You searched for: Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Sodium acetate, 3 M aq. soln., pH 7.0, autoclaved, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Tris(hydroxymethyl)aminomethane hydrochloride, 1M soln., pH 7,4, RNase free, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Nuclear and Cytoplasmic Extraction Kit, G-Biosciences
Supplier: G-Biosciences
For the enrichment of cytoplasmic and nuclear fractions from cultured cells and tissues
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BLOT-QuickBlocker™ Modified Milk Protein Blocking Agent, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' BLOT-QuickBlocker™ is a novel modified milk protein that is highly soluble and does not inhibit peroxidase detection
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Pierce™ DNA Extraction Buffer, Phenol:Chloroform and Tris Buffer, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Pierce Phenol-Chloroform is phenol supplied premixed with chloroform:isoamyl alcohol (25:24:1).
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Dulbecco's Phosphate Buffered Saline with 2% Fetal Bovine Serum
Supplier: STEMCELL Technologies
Dulbecco's Phosphate Buffered Saline with 2% Fetal Bovine Serum is recommended for use as a buffer to dilute or wash nucleated cells.
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Blue/Orange Loading Dye, 6X, 3ml, Promega
Supplier: Promega Corporation
Convenient marker dye used for loading DNA samples into gel electrophoresis wells and tracking migration during electrophoresis.
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Potassium acetate, 1M aq. soln., pH 7.5, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Cell Lysis Solution (CLA), Promega®
Supplier: Promega Corporation
Cell Lysis Solution is a component of the Wizard MagneSil, Wizard Plus and Wizard SV 96 and SV 9600 Plasmid DNA Purification Systems and the PureYield Plasmid Midiprep System.
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Wizard SV 96 Cell Lysis Solution, Promega®
Supplier: Promega Corporation
Cell Lysis Solution is a component of the Wizard MagneSil, Wizard Plus and Wizard SV 96 and SV 9600 Plasmid DNA Purification Systems and the PureYield Plasmid Midiprep System.
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Effect diluents, animal-free
Supplier: ALADDIN SCIENTIFIC
Effect Diluents, Animal-free, ready-to-use (RTU)
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Potassium acetate, 1M aqua solution, pH 7.5, RNAse free, Thermo Scientific
Supplier: Thermo Scientific Chemicals
250ml CAS: 127-08-2, MDL: MFCD00012458 Liquid
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Pierce™ Binding and Elution Buffers for Antibody Purification, Thermo Scientific
Supplier: Invitrogen
Buffers have been optimized to provide the highest efficiency of IgG binding and elution for most species.
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Aurion R-Gent Silver Enhancement, Electron Microscopy Sciences
Supplier: Electron Microscopy Sciences
AURION R-Gent SE-EM are high efficiency silver enhancement reagents for electron microscopy. These intensify the gold particles by homogeneous deposition of metallic silver on the particle surface. The resulting electron dense signal is easily detected and is compatible with heavy metal counterstaining.
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10X Tris-EDTA buffer pH 9.0, Novus Biologicals
Supplier: Novus Biologicals
10X Tris-EDTA buffer pH 9.0.
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GenScript® PAGE-MASTER Protein Standard (for SDS-PAGE), GenScript
Supplier: Genscript
PAGE-MASTER Protein Standard, Consists of seven bands with molecular weight of 10 kD, 20 kD, 30 kD, 40 kD, 60 kD, 80 kD and 120 kD, All bands are highly purified, Volume: Load 5 ul/well for a mini-gel, Application: For SDS-PAGE gels, Storage: -20deg C, Size: 500ul
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Monarch® RNase A, New England Biolabs
Supplier: New England Biolabs (NEB)
Monarch® RNase A is a component of the Monarch® Genomic DNA Purification Kit (NEB #T3010), which can be used to purify genomic DNA from a variety of sample types.
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TAE (50X), TRIS + acetate + EDTA, Thermo Scientific
Supplier: Thermo Scientific Chemicals
4L MDL: MFCD00236357 Liquid
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TBE (10X), TRIS + borate + EDTA, Thermo Scientific
Supplier: Thermo Scientific Chemicals
4L MDL: MFCD00236358 Liquid
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Background Blocker, Diagnostic Biosystems
Supplier: Diagnostic Biosystems
To reduce nonspecific staining during IHC.
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TBE (5X), TRIS + borate + EDTA, Thermo Scientific
Supplier: Thermo Scientific Chemicals
2L MDL: MFCD00236358 Liquid
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Prehybridization Solutions in SSC, Thermo Scientific
Supplier: Thermo Scientific Chemicals
For the hybridization of probes to nucleic acids in Nothern and Southern blotting.
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Hybridization Solution I, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' Hybridization Solution I contains: 0.9 M NaCl and 90 mM Trisodium citrate, with 5% Tween® 20.
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Blocking Ragents, Denhardt's Solution (50X), Thermo Scientific
Supplier: Thermo Scientific Chemicals
Denhardt's solution is used to reduce non-specific binding of detection reagents to the northern and southern blot membranes.
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Blocking Reagents, Denhardt's Solution (100X), Thermo Scientific
Supplier: Thermo Scientific Chemicals
Denhardt's solution is used as a blocking reagent for northern or southern blots to prevent probe binding on open membrane space and non-target nucleic acids.
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FISH-Blocker™ Non-Animal Sera Protein Blocking Agent, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' FISH-Blocker™ is uses a fish protein as the primary blocking agent to eliminate cross reactivity
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5X Detector™ Block, KPL Inc.
Supplier: KPL
Detector Block is recommended as a general blocking agent for use in membrane-based detection assays
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NAP-BLOCKER™ Non-Animal Protein Blocking Agent, G-Biosciences
Supplier: G-Biosciences
A major drawback of current blocking solutions, such as BSA, casein and milk powder, is that they are derived from animal sources