You searched for: Vectors, Plasmids and Libraries
In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.
pGloSensor-20F cAMP Plasmid, 20 µg, Promega
Supplier: Promega Corporation
The GloSensor cAMP Assay is based on a genetically modified form of firefly luciferase into which a cAMP-binding protein moiety has been inserted. This live-cell assay excels at kinetic and modulation studies of signaling through cAMP.
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pHTC HaloTag CMV-neo Vector, 20 µg, Promega
Supplier: Promega Corporation
Vectors designed for expression of N-terminal-tagged HaloTag fusion proteins in mammalian cells. Once expressed, use the HaloTag fusion protein for cell imaging of protein localization or trafficking in conjunction with the fluorescent HaloTag Ligands.
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pF9A CMV hRluc-neo Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
The Flexi Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences.
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pTNT Vector, 20 µg, Promega
Supplier: Promega Corporation
The pCMVTNT and pTNT Vectors are designed for convenient expression of cloned genes in vitro or in vivo. SP6 and T7 promoters allow expression from SP6- or T7-based coupled in vitro transcription/translation systems.
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pUC19 Vector, New England Biolabs
Supplier: New England Biolabs (NEB)
pUC19 is a commonly used plasmid cloning vector in E.coli
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M13mp18 RF I DNA, New England Biolabs
Supplier: New England Biolabs (NEB)
M13mp18 is the double-stranded, covalently closed, circular form of DNA derived from bacteriophage M13.
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West Nile Virus (WNV) Reporter Virus Particle (RVP) with Renilla Luciferase Reporter
Supplier: ReVacc Scientific
Reporter virus particles (RVPs) package the capsid-preMembrane-envelope (C-prM-E) gene construct of West Nile Virus (WNV) (GenBank: DQ211652.1, NY99 strain) and a Renilla luciferase reporter. As the wild typed WNV is handled under BSL-2 precautions, this WNV RVP can be handled using BSL-2 containment practices.
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pNLCoI4[luc2-P2A-NlucP/PGK/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
The pNLCoI Vectors comprise a second-generation coincidence reporter vector system that allow expression of both firefly luciferase (luc2) and NanoLuc Luciferase fused to a PEST destabilization domain (NlucP) from the same mRNA transcript.
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Vsv-Pseudovirus Sars-Cov-2 Omicron Luciferase, ReVacc Scientific
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron (B.1.1.529), and 18-aa cytoplasmic tail truncation for optimal infection.
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VSV-Pseudovirus_Ebola Bomali Strain Luciferase
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry GP protein of Bombali Ebolavirus (GenBank: YP_009513277.1).
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pF1K T7 Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
These untagged vectors are used for inducible expression of proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Supplied as vectors used with the Flexi Cloning System.
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pTWIN1 Vector DNA, New England Biolabs
Supplier: New England Biolabs (NEB)
pTWIN1 is an E.coli expression vector which can be used with the IMPACT™ Kit (NEB #E6901).
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PhiX174 RF II DNA, New England Biolabs
Supplier: New England Biolabs (NEB)
This is the double-stranded, nicked, circular form of ΦX174 DNA (relaxed form)
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pFC30A His6HaloTag T7 Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
These vectors are used for inducible expression of polyhistidine-HaloTag fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Use the Flexi Cloning System vectors for C- or N-terminal fusions.
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pFN2A (GST) Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
These vectors are used for inducible expression of N-terminal GST-tagged fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter.
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pGL4.70[hRluc] Vector, 20 µg, Promega
Supplier: Promega Corporation
pGL4 Luciferase Reporter Vectors are the next generation of luciferase reporter vectors optimized for expression in mammalian cells.
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pGL4.13[luc2/SV40] Vector, 20 µg, Promega
Supplier: Promega Corporation
pGL4.13[luc2/SV40] ,pGL4.53[luc2/PGK] and pGL4.54[luc2/TK] Vectors are used as controls in dual-luciferase assays where Renilla or NanoLuc luciferase is the experimental reporter. Select the appropriate promoter or test all 3 in your experimental system.
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pGL4.15[luc2P/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
The pGL4.14[luc2/Hygro], pGL4.15[luc2P/Hygro] and pGL4.16[luc2CP/Hygro] Vectors have different luc2 genes that code for luciferase proteins with different stabilities. Each vector offers hygromycin for stable selection.
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pF4A CMV Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
Designed for high-level expression of proteins in mammalian cells from the CMV promoter without a selectable marker
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pCI Mammalian Expression Vector, 20 µg, Promega
Supplier: Promega Corporation
The pCI Vector contains the CMV immediate-early enhancer/promoter region and is designed to promote constitutive expression of cloned DNA inserts in mammalian cells.
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pSV-beta-Galactosidase Control Vector, 20 µg, Promega
Supplier: Promega Corporation
A positive control vector for monitoring transfection efficiencies of mammalian cells. The SV40 early promoter and enhancer drive transcription of the lacZ gene, which encodes the beta-galactosidase enzyme.
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pAdVAntage Vector, 20 µg, Promega
Supplier: Promega Corporation
Co-transfection of mammalian cells with the pAdVAntage Vector enhances transient protein expression in a variety of cell types by increasing translation initiation.
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pRL-TK Vector, 20 µg, Promega
Supplier: Promega Corporation
The pRL Vectors are wildtype Renilla luciferase control reporter vectors, which provide constitutive expression of Renilla luciferase and can be used in combination with a firefly luciferase vector to cotransfect mammalian cells.
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pNL2.1[Nluc/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
NanoLuc (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter. pNL2.1[Nluc/Hygro] and pNL2.2[NlucP/Hygro] Vectors are used for cloning putative promoters; select for stable cell lines using hygromycin.
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pNL2.3[secNluc/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
The pNL1.3[secNluc], pNL3.3[secNluc/minP], pNL2.3[secNluc/Hygro] and pNL1.3.CMV[secNluc/CMV] Vectors offer a secreted small luciferase reporter in various promoter-driven or promoterless configurations for expression in mammalian cells.
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pNL3.2[NlucP/minP] Vector, 20 µg, Promega
Supplier: Promega Corporation
NanoLuc (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter. The pNL3.1[Nluc/minP] and pNL3.2[NlucP/minP] Vectors offer a minimal promoter for cloning response elements of interest.
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pGL4.43[luc2P/XRE/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
These seven reporter vectors contain response elements (ARE, p53 RE, AT6 RE, MRE, HSE, HRE and XRE) that are important in various stress signaling pathways, the firefly luciferase gene and hygromycin resistance for selection in cells.
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pGL4.45[luc2P/ISRE/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
The pGL4.45[luc2P/ISRE/Hygro], pGL4.47[luc2P/SIE/Hygro], pGL4.48[luc2P/SBE/Hygro] and pGL4.52[luc2P/STAT5RE/Hygro] Vectors are optimized for expression in mammalian cells. Use these vectors to explore cytokine signaling pathways.
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pGL4.41[luc2P/HSE/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
These seven reporter vectors contain response elements (ARE, p53 RE, AT6 RE, MRE, HSE, HRE and XRE) that are important in various stress signaling pathways, the firefly luciferase gene and hygromycin resistance for selection in cells.
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Lenti-Pseudovirus_SARS-CoV-2 with Luciferase, ReVacc Scientific
Supplier: ReVacc Scientific
This pseudotyped virus uses Lentiviral vectors to package full length of S protein of SARS-CoV-2 (GenBank: MN908947).