You searched for: qPCR Assays
qPCR assays are used for many applications, including gene expression analysis, microRNA analysis, single nucleotide polymorphism, genotyping, copy number variation analysis, and even protein analysis. These assays are easy to implement and can be employed for both relative and absolute quantification methods. Successful qPCR assays require efficient and specific amplification of the product. A range of sample volumes can be tested simultaneously, with options to test multiple well-plates simultaneously in some models.
Crystal Digital PCR® Assay for BRAF/KRAS/NRAS
Supplier: STILLA TECHNOLOGIES, INC.
Assay-specific crystal flex probes and primers for the detection of BRAF/KRAS/NRAS mutations and its positive control. The BRAF/KRAS/NRAS Crystal Digital PCR® assay is a 10X assay designed to detect and quantify mutations in BRAF, KRAS, and NRAS genes using the ruby chip. BRAF, KRAS, and NRAS genes are pivotal in regulating cell signaling pathways implicated in cancer development.
Expand 2 Items
Crystal Digital PCR® Assay for EGFR (drop-off Del19)
Supplier: STILLA TECHNOLOGIES, INC.
Assay-specific Crystal Flex Probes and primers for the detection of EGFR mutations and its Positive Control. The EGFR (drop-off Del19) Crystal Digital PCR® Assay is a 10X assay designed to detect and quantify EGFR Exon 19 deletion mutations using the Ruby Chip. EGFR encodes for the epidermal growth factor receptor, a protein that plays a crucial role in cell growth, proliferation, and differentiation.
Expand 2 Items
Crystal Digital PCR® Assay for KRAS (G12C, drop-off G12-G13)
Supplier: STILLA TECHNOLOGIES, INC.
Assay-specific crystal flex probes and primers for the detection of KRAS mutations and its positive control. The KRAS (G12C, drop-off G12-G13) Crystal Digital PCR® assay is a 10X assay designed to detect and quantify mutations in codons 12 and 13 plus one mutation of the KRAS gene. KRAS is pivotal in regulating cell signaling pathways implicated in cancer development, notably melanoma and colorectal cancer.
Expand 2 Items
Crystal Digital PCR® Assay for EGFR (L858, L858R, L861Q)
Supplier: STILLA TECHNOLOGIES, INC.
Assay-specific Crystal Flex Probes and primers for the detection of EGFR mutations and its Positive Control. The EGFR (L858, L858R, L861Q) Crystal Digital PCR® Assay is a 10X assay designed to detect and quantify 2 mutations in the EGFR gene using the Ruby Chip. EGFR encodes for the epidermal growth factor receptor, a protein that plays a crucial role in cell growth, proliferation, and differentiation.
Expand 2 Items
Crystal Digital PCR® Assay for C. albicans
Supplier: STILLA TECHNOLOGIES, INC.
Assay-specific Crystal Flex Probes and primers for the detection of C. albicans and its Positive Control. C. albicans Crystal Digital PCR® Assay is a 10X assay designed to detect and quantify 16 genes (1 gene per chromosome) of Candida albicans using the Ruby Chip. Candida albicans is a common yeast within the human gastrointestinal microbiota. Under certain conditions, it can become pathogenic leading to infections.
Expand 2 Items
Crystal Digital PCR® Assay for c-KIT (D816-D816V)
Supplier: STILLA TECHNOLOGIES, INC.
Assay-specific crystal flex probes and primers for the detection of c-KIT mutations and its positive control. The c-KIT (D816, D816V) Crystal Digital PCR® Assay is a 10X assay designed to detect and quantify 1 mutation in the c-KIT gene using the Ruby Chip. c-KIT encodes for a receptor tyrosine kinase that plays a crucial role in cell signaling for processes including cell survival, proliferation, and differentiation.
Expand 2 Items
Crystal Digital PCR® Assay for MYD88 (L265, L265P)
Supplier: STILLA TECHNOLOGIES, INC.
Assay-specific Crystal Flex Probes and primers for the detection of MYD88 mutations and its positive control. The MYD88 (L265, L265P) Crystal Digital PCR® Assay is a 10X assay designed to detect and quantify 1 mutation in the MYD88 gene using the Ruby Chip. MYD88 encodes the MYD88 protein, which is an adaptor protein essential for transmitting signals in the immune system, mediating responses to infections, and modulating inflammation and cell survival.
Expand 2 Items
Crystal Digital PCR® Assay for TP53 (drop-off V272-A276/G280-R283)
Supplier: STILLA TECHNOLOGIES, INC.
Assay-specific Crystal Flex Probes and primers for the detection of TP53 mutations and its Positive Control. TP53 (drop-off V272-A276/G280-R283) Crystal Digital PCR® Assay is a 10X assay designed to detect and quantify mutations between V272-A276 or G280-R283 of the TP53 gene using the Ruby Chip. TP53 is pivotal in regulating cell divisison, thus an important tumor suppressor gene.
Expand 2 Items
Crystal Digital PCR® Assay for KRAS (G12A, G12C, G12D, G12V, drop-off G12-G13)
Supplier: STILLA TECHNOLOGIES, INC.
Assay-specific crystal flex probes and primers for the detection of KRAS mutations and its positive control. The KRAS (G12A, G12C, G12D, G12V, drop-off G12-G13) Crystal Digital PCR® assay is a 10X assay designed to detect and quantify mutations in codons 12 and 13 plus 4 mutations in the KRAS gene using the ruby chip. KRAS is pivotal in regulating cell signaling pathways implicated in cancer development, notably melanoma and colorectal cancer.
Expand 2 Items
Crystal Digital PCR® Assay for BRCA1/RAD51C/GSTP1 Methylation
Supplier: STILLA TECHNOLOGIES, INC.
Assay-specific crystal flex probes and primers for the detection of BRCA1/RAD51C/GSTP1 methylation and its positive control. The BRCA1/RAD51C/GSTP1 methylation Crystal Digital PCR® assay is a 10X assay designed to detect and quantify methylation of the BRCA1, RAD51C, and GSTP1 promoter using the ruby chip. Methylation of the BRCA1, RAD51C, and GSTP1 affects DNA repair mechanisms crucial for genome stability and dysregulation of detoxification pathways, both contributing to tumor initiation, progression, and drug resistance.
Expand 2 Items
Crystal Digital PCR® Assay for TP53 (drop-off Y205-R209/V218-E221)
Supplier: STILLA TECHNOLOGIES, INC.
Assay-specific Crystal Flex Probes and primers for the detection of TP53 mutations and its positive control. TP53 (drop-off Y205-R209/V218-E221) Crystal Digital PCR® Assay is a 10X assay designed to detect and quantify mutations between Y205-R209 or V218-E221 of the TP53 gene using the Ruby Chip. TP53 is pivotal in regulating cell divisison, thus an important tumor suppressor gene.
Expand 2 Items
Myco-Sniff-Valid™ Mycoplasma PCR Kit, MP Biomedicals
Supplier: MP Biomedicals
Myco-Sniff-Valid™ mycoplasma PCR detection it provides a highly sensitive, specific and rapid method for the detection of a broad range of mycoplasma species in cell cultures.
Expand 1 Items
Myco-Sniff™ Mycoplasma PCR Kit, MP Biomedicals
Supplier: MP Biomedicals
Myco-Sniff™ mycoplasma PCR detection kit provides a highly sensitive, specific and rapid method for the detection of a broad range of mycoplasma species in cell cultures.
Expand 1 Items
ROX Reference Dye, Biotium
Supplier: Biotium
ROX reference dye is used to normalize well-to-well variations that may occur during quantitative, real-time PCR reactions.
Expand 1 Items
ARM-D Kit, ampC (RUO), Streck
Supplier: Streck Laboratories
The Streck ARM-D Kit, AmpC, is a comprehensive, TaqMan based method for the detection of plasmid based antibiotic resistance gene families in Gram Negative Bacteria.
Expand 1 Items
QuantiFluor® dsDNA System, Promega
Supplier: Promega Corporation
The QuantiFluor dsDNA System contains a fluorescent DNA-binding dye that enables sensitive quantitation of small amounts of double-stranded DNA (dsDNA) in solution. The dye shows minimal binding to single stranded DNA (ssDNA) and RNA.
Expand 2 Items
Sunnyvale Red™ SE *Superior 6-ROX Replacement*, AAT Bioquest
Supplier: AAT BIOQUEST INC
Although ROX dyes have strong fluorescence they are notoriously unstable.
Expand 1 Items
6-ROXtra™ SE Superior 6-ROX Replacement, AAT BIOQUEST INC
Supplier: AAT BIOQUEST INC
Although ROX dyes have strong fluorescence they are notoriously unstable.
Expand 2 Items
ARM-D Kit, β-Lactamase, Steck
Supplier: Streck Laboratories
The Streck ARM-D Kit, β-Lactamase, is a comprehensive, TaqMan based method for the detection of antibiotic resistance gene families in Gram Negative Bacteria, including the five CRE's described by the CDC as urgent public health threats.
Expand 1 Items
Helixyte™ Green *10000X Aqueous PCR Solution*, AAT Bioquest
Supplier: AAT BIOQUEST INC
Helixyte™ Green is manufactured by AAT Bioquest, and it has the same chemical structure of SYBR® Green (SYBR® is the trademark of ThermoFisher).
Expand 1 Items
6-ROXtra™ Fluorescence Reference Solution *25 µM for PCR Reactions*, AAT Bioquest
Supplier: AAT BIOQUEST INC
6-ROX is predominately used as a reference dye for performing PCR detections.
Expand 1 Items
Rt-Qpcr Kit Detect Covid-19, Intact Genomics
Supplier: INTACT GENOMICS, INC MS
Intact Genomics COVID-19 or SARS-CoV-2 Coronavirus detection kit is used for in vitro detection of SARS-CoV-2 using real-time quantitative PCR (RT-qPCR). For research use only.
Expand 2 Items
MutaPLATE® HLA DQ2+8 (TM) PCR Assay Kit, Eagle Biosciences
Supplier: Eagle Biosciences
MutaPLATE® HLA-DQ2+8 (TM) real time PCR kit is a molecular biological test for detection of the genetic profile determining the HLA class II serotypes DQ2 and DQ8 (MHC system) (LPH) in open real time PCR systems.
Expand 2 Items
FastAmp® Solution Covid-19 Test, Intact Genomics
Supplier: INTACT GENOMICS, INC MS
FastAmp® viral and cell solution for Covid-19 streamlines viral specimen collection and viral RNA preparation processes including sample collection, transport, maintenance, viral/cell lysis, and viral RNA stabilization.
Expand 2 Items
Helixyte™ Green *20X Aqueous PCR Solution*, AAT Bioquest
Supplier: AAT BIOQUEST INC
Helixyte™ Green is manufactured by AAT Bioquest, and it has the same chemical structure of SYBR® Green (SYBR® is the trademark of ThermoFisher).
Expand 1 Items
ROX Reference Dye *50X Fluorescence Reference Solution for PCR Reactions*, AAT Bioquest
Supplier: AAT BIOQUEST INC
ROX Reference Dye is equivalent to 12223-012 (Life Technology).