Extraction Buffers
Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.
HEPES lysis buffer with NP-40 (2X)
Supplier: Thermo Scientific Chemicals
Liquid
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GTE buffer
Supplier: Thermo Scientific Chemicals
Storage temperature: 2 to 8°C
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RIPA buffer-2, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Viscous liquid
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Pierce™ IP Lysis Buffer, Thermo Scientific
Supplier: Thermo Scientific
Pierce™ IP Lysis Buffer is optimized for cell lysate yield, purity and compatibility with immunoprecipitation (IP and Co-IP) as the downstream application for the cell lysate.
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Phusion HF® Buffer Pack, New England Biolabs
Supplier: New England Biolabs (NEB)
New England Biolabs supplies a unique 5X reaction buffer and supplemental reagents for use with Thermo Scientific® Phusion High-Fidelity DNA Polymerase and associated products, all of which are manufactured and quality controlled at New England Biolabs.
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Cell lysis buffer 4, (100 ml), Enzo Life Sciences
Supplier: Enzo Life Sciences
100 mL
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RIPA buffer with Triton® X-100 (2X), Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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RIPA buffer-2 (2X), Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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NP-40 lysis buffer (2X), Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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RIPA buffer (2X), Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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SideStep II Cell Lysis Analysis Kit, Agilent Technologies
Supplier: Agilent Technologies
Progress from mammalian cells to qPCR or QRT-PCR experiments without nucleic acid purification.
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Extraction Buffer 5X PTR, Abcam
Supplier: Abcam
Extraction buffer 5X PTR is formulated for preparing cell or tissue extracts for use in immunoassays, with added phosphatase inhibitors and protease inhibitor aprotinin. - Cited in over 20 publications - Phosphatase and protease inhibitors – contains inhibitors to preserve sample integrity - Easy to use – dilute to 1X with distilled water before use, or use with extraction enhancer buffer 50X for enhanced extraction - Compatible with a variety of sample types
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Caspase Cell Lysis Buffer, Enzo Life Sciences
Supplier: Enzo Life Sciences
Enzo Life Sciences
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Succinate, 0.2M buffer soln., pH 5.0, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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RIPA buffer with Triton® X-100 (5X), Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Phosphate buffered RIPA, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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