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Specifications
- Assay duration:Multiple steps
- Assay Type (ELISA with LOV):Sandwich
- Conjugate ELISA:Biotin
- Format:Pre-coated
- Host:Rabbit
- Primary antibody reactivity:Human
- Target protein:GLO1
- Size:1 kit
- Sample Type:Tissue homogenates, cell lysates and other biological fluids
- Cross Reactivity:No significant cross-reactivity or interference between Glyoxalase I (GLO1) and analogues was observed
- Detection Method:Colorimetric
- Time to Results:3 h
- Shelf Life:12 Months
- Detection Range:1.56 - 100 ng/ml
- Storage Temperature:4 °C for one month (frequent use), −20 °C for one year
- Sample Volume:100 µl
- Sensitivity:0.59 ng/ml
- Regulatory Status:RUO
- Cat. No.:MSPP-SEC501HU
- No. of tests:96 wells
Specifications
About this item
This assay has high sensitivity and excellent specificity for detecting Human GLO1 (Glyoxalase I). The assay range is from 1.56 to 100 ng/ml (Sandwich kit) with a sensitivity of 0.59 ng/ml. There is no detectable cross-reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.
- High sensitivity and specificity
- Perfect reproducibility and consistency across batches
- Quality control with three-level inspections
- Wide range of targets/species available
- Intra-assay: CV<10%; Inter-assay: CV<12%
Glyoxalase I (EC 4.4.1.5) is a glutathione-binding protein involved in the detoxification of methylglyoxal, a byproduct of glycolysis. GLO1 and glyoxalase II (GLO2) catalyze successive steps in the pathway. GLO1 catalyzes condensation of methylglyoxal and reduced glutathione to form S-lactoyl-glutathione; GLO2 (hydroxyacyl glutathione hydrolase) converts the latter substance to D-lactic acid and reduced glutathione. The human enzyme showed 42% amino acid homology with bacterial Glo1. Northern blot analysis identified a 2.2 kb mRNA transcript in colon tissue. There was a 12-fold increase of the GLO1 transcript in colon carcinoma tissue compared to normal colon tissue from the same patient, and the authors concluded that GLO1 gene expression was induced in colon carcinoma.