Extraction Buffers
Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.
SideStep II Cell Lysis Analysis Kit, Agilent Technologies
Supplier: Agilent Technologies
Progress from mammalian cells to qPCR or QRT-PCR experiments without nucleic acid purification.
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RIPA buffer-2 (2X), Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Extraction Buffer 5X PTR, Abcam
Supplier: Abcam
Extraction buffer 5X PTR is formulated for preparing cell or tissue extracts for use in immunoassays, with added phosphatase inhibitors and protease inhibitor aprotinin. - Cited in over 20 publications - Phosphatase and protease inhibitors – contains inhibitors to preserve sample integrity - Easy to use – dilute to 1X with distilled water before use, or use with extraction enhancer buffer 50X for enhanced extraction - Compatible with a variety of sample types
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MES lysis buffer with Triton® X-100, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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RBC lysis buffer for mouse, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Nuclear Extraction Kit, Abcam
Supplier: Abcam
This Nuclear Extraction Kit is formulated for the quick and simple isolation of nuclear and cytoplasmic fractions from cultured cells and tissue homogenates that can be used successfully in our transcription factor assay kits.
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RIPA buffer, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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RIPA Buffer, Abcam
Supplier: Abcam
A ready-to-use buffer useful for lysing cells and tissues for radioimmunoprecipitation assay.
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HeLa Nuclear Extract, Abcam
Supplier: Abcam
Nuclear extract is prepared from HeLa cells, which contains a variety of DNA-binding proteins, transcription factors, and other nuclear proteins.
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Extraction Enhancer Buffer 50X, Abcam
Supplier: Abcam
Abcam's Extraction Enhancer Buffer 50X is formulated for the preparation of cell or tissues extracts for use in immunoassays.
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RBC Lysis Buffer (10X), Tonbo Biosciences
Supplier: Tonbo Biosciences
RBC Lysis Buffer (10X) is a concentrated ammonium chloride-based lysing reagent
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RIPA Cell Lysis Buffer 3, (100 ml), Enzo Life Sciences
Supplier: Enzo Life Sciences
Liquid.
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RIPA buffer with EDTA, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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HEPES lysis buffer with Triton® X-100
Supplier: Thermo Scientific Chemicals
Liquid
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NP-40 lysis buffer with glycerol (2X), Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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HEPES protein extraction buffer (5X)
Supplier: Thermo Scientific Chemicals
Liquid
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