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Extraction Buffers

Extraction Buffers

Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.

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Cell Lysis Solution (CLA), Promega®

Cell Lysis Solution (CLA), Promega®

Supplier: Promega Corporation

Cell Lysis Solution is a component of the Wizard MagneSil, Wizard Plus and Wizard SV 96 and SV 9600 Plasmid DNA Purification Systems and the PureYield Plasmid Midiprep System.

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DNA Elution Buffer, Zymo Research

DNA Elution Buffer, Zymo Research

Supplier: Zymo Research

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Mammalian Lysis Buffer, Promega

Mammalian Lysis Buffer, Promega

Supplier: Promega Corporation

The HaloTag Mammalian Pull-Down Systems capture and purify intracellular protein complexes for correlative cellular localization and real-time imaging studies with the same genetic construct for further understanding of overall protein function.

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Monarch® HMW gDNA Tissue Lysis Buffer, New England Biolabs

Monarch® HMW gDNA Tissue Lysis Buffer, New England Biolabs

Supplier: New England Biolabs (NEB)

The Monarch® HMW gDNA tissue lysis buffer, supplied as a component of the Monarch HMW DNA extraction kit for tissue, is a highly effective lysis buffer for tissue and bacteria samples. Its composition ensures immediate and complete inactivation of nucleases in the samples at the start of the lysis.

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Wizard SV 96 Cell Lysis Solution, Promega®

Wizard SV 96 Cell Lysis Solution, Promega®

Supplier: Promega Corporation

Cell Lysis Solution is a component of the Wizard MagneSil, Wizard Plus and Wizard SV 96 and SV 9600 Plasmid DNA Purification Systems and the PureYield Plasmid Midiprep System.

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Monarch® gDNA Wash Buffer, New England Biolabs

Supplier: New England Biolabs (NEB)

The Monarch® gDNA Wash Buffer a component of the Monarch® Genomic DNA Purification Kit (NEB #T3010), which can be used to purify up to 30 µg genomic DNA from a variety of sample types.

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Monarch® gDNA Elution Buffer II, New England Biolabs

Monarch® gDNA Elution Buffer II, New England Biolabs

Supplier: New England Biolabs (NEB)

The Monarch® gDNA elution buffer II is supplied as a component of the Monarch HMW DNA extraction kits.

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Lysis Buffer, 1X, Rockland Immunochemicals

Supplier: Rockland Immunochemical

1X Lysis Buffer

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Protein Extraction and Lysis Buffer (PE LB™) Systems, G-Biosciences

Protein Extraction and Lysis Buffer (PE LB™) Systems, G-Biosciences

Supplier: G-Biosciences

Lysis and extraction of biologically active proteins from cellular and tissue samples is the first critical step for biochemical analysis

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Cell Lysis Solution, Promega®

Cell Lysis Solution, Promega®

Supplier: Promega Corporation

Cell Lysis Solution is a component of the Wizard MagneSil, Wizard Plus and Wizard SV 96 and SV 9600 Plasmid DNA Purification Systems and the PureYield Plasmid Midiprep System.

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ProXtract B™ Extraction Buffer, Rockland Immunochemicals

Supplier: Rockland Immunochemical

ProXtract B™ is ready-to-use as a working 1X solution and requires no further dilution.

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ReadiUse™ Viral RNA Lysis Buffer, AAT Bioquest

ReadiUse™ Viral RNA Lysis Buffer, AAT Bioquest

Supplier: AAT Bioquest

Current demands for RT-PCR testing of SARS-CoV-2 has led to a worldwide shortage of RNA extraction kits.

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Luna Cell Ready Lysis Module, New England Biolabs

Luna Cell Ready Lysis Module, New England Biolabs

Supplier: New England Biolabs (NEB)

Cell cultures are often analyzed for gene expression or treatment responses as a proxy for a living organism. Traditionally, RNA is extracted and purified from treated cells via column-based or chemical methods. The Luna Cell Ready Lysis Module is a fast, convenient, and sensitive alternative for evaluating RNA expression levels without performing traditional RNA extraction steps.

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Red Blood Cell Lysis Solution (CLB), 200ml, Promega

Red Blood Cell Lysis Solution (CLB), 200ml, Promega

Supplier: Promega Corporation

Component of the SV Total RNA Isolation and PureYield RNA Midiprep Systems.

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Fluorophos® Cheese Extraction Buffer, Advanced Instruments

Supplier: Advanced Instruments

The fluorophos cheese extraction buffer is used to extract alkaline phosphatase from cheese products.

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Passive Lysis 5X Buffer, Promega

Passive Lysis 5X Buffer, Promega

Supplier: Promega Corporation

Promotes rapid lysis of cultured mammalian cells without the need to scrape adherent cells or freeze-thaw. Lytic reagent of choice when processing cells for quantitation of firefly and Renilla luciferase activities using the Promega Dual-Luciferase® Reporter Assay (#PAE1910).

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