Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
TBE urea sample buffer (2X), Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Glycine, 0.2M Buffer Soln., pH 3.0, Low Endotoxin, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Phosphate-buffered saline (PBS, 10X), with Triton® X-100, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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BSA Blocking Buffer, 3% in PBS, with 0.02% sodium azide, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Gey's Balanced Salt Solution, with Calcium, with Magnesium, without Phenol, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Cell culture buffer, Gey's Balanced Salt Solution, with calcium, with magnesium, without phenol
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Denaturing lysis buffer, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Sodium pyrophosphate, 200 mM buffer solution., Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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PBS, 10X Solution, pH 7.4, Molecular Biology Grade, Ultrapure, Thermo Scientific
Supplier: Thermo Scientific Chemicals
10X PBS (Phosphate Buffered Saline) solution consisting of 80.6mM sodium phosphate, 19.4mM potassium phosphate, 27mM KCl and 1.37M NaCl in high purity dH2O, pH 7.4
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BES, 0.5 M Buffer Solutions, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Puck's F Balanced Salt Solution (1X), without Calcium, without Magnesium, with Phenol Red, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Cell culture buffer
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SSC (20X), RNAse Free, Thermo Scientific
Supplier: Thermo Scientific Chemicals
SSC solutions are commonly used in nucleic acid hybridization techniques at concentrations ranging from 0.1X to 20X depending on the application.
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TAE (10X), TRIS + acetate + EDTA, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Running Buffer
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Buffer, Tris(hydroxymethyl)aminomethane Hydrochloride, 1M Solution, pH 8,0, RNase-free, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Imidazole, 0.5 M Buffer Solution, pH 7.0, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Laemmli SDS sample buffer with pyronin Y, reducing (4X), Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid