You searched for: 5(6)-Carboxyfluorescein N-hydroxysuccinimide ester (5/6-FAM SE)

Supplier: CHEM-IMPEX INTERNATIONAL, INC

5(6)-Carboxyfluorescein N-hydroxysuccinimide ester (5/6-FAM SE)

Supplier: Ambeed

5(6)-Carboxyfluorescein N-hydroxysuccinimide ester (5/6-FAM SE) ≥95% (mixture of isomers)

Supplier: AAT BIOQUEST INC

Carboxyfluorescein (commonly called FAM) and its amine-reactive succinimidyl esters are favored over FITC in bioconjugations.

Supplier: Biotium

5(6)-FAM SE (full name: 5-(and-6)-Carboxyfluorescein, succinimidyl ester mixed isomers) is an amine-reactive green fluorescent dye widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine. The coupling reaction is usually carried out at pH 8-9.5. The amide linkage from the coupling reaction is much more stable than the thiourea linkage formed from the coupling of an amine and an isothiocyanate.

Supplier: Invitrogen

NHS fluorescein [(5/6-carboxyfluorescein succinimidyl ester), mixed isomer] is an amine reactive derivative of fluorescein dye for antibodies and other probes in fluorescence microscopy, flow cytometry, and immunofluorescence based assays such as western blotting and ELISA.

Supplier: Anaspec Inc

Although FITC reagents have been more often used to prepare fluoresceinated bioconjugates, the low stability of FITC bioconjugates makes some researchers use amine-reactive succinimidyl esters of carboxyfluorescein (commonly called FAM) in bioconjugations. FAM reagents give carboxamides that are more resistant to hydrolysis. We have shown that FAM reagents require less stringent reaction conditions and give better conjugation yields, and the resulted conjugates have superior stability. We noted that FITC labeled peptides tend to deteriorate more quickly than the corresponding FAM conjugates.