Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Pierce™ Blocking Buffer, Protein Free, Thermo Scientific
Supplier: Thermo Scientific
These protein-free blocking buffers are either a PBS or a TBS formulation of a non protein compound that provides effective blocking for membrane based and plate based protein detection methods, resulting in extremely low background.
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Antigen Retrieval Buffer (100X Citrate Buffer), Abcam
Supplier: Abcam
Antigen retrieval 100X citrate buffer (pH 5.1 - 5.5) is a special solution that enables rehydration and antigen retrieval to be performed in formalin-fixed, paraffin-embedded tissue sections with one step. - Cited in over 25 publications - Contains citrate buffer, 1.5% 2-butoxyethanol, 1% hydrochloric acid - Optimized for citrate buffer-treated antibodies - Contains detergent for paraffin emulsification
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Permeabilization Buffer (Flow Cytometry), Abcam
Supplier: Abcam
Permeabilization Buffer (ab314681) is a ready to use permeabilization buffer for intracellular immunofluorescence staining for microscopy or flow cytometry.
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Sodium acetate, 3 M aq. soln., pH 4.5, autoclaved, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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BIS-TRIS Propane, 0.2 M Buffer Solution, pH 6.5, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Laemmli SDS sample buffer with pyronin Y, reducing (4X), Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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MOPS, 1.0 M Buffer Solutions, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Imidazole, 0.2M Buffer Soln., pH 7.0, Low Endotoxin, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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BSA Blocking Buffer, 3% in TBS, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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TRIS-glycine-SDS running buffer (10X), pH 8.3, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Running Buffer
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Imidazole, 0.5 M Buffer Solution, pH 7.0, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Sodium phosphate, 0.5 M buffer soln., pH 7.6, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Sodium pyrophosphate, 200 mM buffer solution., Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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TAE (10X), TRIS + acetate + EDTA, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Running Buffer
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Phosphate-Buffered Saline (DPBS, 10X), Dulbecco's formula, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid