Blocking Buffers
The electrophoresis buffers maintain consistent pH levels during DNA, RNA, and other protein separation. Providing ions that transfer the applied current, the low charge solutions facilitate different molecular sizes to travel at various rates for analyses. Offering additional protection to proteins, the preparative substances prevent degradation for guaranteed sample viability and accurate testing. Available in various molecular weights, the best electrophoresis buffer can be selected based on the specific macromolecules being handled.
VWR® Blocking Buffers
Supplier: VWR
Most routinely used blocking buffers in a variety of formats for convenience.
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VWR® Rapidblock™ Blocking Solution, 10X
Supplier: VWR
Solution reduces blocking time to 5 minutes for Western and dot blotting procedures on PVDF and nitrocellulose membranes.
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Chemi Blot Blocking Buffer, Azure
Supplier: Azure Biosystems
Azure Chemi Blot Blocking Buffer, Ready-to-use block that enhances chemiluminescent signal, for use with chemiluminescent Western blots to enhance signal and lower background. Size: 500mL
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Azure Fluorescent Blot Blocking Buffer
Supplier: Azure Biosystems
Blocking buffer optimized for use with Azure’s fluorescent (and chemiluminescent) Western blotting systems.
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10X Blocking Buffer, Abcam
Supplier: Abcam
10X blocking buffer (pH 7.5) for serum-free blocking of non-specific antibody binding. - Cited in over 30 publications - Suitable for ELISA, in-cell ELISA, ICC/IF, western blot (WB) - 100% casein blocking as preservative
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Streptavidin Blocker/Diluent, Enzo Life Sciences
Supplier: Enzo Life Sciences
Ready-to-use blocking buffer and diluent, for use with SAVIEW® Plus detection reagents.
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BSA Blocking Buffer, 3% in TBS, with 0.05% Tween-20, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Blocking Reagents, BLOTTO and Tween 20 in PBS, with 0.02% Sodium Azide, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Blocking reagent for immunohistochemistry experiments.
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Tween 20 Blocking Buffer, 1% in PBS (10X), Thermo Scientific
Supplier: Thermo Scientific Chemicals
Viscous liquid
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BSA Blocking Buffer, 3% in PBS, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Blocking Reagents, Denhardt's Solution (100X), Thermo Scientific
Supplier: Thermo Scientific Chemicals
Denhardt's solution is used as a blocking reagent for northern or southern blots to prevent probe binding on open membrane space and non-target nucleic acids.
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BSA Blocking Buffer, 5% in TBS, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Casein Blocking Buffer, 3% in TBS, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Pierce™ Clear Milk Blocking Buffer (10X), Thermo Scientific
Supplier: Thermo Scientific
Thermo Scientific Pierce Clear Milk Blocking Buffer (10X) is a long shelf-life, high performance replacement for homemade milk blocking buffers in Western blotting applications.
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Blocking Ragents, Denhardt's Solution (50X), Thermo Scientific
Supplier: Thermo Scientific Chemicals
Denhardt's solution is used to reduce non-specific binding of detection reagents to the northern and southern blot membranes.
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BSA Blocking Buffer, 5% in PBS, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid