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Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

VWR® TRIS 1.0M Solution, pH 7.5, Ultra Pure Grade

VWR® TRIS 1.0M Solution, pH 7.5, Ultra Pure Grade

Supplier: VWR

TRIS 1.0M Solution pH 7.5, VWR®

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Borate Buffered Saline (BBS), Rockland Immunochemicals

Supplier: Rockland Immunochemical

Borate buffered saline (BBS) is commonly used in coating procedures for ELISA.

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J.T.Baker® DILUT-IT™, Sodium Phosphate, Dissolution Media Concentrate

J.T.Baker® DILUT-IT™, Sodium Phosphate, Dissolution Media Concentrate

Supplier: AVANTOR PERFORMANCE MATERIALS US

DILUT-IT™ dissolution media concentrate, sodium phosphate, J.T.Baker®

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J.T.Baker® DILUT-IT™, Potassium Phosphate, Dissolution Media Concentrate

J.T.Baker® DILUT-IT™, Potassium Phosphate, Dissolution Media Concentrate

Supplier: AVANTOR PERFORMANCE MATERIALS US

DILUT-IT™ is a ready-to-use dissolution media concentrate, which is pre-measured and only need to be diluted with purified water.

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2X SDS-PAGE Sample Buffer, Rockland Immunochemicals

Supplier: Rockland Immunochemical

2X SDS-PAGE Sample Buffer

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ACK Lysing Buffer, Quality Biological

ACK Lysing Buffer, Quality Biological

Supplier: QUALITY BIOLOGICAL, INC.

Ammonium-Chloride-Potassium (ACK) Lysing Buffer is used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow.

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StickTogether DNA Ligase Buffer, New England Biolabs

StickTogether DNA Ligase Buffer, New England Biolabs

Supplier: New England Biolabs (NEB)

StickTogether Buffer is supplied with T3, T7, Hi-T4 and Salt-T4 DNA ligases.

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Buffer solution pH 5, Acetate Buffer

Supplier: Thermo Scientific Chemicals

Acetate buffer, Contains 0.7 ml glacial acetic acid and 0.6 g potassium hydroxide per litre.

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GET Buffer, Rockland Immunochemicals

Supplier: Rockland Immunochemical

GET Buffer pH 8.0, 50 mM Glucose, 10 mM EDTA, 25 mM Tris HCl, pH 8.0 buffer

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Certified pH Buffer Solution, pH 7 at 20°C, Coloured (Yellow), with Fungicide, Paragon Scientific

Certified pH Buffer Solution, pH 7 at 20°C, Coloured (Yellow), with Fungicide, Paragon Scientific

Supplier: LGC Standards

Certified pH Buffer Solution, pH 7 at 20°C, Coloured (Yellow), with Fungicide, Paragon Scientific

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TBE 10X Buffer, MP Biomedicals

Supplier: MP Biomedicals

TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis, after dilution to working concentrations.

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IPG Buffers, Cytiva

IPG Buffers, Cytiva

Supplier: Cytiva

IPG buffers are ampholyte-containing buffer concentrates specifically formulated for use with Immobiline™ DryStrip gels for isoelectric focusing, run as the first dimension of 2-D electrophoresis or as a separate application. Using Immobiline DryStrip gels together with matching IPG buffer gives high sample loading capacity, improves resolution, and increases the probability of detecting low abundance proteins.

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FACS and IF Staining Buffer with BSA

Supplier: Rockland Immunochemical

Fluorescent Activated Cell Sorting (FACS) and Immunofluorescence (IF) staining buffer with BSA

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FACS and IF Staining Buffer with FBS

Supplier: Rockland Immunochemical

Fluorescent Activated Cell Sorting (FACS) and Immunofluorescence (IF) staining buffer with FBS

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PBS Buffered Saline 1X w/o Ca & Mg, Cytiva

PBS Buffered Saline 1X w/o Ca & Mg, Cytiva

Supplier: Cytiva

Cytiva manufactures PBS utilizing USP raw materials formulated in USP Water for Injection quality water.

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BlockOut® Universal Blocking Buffer for Western Blotting, Rockland Immunochemicals

Supplier: Rockland Immunochemical

BlockOut® is an universal blocking buffer solution for Western Blotting. It is specifically designed for colorimetric, chemiluminescent, and fluorescent western blotting.

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Borate Saline Buffered (BSB), Rockland Immunochemicals

Supplier: Rockland Immunochemical

Borate Saline Buffered (BSB) is commonly used in coating procedures for ELISA.

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Blocking Buffer Sampler Kit, Rockland Immunochemicals

Supplier: Rockland Immunochemical

Blocking Buffer Sampler Kit for ELISA, immunoassays, and western blot

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Buffer solutions, ready to use

Supplier: Thermo Scientific Chemicals

pH Buffer solution

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Cesium Chloride-Aluminium Nitrate Buffer Solution acc. to Schuhknecht and Schinkel for the Determination of Sodium, Potassium and Lithium by Flame Spectrometry, MilliporeSigma

Supplier: MilliporeSigma

Accordingto Schuhknecht And Schinkel For The Determination Of Sodium, Potassium And Lithium By Flame Spectrometry

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Corning® TBE Buffer, Liquid, 10X, Corning

Corning® TBE Buffer, Liquid, 10X, Corning

Supplier: Corning

TBE (Tris-Borate-EDTA) has applications in DNA and RNA agarose gel electrophoresis and polyacrylamide gel electrophoresis

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pH Gradient Buffers

Supplier: Thermo Scientific

Deliver robust, reproducible gradients with the Thermo Scientific pH buffer platform, which is applicable to a wide range of MAbs and ready to use with existing LC columns and systems, without the need to formulate mobile phases.

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RIPA Lysis Buffer, Rockland Immunochemicals

Supplier: Rockland Immunochemical

RIPA (Radio-Immunoprecipitation Assay) Lysis Buffer enables rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells.

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Corning® HEPES buffer solution (1 mol/L)

Corning® HEPES buffer solution (1 mol/L)

Supplier: Corning

HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulphonic acid) provides an alternative to the use of bicarbonate for buffering because it does not require elevated levels of CO2

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Ab Buffer Kit, Cytiva

Ab Buffer Kit, Cytiva

Supplier: Cytiva

Ab Buffer Kit contains carefully prepared buffer concentrates for binding, washing, and elution of IgG according to recommended protocols.

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Cell Lysis Buffer, Rockland Immunochemicals

Supplier: Rockland Immunochemical

Cell Lysis Buffer is used to lyse cells under non-denaturing conditions.

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Tris-Borate-EDTA (TBE) Buffer, MP Biomedicals

Supplier: MP Biomedicals

TBE buffer is a solution of 1M Tris, 0.9M boric acid and 0.01M EDTA disodium salt, prepared from MP's Ultra Pure products.

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VWR® HEPES, 1M, pH 7.3, Biotechnology Grade

VWR® HEPES, 1M, pH 7.3, Biotechnology Grade

Supplier: VWR

HEPES, 1M, pH 7.3, VWR®

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