You searched for: Vectors, Plasmids and Libraries
In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.
VSV-Pseudovirus_SARS-COV-2 Omicron JN.1 Luciferase
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron JN.1. See mutation on our website. The S has 18-aa cytoplasmic tail truncation for optimal infection.
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pH6HTC His6HaloTag T7 Vector, 20 µg, Promega
Supplier: Promega Corporation
These multiple cloning site vectors are used for inducible expression of HaloTag-polyhistidine fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter.
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pFC27A HaloTag CMV-neo Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
Vectors designed for expression of C-terminal-tagged HaloTag fusion proteins in mammalian cells. Once expressed, use the HaloTag fusion protein for cell imaging of protein localization or trafficking in conjunction with the fluorescent HaloTag Ligands.
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PhiX174 RF I DNA, New England Biolabs
Supplier: New England Biolabs (NEB)
This is the double-stranded, covalently closed, circular form of ΦX174 RF I DNA (supercoiled).
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Lambda DNA, New England Biolabs
Supplier: New England Biolabs (NEB)
Duplex DNA is isolated from bacteriophage lambda (cI857ind 1 Sam 7)
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pFN10A (ACT) Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
The CheckMate/Flexi Vector Mammalian Two-Hybrid System provides a means to study suspected interactions between two proteins or domains and can also be used to generate stable cell lines for cell-based assays.
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pRL-SV40 Vector, 20 µg, Promega
Supplier: Promega Corporation
The pRL Vectors are wildtype Renilla luciferase control reporter vectors, which provide constitutive expression of Renilla luciferase and can be used in combination with a firefly luciferase vector to cotransfect mammalian cells.
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pFN2K (GST) Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
These vectors are used for inducible expression of N-terminal GST-tagged fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter.
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psiCHECK-2 Vector, 20 µg, Promega
Supplier: Promega Corporation
psiCHECK-1 and psiCHECK-2 Vectors enable monitoring of changes in expression of a target gene fused to a reporter gene. They are used for optimizing RNA interference assays.
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pGL4.14[luc2/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
The pGL4.14[luc2/Hygro], pGL4.15[luc2P/Hygro] and pGL4.16[luc2CP/Hygro] Vectors have different luc2 genes that code for luciferase proteins with different stabilities. Each vector offers hygromycin for stable selection.
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pBIND-ER(alpha) Vector, 20 µg, Promega
Supplier: Promega Corporation
NR ligand binding domain fused to yeast GAL4 TF DBD in the FN26A (BIND) Flexi Vector or use pBIND-Er∝ and pBIND-GR Vectors. Fusion protein NR activates luc2P reporter controlled by 9X GAL4 UAS in pGL4.35. Use pGL4.36 MMTV LTR for androgen or GC responses.
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pGL4.54[luc2/TK] Vector, 20 µg, Promega
Supplier: Promega Corporation
pGL4.13[luc2/SV40] ,pGL4.53[luc2/PGK] and pGL4.54[luc2/TK] Vectors are used as controls in dual-luciferase assays where Renilla or NanoLuc luciferase is the experimental reporter. Select the appropriate promoter or test all 3 in your experimental system.
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pGL4.22[luc2CP/Puro] Vector, 20 µg, Promega
Supplier: Promega Corporation
The pGL4.20[luc2/Puro], pGL4.21[luc2P/Puro] and pGL4.22[luc2CP/Puro] Vectors are optimized for expression in mammalian cells. The luc2 genes code for luciferase proteins with different stabilities, and each vector offers puromycin for stable selection.
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pNLF1-secN [CMV/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
The three pNLF1 Vectors create N- or C-terminal fusions of NanoLuc luciferase and your protein of interest using a multiple cloning site (MCS). Insert your protein on the N or C terminus of NanoLuc luciferase or the N terminus of secreted NanoLuc enzyme.
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VSV-Pseudovirus_Sudan Ebola Luciferase, ReVacc Scientific
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry GP protein of Sudan ebolavirus strain (GenBank: KT878488.1).
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NanoBRET Positive Control, 2 × 20 µg, Promega
Supplier: Promega Corporation
The NanoBRET Positive Control Vector encodes a NanoLuc-HaloTag fusion protein that tethers together the NanoLuc donor and HaloTag acceptor proteins to ensure efficient energy transfer.
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pNLCoI2[luc2-P2A-NlucP/minP/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
The pNLCoI Vectors comprise a second-generation coincidence reporter vector system that allow expression of both firefly luciferase (luc2) and NanoLuc Luciferase fused to a PEST destabilization domain (NlucP) from the same mRNA transcript.
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PH.D.™-12 Phage Display Peptide Library, New England Biolabs
Supplier: New England Biolabs (NEB)
The Ph.D.™-12 Phage Display Peptide Library is based on a combinatorial library of random dodecapeptides fused to a minor coat protein (pIII) of M13 phage
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VSV-Pseudovirus_SARS-CoV-2 Omicron BA.2.12.1 Strain Spike with Luciferase Reporter
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron BA.2.12.1. The S has 18-aa cytoplasmic tail truncation for optimal infection.
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VSV-Pseudovirus_SARS-CoV-2 D614G Luciferase, ReVacc Scientific
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant Vesicular Stomatitis Virus (rVSV) to carry the S protein mutated from D (Asp) to G (Gly) at site of 614 of SARS-CoV-2 (GenBank: MN908947), with 18-aa cytoplasmic tail truncation for optimal infection.
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Lenti-Pseudovirus_SARS-CoV Luciferase, ReVacc Scientific
Supplier: ReVacc Scientific
This pseudotyped virus uses Lentiviral vectors to package full-length S protein of SARS-CoV (2003 strain) (GenBank: DQ412594.1).
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pUC19 Vector, New England Biolabs
Supplier: New England Biolabs (NEB)
pUC19 is a commonly used plasmid cloning vector in E.coli
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M13mp18 RF I DNA, New England Biolabs
Supplier: New England Biolabs (NEB)
M13mp18 is the double-stranded, covalently closed, circular form of DNA derived from bacteriophage M13.
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pCLIPf Vector, New England Biolabs
Supplier: New England Biolabs (NEB)
pCLIPf Vector is a mammalian expression plasmid intended for the cloning and stable or transient expression of CLIP-tag® protein fusions in mammalian cells
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pSNAP-TAG® (T7)-2 Vector, New England Biolabs
Supplier: New England Biolabs (NEB)
pSNAP-tag (T7)-2 Vector is an Escherichia coli expression plasmid encoding the SNAP-tag protein
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pGloSensor-20F cAMP Plasmid, 20 µg, Promega
Supplier: Promega Corporation
The GloSensor cAMP Assay is based on a genetically modified form of firefly luciferase into which a cAMP-binding protein moiety has been inserted. This live-cell assay excels at kinetic and modulation studies of signaling through cAMP.
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pHTC HaloTag CMV-neo Vector, 20 µg, Promega
Supplier: Promega Corporation
Vectors designed for expression of N-terminal-tagged HaloTag fusion proteins in mammalian cells. Once expressed, use the HaloTag fusion protein for cell imaging of protein localization or trafficking in conjunction with the fluorescent HaloTag Ligands.
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pF9A CMV hRluc-neo Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
The Flexi Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences.
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pSP72 Vector, 20 µg, Promega
Supplier: Promega Corporation
Used as standard cloning vector or for transcription of RNA in vitro.
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pGL4.30[luc2P/NFAT-RE/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
pGL4.44[luc2P/AP1 RE/Hygro], pGL4.49[luc2P/TCF-LEF RE/Hygro], pGL4.29[luc2P/CRE/Hygro], pGL4.30[luc2P/NFAT-RE/Hygro], pGL4.32[luc2P/NF-κB-RE/Hygro], pGL4.33[luc2P/SRE/Hygro] and pGL4.34[luc2P/SRF-RE/Hygro] Vectors can explore various signaling pathways.