You searched for: 5,5'-Dithiobis(2-nitrobenzoic acid) (Ellmans reagent, DTNB)

Supplier: Thermo Scientific Chemicals

5,5'-Dithiobis(2-nitrobenzoic acid) (Ellmans reagent, DTNB) 99%

Supplier: MilliporeSigma

5,5''-Dithio-bis-(2-nitrobenzoic Acid). (DTNB; Ellman'' s reagent). Pale yellow solid. PROTECT FROM LIGHT. PROTECT FROM MOISTURE. Sulfhydryl reagent used to characterize reacting SH groups. Useful in photometric determination of thiols.

Supplier: Thermo Scientific Chemicals

Reagent for determination of sufhydryl groups

Supplier: Enzo Life Sciences

Sensitive sulfhydyl reagent

Supplier: Ambeed

5,5'-Dithiobis(2-nitrobenzoic acid) (Ellmans reagent, DTNB) 98%

Supplier: G-Biosciences

Ellman's reagent is a versatile, water-soluble compound for quantifying free sulfhydryl groups in solution. It reacts with a free sulfhydryl group to yield a mixed disulfide and 2-nitro-5-thiobenzoic acid (NTB), a measurable yellow colored product at 412nm.
Ellman's reagent is very useful as a free sulfhydryl assay reagent due to its high specificity for -SH groups at neutral pH, high molar extinction coefficient and short reaction time.

Supplier: BeanTown Chemical

CAS: 69-78-3; EC No: 200-714-4; MDL No: MFCD00007140; RTECS: DG9650000 Powder; Molecular Formula: C14H8N2O8S2; MW: 396.35 Melting Point: 240-245° (decomposes)

Supplier: Matrix Scientific

5,5'-Dithiobis(2-nitrobenzoic acid) (Ellmans reagent, DTNB) ≥97%

Supplier: Adipogen

A sensitive reagent for measuring the free sulfhydryl content in proteins, peptides, and tissues. Used to characterize reactive thiol groups and photometric determination of thiols and for measuring low-molecular mass thiols such as glutathione in both pure solutions and biological samples, such as blood. It can also measure the number of thiol groups on proteins. Through reaction with aliphatic thiol groups a mixed disulfide of protein thiol and one mole of 2-nitro-5-thiobenzoate per mole of protein sulfhydryl group is being formed. DTNB has little absorbance. Reaction with -SH groups on proteins (from any solvent accessible Cys) under mild alkaline conditions (pH 7-8) produces the 2-nitro-5-thiobenzoate anion, which gives an intense yellow color with an absorption maximum at 409.5nm (Extinction coefficient: 14150 M-1*cm-1). Sensitive to various buffer ions, therefore, the extinction coefficient used to calculate the number of sulfhydryl groups must be matched to the reaction conditions. In case the thiol groups are in disulfide bonds, they must be reduced under anaerobic conditions prior to reaction with DTNB.

Supplier: Invitrogen

Thermo Scientific Pierce Ellman's Reagent (DTNB) reacts with sulfhydryl groups to yield a colored product, providing a reliable method to measure reduced cysteines and other free sulfhydryls in solution.