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Specifications
- Description:STEMdiff™ T cell kit
- Size:1 kit
- Environmentally Preferable:
- Cat. No.:MSPP-100-0194
Specifications
About this item
For expansion and differentiation of hPSCs to T cells.
Differentiate human pluripotent stem cells (hPSCs) to T cells with the feeder-free and serum-free STEMdiff™ T cell kit. The STEMdiff™ T Cell Kit protocol first uses animal component-free STEMdiff™ Hematopoietic - EB reagents for the generation of embryoid bodies (EBs) from hPSCs which are then further differentiated into CD34+ cells.
The kit includes all the EB reagents necessary for this step:
STEMdiff™ hematopoietic - EB basal medium
STEMdiff™ hematopoietic - EB supplement A
STEMdiff™ hematopoietic - EB supplement B
The CD34+ cells are then further directed to differentiate to T cells using StemSpan™ reagents:
StemSpan™ SFEM II
StemSpan™ lymphoid progenitor expansion supplement (10X)
StemSpan™ lymphoid differentiation coating material (100X)
StemSpan™ T cell progenitor maturation supplement (10X)
For convenience, all STEMdiff™ Hematopoietic - EB and StemSpan™ reagents included with the kit are also available individually.