Extraction Buffers
Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.
VWR® Hypotonic Lysis Buffer
Supplier: VWR
Cell lysis in detergent-free Hypotonic Lysis buffer and the subsequent isolation of the crude cytoplasmic fraction can be completed in less than 30 minutes
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J.T.Baker® Cell Lysis Solution, Ultrapure Bioreagent
Supplier: AVANTOR PERFORMANCE MATERIALS US
J.T.Baker® Cell Lysis Solution. Optimized multi-component, ready-to-use solution for extraction and recovery of AAV particles from HEK293 or SF9 cells. The product offers quantitative recovery through efficient cell lysis and protection of vector particles against shear stress. The solution is formulated with non-ionic detergents which are biodegradable and octyl phenol ethoxylate free.
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J.T.Baker® Cell Lysis Solution, Biotech Reagent
Supplier: AVANTOR PERFORMANCE MATERIALS US
J.T.Baker® Cell Lysis Solution Biotech Reagent is a cGMP pre-formulated and ready to use detergent optimized for the extraction and recovery of AAV particles from producer cell lines (HEK293 parental and suspension lines, SF9). J.T.Baker® Cell Lysis Solution is a proprietary blend of nonionic surfactants, free of octyl phenol ethoxylate, which demonstrates comparable performance industry standard detergents while addressing environmental concerns associated with REACH and downstream clearance concerns. Designed for Gene Therapy workflows, this formulation is easy to use and handle as it is ready-to-use, has low viscosity, does not require cold temperature shipping or storage, and is available in sizes to support processes from development to large scale commercial batches. Detail description provided in technical literature.
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Denaturing lysis buffer, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Extraction Reagents 2 (5X), (10 ml), Enzo Life Sciences
Supplier: Enzo Life Sciences
Concentrated lysis buffer for preparation of cell and tissue extracts.
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NP-40 lysis buffer, low salt, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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RIPA buffer with EDTA + EGTA, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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PIPES lysis buffer with NP-40 (2X), Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Extraction Reagents 1 (5X), (10 ml), Enzo Life Sciences
Supplier: Enzo Life Sciences
Concentrated lysis buffer for preparation of cell and tissue extracts.
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Cell Lysis Buffer, Abcam
Supplier: Abcam
ab152163 is a 10X Cell Lysis Buffer used to lyse cells under non-denaturing conditions.
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RBC lysis buffer for human, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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ACK Lysing Buffer, Quality Biological
Supplier: QUALITY BIOLOGICAL, INC.
Ammonium-Chloride-Potassium (ACK) Lysing Buffer is used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow.
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Nuclear (Nucleic Acid-Free) Extraction Kit, Abcam
Supplier: Abcam
Nuclear (Nucleic Acid-Free) Extraction Kit (ab113477) provides a simple and selective method for extracting nuclear proteins (nucleic-acid free) in just 1 hour for a variety of applications such as western blotting, protein-DNA binding assays, or any other procedures requiring optimized nucleic acid-free nuclear proteins.
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Nuclear Extraction Kit, Abcam
Supplier: Abcam
Nuclear extraction kit provides a simple and selective method for nuclear protein extraction and fractionation from cells or tissue samples in just 1 hour. This kit includes all necessary reagents to obtain high yields of nuclear proteins suitable for various downstream applications. - Cited in over 255 publications - High retention of enzyme activity – contains 80% less salt than conventional kits and no SDS, allowing better enzyme activity retention - Suitable for western blotting, protein-DNA binding assays, nuclear enzyme assays, and more - Fast, simple procedure that ensures optimized nuclear protein isolation
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