You searched for: Urea

Supplier: AVANTOR PERFORMANCE MATERIALS US

Urea, U.S.P.

Supplier: AVANTOR PERFORMANCE MATERIALS US

Urea, U.S.P.

Supplier: VWR International

Urea 99.0-100.5%, crystallised ACS, VWR Chemicals BDH®

Supplier: VWR

Commonly used to denature nucleic acids for electrophoresis and to study the secondary and tertiary structure of proteins. Effective for maintaining DNA in a highly denatured state during sequencing protocols, urea is also useful for accelerating the activity of Proteinase K digestions. Effectively denatures RNA. For use in nucleic electrophoresis applications.

Supplier: VWR

Commonly used to denature nucleic acids for electrophoresis and to study the secondary and tertiary structure of proteins. Effective for maintaining DNA in a highly denatured state during sequencing protocols, urea is also useful for accelerating the acti

Supplier: AVANTOR PERFORMANCE MATERIALS US

GMP manufactured.

Supplier: AVANTOR PERFORMANCE MATERIALS US

For protein solubilization and denaturation. DNase-, RNase-, protease-, and cyanate-free.

Supplier: VWR

A convenient pre-mixed solution that is easily diluted for buffer preparation under denaturing conditions.Commonly used to denature nucleic acids for electrophoresis and to study the secondary and tertiary structure of proteins. Effective for maintaining DNA in a highly denatured state during sequencing protocols, urea is also useful for accelerating the activity of Proteinase K digestions.

Supplier: AVANTOR PERFORMANCE MATERIALS US

Crystals. GMP manufactured.

Supplier: AVANTOR PERFORMANCE MATERIALS US

Lot analysis on label.

Supplier: AVANTOR PERFORMANCE MATERIALS US

Meets USP requirements. Meets BP/Ph. Eur. and JP chemical specifications. GMP manufactured product.

Supplier: AVANTOR PERFORMANCE MATERIALS US

Crystals.

Supplier: Avantor Performance Materials

Suitable for bioprocessing. BSE/TSE free. Endotoxin tested. Custom packaging and testing available. Change management and notification available. Samples available.

Supplier: MilliporeSigma

Urea for synthesis, Sigma-Aldrich®

Supplier: MilliporeSigma

Supplier: Thermo Scientific Chemicals

Urea 99% ACS

Supplier: MP Biomedicals

Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C

Supplier: MP Biomedicals

Urea is a prinicipal protein metabolite end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle and the major product for the removal of free ammonia (NH4+) in vivo.

Supplier: MP Biomedicals

Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Urea is a mild agent usually used in the solubilization and denaturation of proteins. It is also useful for renaturing proteins from samples already denatured with 6 M guanidine hydrochloride such as inclusion bodies; and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose; used as a diuretic; enhances the action of sulfonamides; an antiseptic.
Urea in solution is in equilibrium with ammonium cyanate. The form that reacts with protein amino groups is isocyanic acid. Urea in the presence of heat and protein leads to carbamylation of the proteins. Carbamylation by isocyanic acid interferes with protein characterization because isocyanic acid reacts with the amino terminus of proteins, preventing N-terminal sequencing. Isocyanic acid also reacts with side chains of lysine and arginine residues resulting in a protein that is unsuitable for many enzymatic digests. In addition, carbamylation often leads to confusing results from peptides having unexpected retention times and masses. When performing enzymatic protein digests it is important to remove urea first. Even though some enzymes will tolerate small amounts of urea, the elevated temperature used for most reactions will lead to carbamylation during the course of the digest. The urea can be removed prior to digestion by fast reversed phase chromatography, spin columns, or dialysis.
Dissolve urea in deionized water to the desired concentration.For every 10 ml of solution, add 1 g of Amberlite® IRA-910.Stir for one hour at room temperature

Supplier: Thermo Scientific Chemicals

MDL: MFCD00008022 Beilstein Registry No.: 635724 Fieser: 1,1262 2,455

Supplier: Thermo Scientific Chemicals

Used as a protein denaturant

Supplier: Thermo Scientific Chemicals

Urea 98%, pearls, Extra Pure

Supplier: Cytiva

500 g

Supplier: MP Biomedicals

Urea is a mild agent usually used in the solubilization and denaturation of proteins.

Supplier: MilliporeSigma

(Carbimide; carbonyl diamide). White solid. PROTECT FROM MOISTURE. End product of the urea cycle. A topical keratolytic agent. Soluble in H2O. RTECS YR6250000, CAS 57-13-6, M.W. 60.1. WARNING! May be carcinogenic/teratogenic.

Supplier: Thermo Scientific Chemicals

Urea 99.5% for molecular biology DNase-, RNase-, protease-free

Supplier: MP Biomedicals

Urea is a mild agent usually used in the solubilization and denaturation of proteins. It is also useful for renaturing proteins from samples already denatured with 6 M guanidine hydrochloride such as inclusion bodies; and in the extraction of the mitochondrial complex.

Supplier: MilliporeSigma

Ideal for protein unfolding/denaturation.

Supplier: Spectrum Chemical Mfg. Corp.

Urea, USP, JP is used in topical dermatological products to promote rehydration of the skin. Urea preparations are also used for non-surgical removal of damaged or dead nails. All Spectrum Chemical USP, JP products are manufactured, packaged and stored under current Good Manufacturing Practices (cGMP) per 21CFR part 211 in FDA registered and inspected facilities

Supplier: Promega Corporation

Urea is a protein denaturant. Urea is qualified for use as the denaturing component in polyacrylamide gels.