Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
MOPS-EDTA-sodium acetate buffer (MESA), Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Transformation buffer-2, pH 7.5, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Phosphate, 0.5 M Buffer Solution, pH 7.2, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Sodium Phosphate, 0.2 M Buffer Solution, pH 8.0, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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GTE buffer
Supplier: Thermo Scientific Chemicals
Storage temperature: 2 to 8°C
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Maleate, 0.2 M Buffer Solution, pH 5.5, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Sodium Phosphate, 0.2 M Buffer Soln., pH 7.4, Low Endotoxin, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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TRIS-buffered saline (TBS, 10X), with 1% Tween 20, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Sodium Bicarbonate, 1 M Buffer Solution, pH 10.0, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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TRIS-buffered saline (TBS, 20X), with 2% Tween 20, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Sodium Citrate, 0.5 M Buffer Solution, pH 6.5, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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PBS 10X-Dulbecco's Phosphate Buffered Saline Solution
Supplier: FUJIFILM BIOSCIENCES INC
PBS 10X Dulbecco's Phosphate buffered saline solution can be used to provide a buffer system to maintain cell culture media in the physiological pH range, or to irrigate, transport, or dilute fluid while maintaining cell tonicity and viability.
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Pierce™ IP Lysis Buffer, Thermo Scientific
Supplier: Thermo Scientific
Pierce™ IP Lysis Buffer is optimized for cell lysate yield, purity and compatibility with immunoprecipitation (IP and Co-IP) as the downstream application for the cell lysate.
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10X Blocking Buffer, Abcam
Supplier: Abcam
10X blocking buffer (pH 7.5) for serum-free blocking of non-specific antibody binding. - Cited in over 30 publications - Suitable for ELISA, in-cell ELISA, ICC/IF, western blot (WB) - 100% casein blocking as preservative
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DIGE Buffer Kit, Cytiva
Supplier: Cytiva
DIGE buffer kit is a tool to increase the quality, reproducibility, and convenience of 2-D DIGE using the Ettan™ DIGE system. The DIGE buffer kit is a specially designed two-buffer system for running up to twelve DIGE gels simultaneously.
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1X PBS (Phosphate Buffered Saline), pH 7.4
Supplier: GROWCELLS
Balanced salt solution used in many biomolecular applications