Cell Culture Buffers
Tissue culture buffers provide an energy source, maintain proper pH levels, and keep ideal osmotic pressure present. Used predominately in biomolecular research, the dry and liquid substances have a balanced inorganic salt composition. Lower concentration options permit equilibration with air in a closed system, while higher salt concentration options keep gaseous phases in equilibrium. Cell growth is optimal when plated in a nutritional medium that has been buffered.
VWR® Sodium Phosphate 0.1 M, pH 7.0 Buffer
Supplier: VWR International
Made with sodium phosphate monobasic monohydrate, sodium hydroxide solution and deionized water.
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VWR® Phosphate Buffered Saline (PBS)
Supplier: VWR International
Balanced salt solution used for multiple cell culture applications.
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VWR® Blood Bank Saline Solutions
Supplier: VWR International
Blood bank saline is pH-controlled for a pH between 6.5 and 7.0.
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VWR® Dulbecco's Phosphate Buffered Saline Solutions
Supplier: VWR International
Balanced salt solution used for multiple cell culture applications, (liquid form).
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VWR® Hanks' Balanced Salt Solutions
Supplier: VWR International
Balanced salt solutions for cell culture applications.
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VWR® Phosphate Buffer Solution
Supplier: VWR
Phosphate Buffered Saline (PBS) with 0.05% Tween® 20, pH 7.5 is a ready-to-use 1X concentration
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VWR® Phosphate Buffered Saline (PBS), 10X, Ultra Pure Grade
Supplier: VWR
1X Solution Composition: 137 mM NaCl, 2.7 mM KCl, 9.5 mM Phosphate buffer.
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VWR® TRIS Buffer, 1 M Solution, pH 8.0
Supplier: VWR
Tris (hydroxymethyl) aminomethane
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VWR® Ultra Pure Grade Phosphate Buffered Saline
Supplier: VWR
1X Solution Composition:137 mM NaCl, 2.7 mM KCl, 9.5 mM Phosphate buffer
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VWR® TRIS Buffer, 0.1 M Solution, pH 7.4
Supplier: VWR
Cell culture buffer, TRIS BUFFER, 0.1M SOLUTION, pH 7.4
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Phosphate Buffered Saline (PBS),Powdered, Ultra Pure Grade
Supplier: VWR
1X Solution Composition:137 mM NaCl,2.7 mM KCl,9.8 mM Phosphate buffer
May be sterilized by filtration.
Does not contain magnesium or calcium.
pH at 25°C (1X PBS): 7.3 – 7.5
Conductivity at 25°C (1X PBS): 13,500-17,000 umhos
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VWR® Phosphate Buffered Saline (PBS) 20X, Ultra Pure Grade
Supplier: VWR
1X Solution Composition:135 mM NaCl, 2.7 mM KCl,11 mM Phosphate Buffer.Does not contain magnesium or calcium.
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Phosphate Buffered Saline
Supplier: Ward's Science
Cell culture buffer, Phosphate Buffered Saline
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VWR® Phosphate Buffered Saline, Ultra Pure Grade
Supplier: VWR
1X Solution Composition: 137 mM NaCl, 2.7 mM KCl, 9.8 mM Phosphate buffer
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Corning® EDTA, 0.5M pH 8.0, Corning
Supplier: Corning
EDTA (ethylenediaminetetraacetic acid) is a polyamino carboxylic acid. It is a colorless, water-soluble solid and is useful as a chelating agent due to its ability to "sequester" metal ions such as Ca2+ and Fe3+. These cations are responsible for various undesirable effects in molecular biological and cell culture processes. After being bound by EDTA, these metal ions remain in the solution but exhibit diminished reactivity. The use of EDTA therefore allows for greater control over many experiments.
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Corning® HEPES buffer solution (1 mol/L)
Supplier: Corning
HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulphonic acid) provides an alternative to the use of bicarbonate for buffering because it does not require elevated levels of CO2
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