBS or a TBS formulation of a non protein compound that provides effective blocking for membrane based and plate based protein detection methods, resulting in extremely low background.
Expand 6 Items
ELISA Blocker Blocking Buffer, Thermo Scientific, Thermo Scientific
Supplier: Invitrogen
ELISA Blocker Blocking Buffer is a protein buffer that effectively blocks plate binding sites that remain in polystyrene microplate wells following initial coating steps in ELISA procedures. The ready-to-use buffer is optimized and routinely used for sandwich ELISA procedures involving cytokines, such as many of the Thermo Scientific Pierce ELISA Kits.
Expand 1 Items
Imidazole/ Hydrochloric acid, buffer, 1M, Rigaku
Supplier: Rigaku Reagents
Imidazole/ Hydrochloric acid, buffer, 1M, Rigaku
Expand 7 Items
Buffers, running buffers for Biacore Systems, Cytiva
Supplier: Cytiva
Buffers developed and verified to work in Biacore™ systems.
Expand 2 Items
Pierce Imject™ Maleimide Conjugation Buffer, Thermo Scientific
Supplier: Invitrogen
Imject™ maleimide conjugation buffer (30 mL) is sufficient for dissolving up to 60 mg of sulfhydryl-peptide for maleimide conjugation with carrier proteins.
Expand 1 Items
Buffers for Biacore Systems, Cytiva
Supplier: Cytiva
Buffers developed and verified to work in Biacore™ systems.
Expand 3 Items
Earle's Balanced Salts Solution (10X), without Magnesium Sulfate, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Earle's Balanced Salts Solution (10X), without Magnesium Sulfate, Thermo Scientific
Expand 2 Items
Gey's Balanced Salt Solution (10X), without Calcium, without Magnesium, with Phenol Red, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Gey's Balanced Salt Solution (10X), without Calcium, without Magnesium, with Phenol Red, Thermo Scientific
Expand 1 Items
EndoTrap® HD 1/1, Biovendor
Supplier: BioVendor
EndoTrap® HD removes endotoxins (lipopolysaccharides/LPS) from aqueous solutions of a wide range of samples such as proteins, antibodies, vaccines, nucleic acids, buffers and cell extracts with excellent sample recovery rate.
The EndoTrap® HD resin is especially designed to meet the needs of pharma and biotech industry for the efficient removal of endotoxins in biomanufacturing processes as well as any application that requires highest endotoxin removal rates.
EndoTrap® HD fulfils all requirements for industrial applications, and it can easily be integrated in/at early or late-stage purification processes. Moreover, the High-Definition Matrix is also available for small-scale purification as pre-filled columns.
Endotoxin removal with EndoTrap® HD works effectively in the pH range of 4-9 and NaCl concentrations of 50-600 mM. Buffers such as HEPES, TRIS, MOPS, MES, and PIPES are recommended. Citrate buffers and chelators of divalent cations (like EDTA) have to be avoided
Expand 1 Items
INDIGlo Luciferase Detection Reagent Kit
Supplier: INDIGO BIOSCIENCES INC MS
INDIGlo LDR Kit 2X- concentration Luciferase Detection Reagent and Dilution Buffer.
Expand 3 Items
pH Buffer, IDTE, 1X TE Solution, Integrated DNA Technologies
Supplier: INTEGRATED DNA TECHNOLOGIE MS
For initial resuspension and storage of DNA oligos.
Expand 1 Items
1M Tris/ Hydrochloric acid, buffer, Rigaku
Supplier: Rigaku Reagents
1M Tris/ Hydrochloric acid, buffer, Rigaku
Expand 10 Items
Pierce™ Blocker™ BLOTTO Blocking Buffer, Thermo Scientific
Supplier: Invitrogen
Blocker™ BLOTTO is a ready-to-use 5% solution of non fat powdered milk in Tris-buffered saline for Western blot, ELISA, IHC and other Detection methods.