Extraction Buffers
Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.
Sodium Dodecyl Sulfate SDS, 20%
Supplier: Rockland Immunochemical
SDS, sodium dodecyl sulfate is an anionic surfactant.
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Monarch® Buffer BZ
Supplier: New England Biolabs (NEB)
Monarch Buffer BZ is included in Monarch nucleic acid purification kits, and also offered as a standalone buffer for convenience.
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Monarch® Precipitation Enhancer, New England Biolabs
Supplier: New England Biolabs (NEB)
The Monarch® precipitation enhancer solution, supplied as a component of the Monarch® HMW DNA extraction kit for cells and blood, is a salt-based solution that facilitates the precipitation of DNA onto the Monarch® DNA capture beads during HMW DNA extraction from cells and blood.
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RIPA Buffer, Cell Biolabs
Supplier: Cell Biolabs
Cell Biolabs' RIPA Buffer is suitable for the extraction of cytoplasmic, membrane, and nuclear proteins from cells or tissues
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Pierce Y-PER™, Yeast Protein Extraction Reagent, Thermo Scientific
Supplier: Invitrogen
Y-PER™ reagent uses a mild detergent lysis procedure to efficiently release functionally active solubilized proteins.
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Pierce Subcellular Protein Fractionation Kit, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Subcellular Protein Fractionation Kit for Cultured Cells enables segregation and enrichment of proteins from five different cellular compartments.
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Rapid GST Inclusion Body Solubilization and Renaturation Kit, Cell Biolabs
Supplier: Cell Biolabs
Recombinant proteins expressed in bacteria often form inclusion bodies, especially when they are expressed at high levels
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Pierce M-PER™, Mammalian Protein Extraction Reagents, Thermo Scientific
Supplier: Invitrogen
M-PER™ Mammalian Protein Extraction Reagent is designed to provide highly efficient total protein extraction from cultured mammalian cells.
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Pierce™ RIPA Buffer, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific RIPA Buffer is a high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent for cultured mammalian cells.
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Pierce Y-PER™ Plus Dialyzable, Yeast Protein Extraction Reagent, Thermo Scientific
Supplier: Invitrogen
This extraction reagent is formulated to effectively lyse yeast and bacterial cells to extract and solubilize proteins for analysis
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Ammonium Chloride Solution
Supplier: Stemcell Technologies
Ammonium chloride solution is recommended for the lysis of red blood cells (RBCs) in preparations of human and mouse peripheral blood, spleen or bone marrow cells.
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Pierce T-PER™, Tissue Protein Extraction Reagent, Thermo Scientific
Supplier: Invitrogen
Tissue Protein Extraction Reagent enables mild extraction of total protein from tissue samples.
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Pierce™ DNA Extraction Buffer, Phenol:Chloroform and Tris Buffer, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Pierce Phenol-Chloroform is phenol supplied premixed with chloroform:isoamyl alcohol (25:24:1).
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Pierce™ Luciferase Cell Lysis Buffer (2X), Thermo Scientific
Supplier: Invitrogen
The Thermo Scientific Pierce Luciferase Cell Lysis Buffer effectively lyses adherent cells or pelleted suspension cells for analysis of promoter expression in luciferase assays.
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Pierce I-PER™, Insect Cell Protein Extraction Reagent, Thermo Scientific
Supplier: Invitrogen
I-PER™ Insect Cell Protein Extraction Reagent enables gentle and effective extraction of soluble protein from baculoviral-infected insect cells grown in suspension or monolayer culture.
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RNAdvance Viral RNA Extraction Kit, Beckman Coulter
Supplier: Beckman Coulter
Extract high-quality RNA from saliva, nasopharyngeal and oropharyngeal swab collection devices.
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