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241 results for Vectors, Plasmids and Libraries

You searched for: Vectors, Plasmids and Libraries

Vectors, Plasmids and Libraries

In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.

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West Nile Virus (WNV) Reporter Virus Particle (RVP) with Renilla Luciferase Reporter

West Nile Virus (WNV) Reporter Virus Particle (RVP) with Renilla Luciferase Reporter

Supplier: ReVacc Scientific

Reporter virus particles (RVPs) package the capsid-preMembrane-envelope (C-prM-E) gene construct of West Nile Virus (WNV) (GenBank: DQ211652.1, NY99 strain) and a Renilla luciferase reporter. As the wild typed WNV is handled under BSL-2 precautions, this WNV RVP can be handled using BSL-2 containment practices.

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M13K07 Helper Phage, New England Biolabs

M13K07 Helper Phage, New England Biolabs

Supplier: New England Biolabs (NEB)

M13KO7 is an M13 derivative which carries the mutation Met40Ile in gII, with the origin of replication from P15A and the kanamycin resistance gene from Tn903 both inserted within the M13 origin of replication

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pNL1.1.TK[Nluc/TK] Vector, 20 µg, Promega

pNL1.1.TK[Nluc/TK] Vector, 20 µg, Promega

Supplier: Promega Corporation

The promoter-driven NanoLuc (Nluc) control vectors can be used to co-transfect with experimental firefly luciferase vectors when using the Nano-Glo Dual-Luciferase Reporter (NanoDLR) Assay System.

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pGEM-5Zf(+) Vector, 20 µg, Promega

pGEM-5Zf(+) Vector, 20 µg, Promega

Supplier: Promega Corporation

Used as a standard cloning vector and as a template for in vitro transcription.

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Dengue Virus Reporter Virus Particle (RVP) with Renilla Luciferase Reporter (Type 1)

Dengue Virus Reporter Virus Particle (RVP) with Renilla Luciferase Reporter (Type 1)

Supplier: ReVacc Scientific

Reporter virus particles (RVPs) package the capsid-preMembrane-envelope (C-prM-E) gene construct of Dengue virus (DENV) (GenBank: AAB70694.1, SEROTYPE 1 strain) and a Renilla luciferase reporter. As the wild typed Dengue virus is handled under BSL-2 precautions, this Dengue virus RVP can be handled using BSL-2 containment practices.

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pGEM-7Zf(+) Vector, 20 µg, Promega

pGEM-7Zf(+) Vector, 20 µg, Promega

Supplier: Promega Corporation

Used as a standard cloning vector, a template for in vitro transcription or for production of circular ssDNA.

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pNL1.2[NlucP] Vector, 20 µg, Promega

pNL1.2[NlucP] Vector, 20 µg, Promega

Supplier: Promega Corporation

NanoLuc (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter. The pNL1.1[Nluc] and pNL1.2[NlucP] Vectors are used to clone putative promoter regions to express the bright NanoLuc(R) luciferase.

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pHTN HaloTag CMV-neo Vector, 20 µg, Promega

pHTN HaloTag CMV-neo Vector, 20 µg, Promega

Supplier: Promega Corporation

Vectors designed for expression of N-terminal-tagged HaloTag fusion proteins in mammalian cells. Once expressed, use the HaloTag fusion protein for cell imaging of protein localization or trafficking in conjunction with the fluorescent HaloTag Ligands.

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pFC30K His6HaloTag T7 Flexi Vector, 20 µg, Promega

pFC30K His6HaloTag T7 Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

These vectors are used for inducible expression of polyhistidine-HaloTag fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Use the Flexi Cloning System vectors for C- or N-terminal fusions.

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pNL2.2[NlucP/Hygro] Vector, 20 µg, Promega

pNL2.2[NlucP/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

NanoLuc (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter. pNL2.1[Nluc/Hygro] and pNL2.2[NlucP/Hygro] Vectors are used for cloning putative promoters; select for stable cell lines using hygromycin.

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pNL1.1.CMV[Nluc/CMV] Vector, 20 µg, Promega

pNL1.1.CMV[Nluc/CMV] Vector, 20 µg, Promega

Supplier: Promega Corporation

The promoter-driven NanoLuc (Nluc) control vectors can be used to co-transfect with experimental firefly luciferase vectors when using the Nano-Glo Dual-Luciferase Reporter (NanoDLR) Assay System.

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pGL4.38[luc2P/p53 RE/Hygro] Vector, 20 µg, Promega

pGL4.38[luc2P/p53 RE/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

These seven reporter vectors contain response elements (ARE, p53 RE, AT6 RE, MRE, HSE, HRE and XRE) that are important in various stress signaling pathways, the firefly luciferase gene and hygromycin resistance for selection in cells.

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pGL4.37[luc2P/ARE/Hygro] Vector, 20 µg, Promega

pGL4.37[luc2P/ARE/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

These seven reporter vectors contain response elements (ARE, p53 RE, AT6 RE, MRE, HSE, HRE and XRE) that are important in various stress signaling pathways, the firefly luciferase gene and hygromycin resistance for selection in cells.

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pGL4.44[luc2P/AP1 RE/Hygro] Vector, 20 µg, Promega

pGL4.44[luc2P/AP1 RE/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

pGL4.44[luc2P/AP1 RE/Hygro], pGL4.49[luc2P/TCF-LEF RE/Hygro], pGL4.29[luc2P/CRE/Hygro], pGL4.30[luc2P/NFAT-RE/Hygro], pGL4.32[luc2P/NF-κB-RE/Hygro], pGL4.33[luc2P/SRE/Hygro] and pGL4.34[luc2P/SRF-RE/Hygro] Vectors can explore various signaling pathways.

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pGL4.50[luc2/CMV/Hygro] Vector, 20 µg, Promega

pGL4.50[luc2/CMV/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

pGL4 Luciferase Reporter Vectors are the next generation of luciferase reporter vectors optimized for expression in mammalian cells.

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pFC14K HaloTag CMV Flexi Vector, 20 µg, Promega

pFC14K HaloTag CMV Flexi Vector, 20 µg, Promega

Supplier: Promega Corporation

Vectors designed for expression of C-terminal-tagged HaloTag fusion proteins in mammalian cells. Once expressed, use the HaloTag fusion protein for cell imaging of protein localization or trafficking in conjunction with the fluorescent HaloTag Ligands.

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pSP73 Vector, 20 µg, Promega

pSP73 Vector, 20 µg, Promega

Supplier: Promega Corporation

Used as standard cloning vector or for transcription of RNA in vitro.

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pGL4.25[luc2CP/minP] Vector, 20 µg, Promega

pGL4.25[luc2CP/minP] Vector, 20 µg, Promega

Supplier: Promega Corporation

pGL4.23[luc2/minP], pGL4.24[luc2P/minP] and pGL4.25[luc2CP/minP] Vectors offer different firefly luciferase protein stabilities for expression in mammalian cells. Clone a response element of interest upstream of the minimal promoter and reporter gene.

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pGL4.16[luc2CP/Hygro] Vector, 20 µg, Promega

pGL4.16[luc2CP/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

The pGL4.14[luc2/Hygro], pGL4.15[luc2P/Hygro] and pGL4.16[luc2CP/Hygro] Vectors have different luc2 genes that code for luciferase proteins with different stabilities. Each vector offers hygromycin for stable selection.

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VSV-Pseudovirus_Marburg Ravn Luciferase, ReVacc Scientific

VSV-Pseudovirus_Marburg Ravn Luciferase, ReVacc Scientific

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant Vesicular Stomatitis Virus (rVSV) to carry GP protein of Marburgvirus - Ravn (GenBank: DQ447649.1).

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VSV-Pseudovirus_SARS-CoV Luciferase, ReVacc Scientific

VSV-Pseudovirus_SARS-CoV Luciferase, ReVacc Scientific

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant Vesicular Stomatitis Virus (rVSV) to carry S protein of SARS-CoV (GenBank: AY278554.2) (2003 strain), with 28-aa cytoplasmic tail truncation for optimal infection.

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VSV-Pseudovirus_SARS-CoV-2 Beta Luciferase, ReVacc Scientific

VSV-Pseudovirus_SARS-CoV-2 Beta Luciferase, ReVacc Scientific

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant Vesicular Stomatitis Virus (rVSV) to carry spike protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations identified in Beta variant: L18F, D80A, D215G, L242-244del, R246I, K417N, E484K, N501Y, D614G, and A701V. The spike protein has a 18-aa cytoplasmic tail truncation for optimal infection.

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sparQ mRNA-Seq Kit

sparQ mRNA-Seq Kit

Supplier: Quantabio

Ultra-sensitive mRNA library prep workflow with efficient poly(A) capture.

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VSV-Pseudovirus_HCV H77 Strain Luciferase

VSV-Pseudovirus_HCV H77 Strain Luciferase

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the full length E1E2 of Hepatitis C virus (HCV) (H77 strain) (GenBank: AAB67036.1).

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pGL4.21[luc2P/Puro] Vector, 20 µg, Promega

pGL4.21[luc2P/Puro] Vector, 20 µg, Promega

Supplier: Promega Corporation

The pGL4.20[luc2/Puro], pGL4.21[luc2P/Puro] and pGL4.22[luc2CP/Puro] Vectors are optimized for expression in mammalian cells. The luc2 genes code for luciferase proteins with different stabilities, and each vector offers puromycin for stable selection.

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pGL4.36[luc2P/MMTV/Hygro] Vector, 20 µg, Promega

pGL4.36[luc2P/MMTV/Hygro] Vector, 20 µg, Promega

Supplier: Promega Corporation

NR ligand binding domain fused to yeast GAL4 TF DBD in the FN26A (BIND) Flexi Vector or use pBIND-Er∝ and pBIND-GR Vectors. Fusion protein NR activates luc2P reporter controlled by 9X GAL4 UAS in pGL4.35. Use pGL4.36 MMTV LTR for androgen or GC responses.

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VSV-Pseudovirus_Zaire Ebola Luciferase, ReVacc Scientific

VSV-Pseudovirus_Zaire Ebola Luciferase, ReVacc Scientific

Supplier: ReVacc Scientific

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry GP protein of Zaire ebolavirus Makona strain (GenBank: KR781609.1).

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PH.D.™ Peptide Display Cloning System, New England Biolabs

PH.D.™ Peptide Display Cloning System, New England Biolabs

Supplier: New England Biolabs (NEB)

The Ph.D. Peptide ligands for a variety of targets can then be selected by the straightforward method of panning.

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Lentiviral Control Vectors, Cell Biolabs

Lentiviral Control Vectors, Cell Biolabs

Supplier: Cell Biolabs

Cell Biolabs' Lentiviral Control Plasmids are constructs containing one of a variety of reporter genes

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AAV-6 Helper Free Expression Complete Systems, Cell Biolabs

AAV-6 Helper Free Expression Complete Systems, Cell Biolabs

Supplier: Cell Biolabs

AAV-6 Helper Free Expression Complete Systems contain the following constructs needed to package your recombinant serotype-6 adeno-associated virus: an expression vector, pHelper plasmid, a serotype-specific Rep-Cap plasmid, and an optional GFP expression vector for making a control virus

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