Urea
Urea 98.0-102.0% USP, Ph. Eur., BP, JP endotoxin tested
Supplier: Avantor Performance Materials
Suitable for bioprocessing. BSE/TSE free. Endotoxin tested. Custom packaging and testing available. Change management and notification available. Samples available.
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Urea, high purity
Supplier: VWR
Commonly used to denature nucleic acids for electrophoresis and to study the secondary and tertiary structure of proteins. Effective for maintaining DNA in a highly denatured state during sequencing protocols, urea is also useful for accelerating the activity of Proteinase K digestions. Effectively denatures RNA. For use in nucleic electrophoresis applications.
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Urea 99.0-100.5%, crystals, AR® ACS, Macron Fine Chemicals™
Supplier: AVANTOR PERFORMANCE MATERIALS US
Crystals.
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Urea ≥99.5%, Ultrapure Bioreagent
Supplier: AVANTOR PERFORMANCE MATERIALS US
For protein solubilization and denaturation. DNase-, RNase-, protease-, and cyanate-free.
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Urea 99.0-100.5%, crystallised ACS, VWR Chemicals BDH®
Supplier: VWR International
Urea 99.0-100.5%, crystallised ACS, VWR Chemicals BDH®
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Urea 99.0-100.5% USP
Supplier: AVANTOR PERFORMANCE MATERIALS US
Urea 99.0-100.5% USP
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Urea 8 M in aqueous solution, Proteomics Grade
Supplier: VWR
A convenient pre-mixed solution that is easily diluted for buffer preparation under denaturing conditions.Commonly used to denature nucleic acids for electrophoresis and to study the secondary and tertiary structure of proteins. Effective for maintaining DNA in a highly denatured state during sequencing protocols, urea is also useful for accelerating the activity of Proteinase K digestions.
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Urea, Proteomics Grade
Supplier: VWR
Commonly used to denature nucleic acids for electrophoresis and to study the secondary and tertiary structure of proteins. Effective for maintaining DNA in a highly denatured state during sequencing protocols, urea is also useful for accelerating the acti
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Urea 98.0-102.0% USP, Multi-Compendial
Supplier: AVANTOR PERFORMANCE MATERIALS US
Meets USP requirements. Meets BP/Ph. Eur. and JP chemical specifications. GMP manufactured product.
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Urea ≥99% USP, Macron Fine Chemicals™
Supplier: AVANTOR PERFORMANCE MATERIALS US
Crystals. GMP manufactured.
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Urea ≥99.5%, BAKER ANANYZED™ ACS
Supplier: AVANTOR PERFORMANCE MATERIALS US
Lot analysis on label.
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Urea for synthesis, Sigma-Aldrich®
Supplier: MilliporeSigma
Urea for synthesis, Sigma-Aldrich®
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Urea ≥99%, white prills, Ultrapure
Supplier: MP Biomedicals
Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Urea is a mild agent usually used in the solubilization and denaturation of proteins. It is also useful for renaturing proteins from samples already denatured with 6 M guanidine hydrochloride such as inclusion bodies; and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose; used as a diuretic; enhances the action of sulfonamides; an antiseptic.
Urea in solution is in equilibrium with ammonium cyanate. The form that reacts with protein amino groups is isocyanic acid. Urea in the presence of heat and protein leads to carbamylation of the proteins. Carbamylation by isocyanic acid interferes with protein characterization because isocyanic acid reacts with the amino terminus of proteins, preventing N-terminal sequencing. Isocyanic acid also reacts with side chains of lysine and arginine residues resulting in a protein that is unsuitable for many enzymatic digests. In addition, carbamylation often leads to confusing results from peptides having unexpected retention times and masses. When performing enzymatic protein digests it is important to remove urea first. Even though some enzymes will tolerate small amounts of urea, the elevated temperature used for most reactions will lead to carbamylation during the course of the digest. The urea can be removed prior to digestion by fast reversed phase chromatography, spin columns, or dialysis.
Dissolve urea in deionized water to the desired concentration.For every 10 ml of solution, add 1 g of Amberlite® IRA-910.Stir for one hour at room temperature
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