Pierce™ PITC, Edmans Reagent, Thermo Scientific

Catalog # PI26922

Supplier: Invitrogen

CAS Number:

SDS

Certificates

Pierce™ PITC, Edmans Reagent, Thermo Scientific

Catalog # PI26922

Supplier: Invitrogen

Supplier Number: 26922

CAS Number:

SDS

Certificates

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Specifications

Description:
PITC (Edman’s reagent)
Size:
10 x 1 mL
Cat. No.:
PI26922
Supplier no.:
26922

Specifications

About this item

This Phenylisothiocyanate (PITC) is a high-purity reagent for pre-column quantitative derivatization of amino acids by reverse-phase HPLC.

For pre-column derivatization of amino acids for picomole detection levels in HPLC
Reacts with amine groups of amino acids in 5 to 10 minutes at room temperature to yield products that are detectable by UV absorbance (λmax = 254nm)
Produces phenylthiocarbamyl (PTC) derivatives that can be separated and quantified in 30 minutes using reverse-phase HPLC
Produces stable products with all amino acids, including proline

Phenylisothiocyanate (PITC), also known as Edman's Reagent, enables the sequential degradation of amino acids in a polypeptide chain, yielding primary structural information.

Reagent Properties:
Alternative names: PITC, Phenyl Isothiocyanate, Edman's Reagent
Chemical name: Isothiocyanatobenzene
Reactive toward: Primary amines (—NH2)
Chemical formula: C₇H₅NS
CAS number: 103-72-0
Molecular weight: 135.19
Form: Clear, colorless to pale yellow liquid
Storage: Upon receipt store at room temperature

Unlike Fmoc-chloride, PITC does not yield disubstituted tyrosine or histidine derivatives. PTC-amino acids demonstrate improved stability at pH 5-7.5 as well as increased stability at room temperature over o-phthalaldehyde (OPA)-amino acid adducts. Also, unlike OPA, PITC enables the direct analysis of secondary amino acids.

Caution: For Research Use Only. Not for use in diagnostic procedures.