Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.
VWR® Hypotonic Lysis Buffer
Supplier: VWR
Cell lysis in detergent-free Hypotonic Lysis buffer and the subsequent isolation of the crude cytoplasmic fraction can be completed in less than 30 minutes
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J.T.Baker® Cell Lysis Solution, Biotech Reagent
Supplier: AVANTOR PERFORMANCE MATERIALS US
J.T.Baker® Cell Lysis Solution Biotech Reagent is a cGMP pre-formulated and ready to use detergent optimized for the extraction and recovery of AAV particles from producer cell lines (HEK293 parental and suspension lines, SF9). J.T.Baker® Cell Lysis Solution is a proprietary blend of nonionic surfactants, free of octyl phenol ethoxylate, which demonstrates comparable performance industry standard detergents while addressing environmental concerns associated with REACH and downstream clearance concerns. Designed for Gene Therapy workflows, this formulation is easy to use and handle as it is ready-to-use, has low viscosity, does not require cold temperature shipping or storage, and is available in sizes to support processes from development to large scale commercial batches. Detail description provided in technical literature.
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ProXtract B™ Extraction Buffer, Rockland Immunochemicals
Supplier: Rockland Immunochemical
ProXtract B™ is ready-to-use as a working 1X solution and requires no further dilution.
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RIPA Lysis and Extraction Buffer, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' RIPA Lysis and Extraction Buffer is a complete lysis buffer for the release of cytoplasmic, membrane and nuclear proteins from adherent and suspension cultured mammalian cells
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Fluorophos® Cheese Extraction Buffer, Advanced Instruments
Supplier: Advanced Instruments
The fluorophos cheese extraction buffer is used to extract alkaline phosphatase from cheese products.
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FOCUS™ Extraction Buffers, G-Biosciences
Supplier: G-Biosciences
One of the most important considerations before running 2D gel electrophoresis is the choice of protein solubilization buffers
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Extraction Reagents 1 (5X), (10 ml), Enzo Life Sciences
Supplier: Enzo Life Sciences
Concentrated lysis buffer for preparation of cell and tissue extracts.
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Extraction Reagents 2 (5X), (10 ml), Enzo Life Sciences
Supplier: Enzo Life Sciences
Concentrated lysis buffer for preparation of cell and tissue extracts.
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Buffers for E.Z.N.A.® Gel Extraction Systems, Omega Bio-tek®
Supplier: Omega Bio-Tek
Binding Buffer (XP2), Omega Bio-Tek
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Protein Extraction and Lysis Buffer (PE LB™) Systems, G-Biosciences
Supplier: G-Biosciences
Lysis and extraction of biologically active proteins from cellular and tissue samples is the first critical step for biochemical analysis
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ACK Lysing Buffer, Quality Biological
Supplier: QUALITY BIOLOGICAL, INC.
Ammonium-Chloride-Potassium (ACK) Lysing Buffer is used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow.
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RIPA Lysis Buffer, Rockland
Supplier: Rockland Immunochemical
RIPA (Radio-Immunoprecipitation Assay) Lysis Buffer enables rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells
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SideStep II Cell Lysis Analysis Kit, Agilent Technologies
Supplier: Agilent Technologies
Progress from mammalian cells to qPCR or QRT-PCR experiments without nucleic acid purification.
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Phusion HF® Buffer Pack, New England Biolabs
Supplier: New England Biolabs (NEB)
New England Biolabs supplies a unique 5X reaction buffer and supplemental reagents for use with Thermo Scientific® Phusion High-Fidelity DNA Polymerase and associated products, all of which are manufactured and quality controlled at New England Biolabs.
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Sodium Dodecyl Sulfate SDS, 20%
Supplier: Rockland Immunochemical
SDS, sodium dodecyl sulfate is an anionic surfactant.
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BipPulverizer System II, MP Biomedicals
Supplier: MP Biomedicals
BioPulverizer™ System II contains 10x2 mL tubes of each of our specialized Lysing Matrices.
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Monarch® gDNA Elution Buffer II, New England Biolabs
Supplier: New England Biolabs (NEB)
The Monarch® gDNA elution buffer II is supplied as a component of the Monarch HMW DNA extraction kits.
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Lysis Buffer, 1X, Rockland Immunochemicals
Supplier: Rockland Immunochemical
1X Lysis Buffer
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RBC Lysis Buffer (10X), Tonbo Biosciences
Supplier: Tonbo Biosciences
RBC Lysis Buffer (10X) is a concentrated ammonium chloride-based lysing reagent
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RIPA Cell Lysis Buffer 3, (100 ml), Enzo Life Sciences
Supplier: Enzo Life Sciences
Liquid.
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Cell Lysis Buffer, Rockland Immunochemicals
Supplier: Rockland Immunochemical
Cell Lysis Buffer is used to lyse cells under non-denaturing conditions.
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RIPA Lysis Buffer, Rockland Immunochemicals
Supplier: Rockland Immunochemical
RIPA (Radio-Immunoprecipitation Assay) Lysis Buffer enables rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells.
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ReadiUse™ Viral RNA Lysis Buffer, AAT Bioquest
Supplier: AAT BIOQUEST INC
Current demands for RT-PCR testing of SARS-CoV-2 has led to a worldwide shortage of RNA extraction kits.
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Monarch® RBC Lysis Buffer, New England Biolabs
Supplier: New England Biolabs (NEB)
The Monarch® RBC lysis buffer is specifically designed to lyse red blood cells (erythrocytes) while leaving leukocytes intact. The separate RBC lysis step enables removal of hemoglobin to increase the purity of the blood prep. The RBC Lysis Buffer also removes nucleases to enable the subsequent purification of HMW DNA into the megabase (Mb) range in size.
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RBC Lysis Buffer (10X), Biolegend
Supplier: Biolegend
Red Blood Cell (RBC) Lysis Buffer has been designed, formulated, and tested to ensure optimal lysis of RBCs in single cell suspensions with minimal effects on leukocytes.
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RBC Lysis Buffer, IBI Scientific
Supplier: IBI Scientific
RBC Lysis Buffer is used to lyse red blood cells in nucleic acid isolation applications.