Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
RBC lysis buffer for mouse, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Sodium Phosphate, 0.2 M Buffer Solution, pH 7.4, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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RIPA buffer-2, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Viscous liquid
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Sodium Cacodylate, 0.1 M Buffer Solution, pH 6.8
Supplier: Thermo Scientific Chemicals
Liquid
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IEF Cathode buffer (10X) with lysine and arginine, Thermo Scientific
Supplier: Thermo Scientific Chemicals
For isoelectric focusing
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Imidazole, 0.5 M Buffer Solution, pH 6.5, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Triton® X-100 lysis buffer, pH 7.4, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Blocking Reagents, BLOTTO and Tween 20 in PBS, with 0.02% Sodium Azide, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Blocking reagent for immunohistochemistry experiments.
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BSA Blocking Buffer, 5% in TBS, with 0.02% Sodium Azide, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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AMPSO, 0.2 M Buffer Solutions, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Phosphate, 0.5 M Buffer Solution, pH 9.0, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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BIS-TRIS Propane, 0.2 M Buffer Solution, pH 6.5, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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TE Buffer, (20X), pH 8.0, Autoclaved, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Blocking Reagents, Denhardt's Solution (100X), Thermo Scientific
Supplier: Thermo Scientific Chemicals
Denhardt's solution is used as a blocking reagent for northern or southern blots to prevent probe binding on open membrane space and non-target nucleic acids.
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TBE (10X), TRIS + borate + EDTA, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Running Buffer
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Sodium Bicarbonate, 1 M Buffer Solution, pH 8.0, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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