In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.
pGloSensor-22F cAMP Plasmid, 20 µg, Promega
Supplier: Promega Corporation
The GloSensor cAMP Assay is based on a genetically modified form of firefly luciferase into which a cAMP-binding protein moiety has been inserted. This live-cell assay excels at kinetic and modulation studies of signaling through cAMP.
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pGL4.39[luc2P/ATF6 RE/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
These seven reporter vectors contain response elements (ARE, p53 RE, AT6 RE, MRE, HSE, HRE and XRE) that are important in various stress signaling pathways, the firefly luciferase gene and hygromycin resistance for selection in cells.
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pGL4.32[luc2P/NF-kappa-B-RE/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
pGL4.44[luc2P/AP1 RE/Hygro], pGL4.49[luc2P/TCF-LEF RE/Hygro], pGL4.29[luc2P/CRE/Hygro], pGL4.30[luc2P/NFAT-RE/Hygro], pGL4.32[luc2P/NF-κB-RE/Hygro], pGL4.33[luc2P/SRE/Hygro] and pGL4.34[luc2P/SRF-RE/Hygro] Vectors can explore various signaling pathways.
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pGL4.33[luc2P/SRE/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
pGL4.44[luc2P/AP1 RE/Hygro], pGL4.49[luc2P/TCF-LEF RE/Hygro], pGL4.29[luc2P/CRE/Hygro], pGL4.30[luc2P/NFAT-RE/Hygro], pGL4.32[luc2P/NF-κB-RE/Hygro], pGL4.33[luc2P/SRE/Hygro] and pGL4.34[luc2P/SRF-RE/Hygro] Vectors can explore various signaling pathways.
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pmirGLO Dual-Luciferase miRNA Target Expression Vector, 20 µg, Promega
Supplier: Promega Corporation
The pmirGLO Vector is designed to quantitatively evaluate microRNA (miRNA) activity by the insertion of miRNA target sites downstream or 3' of the firefly luciferase gene (luc2).
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pFN21A HaloTag CMV Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
Vectors designed for expression of N-terminal-tagged HaloTag fusion proteins in mammalian cells. Once expressed, use the HaloTag fusion protein for cell imaging of protein localization or trafficking in conjunction with the fluorescent HaloTag Ligands.
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pMAL-c6T Vector, New England Biolabs
Supplier: New England Biolabs (NEB)
The pMAL-c6T Vector is designed to produce maltose-binding protein (MBP) fusions in the cytoplasm.
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pFN31K Nluc CMV-neo Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
The pFN31 and pFC32 Vectors generate N- or C-terminal fusions of NanoLuc luciferase with your protein of interest. Use pFN31A/K Nluc CMV-Hygro Flexi Vectors for N-terminal fusions and pFC32A/K Nluc CMV-Hygro Flexi Vectors for C-terminal fusions.
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pF3A WG (BYDV) Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
Designed for use with Wheat Germ extracts and contain sequences from the barley yellow dwarf virus (BYDV), an RNA plant virus, upstream and downstream of the protein coding region of interest.
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pNL3.2.CMV Vector, 20 µg, Promega
Supplier: Promega Corporation
NanoLuc (Nluc) luciferase is a small bright luminescent reporter enzyme (19.1kDa) engineered for optimal performance. Use the pNL3.2.CMV Vector as a negative control in experiments measuring regulated changes in NanoLuc luciferase expression.
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pF3K WG (BYDV) Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
Designed for use with Wheat Germ extracts and contain sequences from the barley yellow dwarf virus (BYDV), an RNA plant virus, upstream and downstream of the protein coding region of interest.
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pGL4.53[luc2/PGK] Vector, 20 µg, Promega
Supplier: Promega Corporation
pGL4.13[luc2/SV40] ,pGL4.53[luc2/PGK] and pGL4.54[luc2/TK] Vectors are used as controls in dual-luciferase assays where Renilla or NanoLuc luciferase is the experimental reporter. Select the appropriate promoter or test all 3 in your experimental system.
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pGL4.28[luc2CP/minP/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
pGL4.26[luc2/minP/Hygro], pGL4.27[luc2P/minP/Hygro] and pGL4.28[luc2CP/minP/Hygro] Vectors are used to clone a response element of interest upstream of the minimal promoter and firefly luciferase gene with and without destabilization sequences.
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pGL4.29[luc2P/CRE/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
pGL4.44[luc2P/AP1 RE/Hygro], pGL4.49[luc2P/TCF-LEF RE/Hygro], pGL4.29[luc2P/CRE/Hygro], pGL4.30[luc2P/NFAT-RE/Hygro], pGL4.32[luc2P/NF-κB-RE/Hygro], pGL4.33[luc2P/SRE/Hygro] and pGL4.34[luc2P/SRF-RE/Hygro] Vectors can explore various signaling pathways.
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pGL4.74[hRluc/TK] Vector, 20 µg, Promega
Supplier: Promega Corporation
pGL4.73[hRluc/SV40], pGL4.74[hRluc/TK] and pGL4.75[hRluc/CMV] Vectors are used as the control reporter for dual-luciferase assays. Select the appropriate promoter or test all three in your experimental system.
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pGL4.11[luc2P] Vector, 20 µg, Promega
Supplier: Promega Corporation
The pGL4.10[luc2], pGL4.11[luc2P] and pGL4.12[luc2CP] Vectors are promoterless reporter vectors optimized for expression in mammalian cells. The different luc2 genes code for luciferase proteins with different stabilities.
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VSV-Pseudovirus_SARS-CoV-2 Alpha Luciferase, ReVacc Scientific
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry spike protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations identified in variant of B.1.1.7 (Alpha strain), including: ΔH69/V70, ΔY144, N501Y, A570D, D614G, P681H, T716I, S982A, and D1118H. The spike protein has a 18-aa cytoplasmic tail truncation for optimal infection
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pNLCoI3[luc2-P2A-NlucP/CMV/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
The pNLCoI Vectors comprise a second-generation coincidence reporter vector system that allow expression of both firefly luciferase (luc2) and NanoLuc Luciferase fused to a PEST destabilization domain (NlucP) from the same mRNA transcript.
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pBR322 Vector, New England Biolabs
Supplier: New England Biolabs (NEB)
pBR322 DNA is a commonly used plasmid cloning vector in E.coli.
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VSV-Pseudovirus_SARS-COV-2 Omicron XBB.1.16 Luciferase
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron XBB.1.16. The S has 18-aa cytoplasmic tail truncation for optimal infection.
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pGL4.31[luc2P/GAL4UAS/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
The CheckMate/Flexi Vector Mammalian Two-Hybrid System provides a means to study suspected interactions between two proteins or domains and can also be used to generate stable cell lines for cell-based assays.
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pF4K CMV Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
Designed for high-level expression of proteins in mammalian cells from the CMV promoter without a selectable marker
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pFN28A HaloTag CMV-neo Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
Vectors designed for expression of N-terminal-tagged HaloTag fusion proteins in mammalian cells. Once expressed, use the HaloTag fusion protein for cell imaging of protein localization or trafficking in conjunction with the fluorescent HaloTag Ligands.
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pFN28K HaloTag CMV-neo Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
Vectors designed for expression of N-terminal-tagged HaloTag fusion proteins in mammalian cells. Once expressed, use the HaloTag fusion protein for cell imaging of protein localization or trafficking in conjunction with the fluorescent HaloTag Ligands.
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pTXB1 Vector DNA, New England Biolabs
Supplier: New England Biolabs (NEB)
pTXB1 is an E.coli expression vector in the IMPACT™ Kit.
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pNL1.3.CMV[secNluc/CMV] Vector, 20 µg, Promega
Supplier: Promega Corporation
The pNL1.3[secNluc], pNL3.3[secNluc/minP], pNL2.3[secNluc/Hygro] and pNL1.3.CMV[secNluc/CMV] Vectors offer a secreted small luciferase reporter in various promoter-driven or promoterless configurations for expression in mammalian cells.
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pGL4.40[luc2P/MRE/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
These seven reporter vectors contain response elements (ARE, p53 RE, AT6 RE, MRE, HSE, HRE and XRE) that are important in various stress signaling pathways, the firefly luciferase gene and hygromycin resistance for selection in cells.
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pGL4.42[luc2P/HRE/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
These seven reporter vectors contain response elements (ARE, p53 RE, AT6 RE, MRE, HSE, HRE and XRE) that are important in various stress signaling pathways, the firefly luciferase gene and hygromycin resistance for selection in cells.
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VSV-Pseudovirus_Marburg Angola Luciferase, ReVacc Scientific
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant Vesicular Stomatitis Virus (rVSV) to carry GP protein of Marburgvirus - Angola (GenBank: KU978782.1).
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VSV-Pseudovirus_SARS-CoV-2 Omicron BA.4/5 Strain Spike with Luciferase Reporter
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron BA.4/5 (BA.4 and BA.5 variants share the same Spike mutations). The S has 18-aa cytoplasmic tail truncation for optimal infection.