Vectors, Plasmids and Libraries
In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.
sparQ mRNA-Seq Kit
Supplier: Quantabio
Ultra-sensitive mRNA library prep workflow with efficient poly(A) capture.
Expand 2 Items
Polyadenylic acid (poly-A)
Supplier: Cytiva
Poly(A) (polyadenylic acid) can be used to evaluate the binding of potential therapeutic agents to mRNA in drug development research.
Expand 2 Items
AAV Helper-Free System, Agilent Technologies
Supplier: Agilent Technologies
The AAV Helper-Free System eliminates the requirement for wild-type adenovirus co-infection from both AAV vector production and AAV stock titering steps, making this system entirely helper virus-free and safer. This system works with a broad range of hosts and infects both dividing and non-dividing cells.
Expand 2 Items
InterPlay Adenoviral TAP System, Agilent Technologies
Supplier: Agilent Technologies
The InterPlay Mammalian TAP system allows you to recover interacting proteins from mammalian cells.
Expand 3 Items
Vector, pUC 18, MP Biomedicals
Supplier: MP Biomedicals
pUC 18 is a small vector of approximately 2.7 Kb containing the Pvu II/EcoR I fragment of pBR322 and the lacZ gene multiple cloning site from M13mp18.
Expand 1 Items
InterPlay Adenoviral TAP Systems, Agilent Technologies
Supplier: Agilent Technologies
The InterPlay Adenoviral TAP System combines our unique tandem affinity purification (TAP) system with our exclusive adenoviral gene delivery system, the AdEasy Adenoviral Vector System, for enhanced gene delivery to a broader range of mammalian cells, higher protein yields, and improved purification and analysis of endogenous interacting protein partners.
Expand 2 Items
SureVector, Vectors, Agilent Technologies
Supplier: Agilent Technologies
Combine standard DNA components to build custom vector constructs from Agilent’s set of standard parts.
Expand 3 Items
Vector, E. coli BL21, Cytiva
Supplier: Cytiva
The E. coli BL21 strain is protease-deficient for optimal expression of a variety of recombinant proteins.
Expand 1 Items
AdEasy Adenoviral Vector Systems, Agilent Technologies
Supplier: Agilent Technologies
The AdEasy system saves you a month of work over traditional methods by producing the recombinant adenoviral plasmid by homologous recombination in E. coli. The AdEasy XL system includes BJ5183 cells pre-transformed with the pAdEasy-1 plasmid, this feature dramatically decreases background caused by the non-recombinant shuttle plasmid.
Expand 9 Items
pBlueScript II Vectors, Agilent Technologies
Supplier: Agilent Technologies
The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping.
Expand 3 Items
Expression vectors, pGEX
Supplier: Cytiva
The pGEX vectors have an expanded multiple cloning site (MCS) that contains six restriction sites. The expanded MCS facilitates the unidirectional cloning of cDNA inserts obtained from libraries constructed using many available lambda vectors. pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3 each encode the recognition sequence for site-specific cleavage by PreScission Protease between the GST domain and the multiple cloning site. pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3 are derived from pGEX-2T and contain a Thrombin recognition site. pGEX-5X-1, pGEX-5X-2, and pGEX5X-3 are derivatives of pGEX-3X and possess a Factor Xa recognition site.
Expand 6 Items
AdEasy Adenoviral Vector Systems, Agilent Technologies
Supplier: Agilent Technologies
The AAV helper-free system eliminates the requirement for wild-type adenovirus co-infection from both AAV vector production and AAV stock titering steps, making this system entirely helper virus-free and safer.
Expand 3 Items
pJY2 Plasmid, Enzo Life Sciences
Supplier: Enzo Life Sciences
pJY2 encodes tRNAUCUArg to suppress lysine misincorporation at AGA codons and T7 lysozyme to depress constitutive expression of the cloned gene. pJY2 can also be used as a source of tRNAUCUArg with expression systems, such as the pGEX series of vectors, that do not rely on T7 polymerase.
Expand 1 Items
VSV-Pseudovirus_SARS-CoV-2 Delta Luciferase, ReVacc Scientific
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of B.1.617.2 (Delta strain), and 18-aa cytoplasmic tail truncation for optimal infection.
Expand 2 Items
pGL4.73[hRluc/SV40] Vector, 20 µg, Promega
Supplier: Promega Corporation
pGL4.73[hRluc/SV40], pGL4.74[hRluc/TK] and pGL4.75[hRluc/CMV] Vectors are used as the control reporter for dual-luciferase assays. Select the appropriate promoter or test all three in your experimental system.
Expand 1 Items
pGL4.18[luc2P/Neo] Vector, 20 µg, Promega
Supplier: Promega Corporation
The pGL4.17[luc2/Neo] Vector, pGL4.18[luc2P/Neo] and pGL4.19[luc2CP/Neo] Vectors are optimized for expression in mammalian cells. These vectors offer standard and destablized luciferase with neomycin for stable selection.