You searched for: Vectors, Plasmids and Libraries
In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.
pGEM-3Z Vector, 20 µg, Promega
Supplier: Promega Corporation
Intended for use as a standard cloning vector or for high-fidelity efficient synthesis of RNA in vitro.
Expand 1 Items
pGEM®-T Easy Vector Systems, Promega®
Supplier: Promega Corporation
The pGEM-T Easy Vector Systems are convenient systems for cloning PCR products. They offer all of the advantages of the pGEM-T Vector Systems with EcoRI and NotI sites flanking the insertion site.
Expand 2 Items
pNL1.1[Nluc] Vector, 20 µg, Promega
Supplier: Promega Corporation
NanoLuc (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter. The pNL1.1[Nluc] and pNL1.2[NlucP] Vectors are used to clone putative promoter regions to express the bright NanoLuc(R) luciferase.
Expand 1 Items
pGEM-4Z Vector, 20 µg, Promega
Supplier: Promega Corporation
Intended for use as a standard cloning vector or for the high-fidelity efficient synthesis of RNA in vitro.
Expand 1 Items
pGEM-3Zf(+) Vector, 20 µg, Promega
Supplier: Promega Corporation
Used as a standard cloning vector, a template for in vitro transcription or for production of circular ssDNA.
Expand 1 Items
CheckMate Positive Control Vectors, 1 set, Promega
Supplier: Promega Corporation
Powerful system for detecting protein:protein interactions in vivo.
Expand 1 Items
pCMVTNT Vector, 20 µg, Promega
Supplier: Promega Corporation
The pCMVTNT and pTNT Vectors are designed for convenient expression of cloned genes in vitro or in vivo. SP6 and T7 promoters allow expression from SP6- or T7-based coupled in vitro transcription/translation systems.
Expand 1 Items
pGL4.27[luc2P/minP/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
pGL4.26[luc2/minP/Hygro], pGL4.27[luc2P/minP/Hygro] and pGL4.28[luc2CP/minP/Hygro] Vectors are used to clone a response element of interest upstream of the minimal promoter and firefly luciferase gene with and without destabilization sequences.
Expand 1 Items
pGL4.51[luc2/CMV/Neo] Vector, 20 µg, Promega
Supplier: Promega Corporation
pGL4 Luciferase Reporter Vectors are the next generation of luciferase reporter vectors optimized for expression in mammalian cells.
Expand 1 Items
pCI-neo Mammalian Expression Vector, 20 µg, Promega
Supplier: Promega Corporation
Contains a neomycin resistance gene and the CMV immediate-early enhancer/promoter region to promote constitutive expression of cloned DNA inserts in mammalian cells. Transfected cells can be selected with G-418.
Expand 1 Items
CheckMate/Flexi Vector Mammalian Two-Hybrid System, 1 each, Promega
Supplier: Promega Corporation
The CheckMate/Flexi Vector Mammalian Two-Hybrid System provides a means to study suspected interactions between two proteins or domains and can also be used to generate stable cell lines for cell-based assays.
Expand 1 Items
VSV-Pseudovirus_SARS-CoV-2 Omicron BQ.1.1 Strain Spike with Luciferase Reporter
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron BQ.1.1. The S has 18-aa cytoplasmic tail truncation for optimal infection.
Expand 2 Items
VSV-Pseudovirus_SARS-COV-2 Omicron CH.1.1 Luciferase
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) with multiple mutations initially identified in variant of Omicron CH.1.1. The S has 18-aa cytoplasmic tail truncation for optimal infection.
Expand 2 Items
PhiX174 Virion DNA, New England Biolabs
Supplier: New England Biolabs (NEB)
ΦX174 Virion DNA is the single-stranded viral DNA isolated from bacteriophage ΦX174 am3 cs70
Expand 2 Items
pTYB21 Vector, New England Biolabs
Supplier: New England Biolabs (NEB)
pTYB21 is an E
Expand 1 Items
Unmethylated Lambda DNA, New England Biolabs
Supplier: New England Biolabs (NEB)
Duplex DNA is isolated from bacteriophage lambda (cI857ind 1 Sam 7)
Expand 2 Items
pGL4.12[luc2CP] Vector, 20 µg, Promega
Supplier: Promega Corporation
The pGL4.10[luc2], pGL4.11[luc2P] and pGL4.12[luc2CP] Vectors are promoterless reporter vectors optimized for expression in mammalian cells. The different luc2 genes code for luciferase proteins with different stabilities.
Expand 1 Items
pGL4.75[hRluc/CMV] Vector, 20 µg, Promega
Supplier: Promega Corporation
pGL4.73[hRluc/SV40], pGL4.74[hRluc/TK] and pGL4.75[hRluc/CMV] Vectors are used as the control reporter for dual-luciferase assays. Select the appropriate promoter or test all three in your experimental system.
Expand 1 Items
pGL4.23[luc2/minP] Vector, 20 µg, Promega
Supplier: Promega Corporation
pGL4.23[luc2/minP], pGL4.24[luc2P/minP] and pGL4.25[luc2CP/minP] Vectors offer different firefly luciferase protein stabilities for expression in mammalian cells. Clone a response element of interest upstream of the minimal promoter and reporter gene.
Expand 1 Items
pGL4.24[luc2P/minP] Vector, 20 µg, Promega
Supplier: Promega Corporation
pGL4.23[luc2/minP], pGL4.24[luc2P/minP] and pGL4.25[luc2CP/minP] Vectors offer different firefly luciferase protein stabilities for expression in mammalian cells. Clone a response element of interest upstream of the minimal promoter and reporter gene.
Expand 1 Items
pRL-CMV Vector, 20 µg, Promega
Supplier: Promega Corporation
The pRL Vectors are wildtype Renilla luciferase control reporter vectors, which provide constitutive expression of Renilla luciferase and can be used in combination with a firefly luciferase vector to cotransfect mammalian cells.
Expand 1 Items
pGL4.47[luc2P/SIE/Hygro] Vector, 20 µg, Promega
Supplier: Promega Corporation
The pGL4.45[luc2P/ISRE/Hygro], pGL4.47[luc2P/SIE/Hygro], pGL4.48[luc2P/SBE/Hygro] and pGL4.52[luc2P/STAT5RE/Hygro] Vectors are optimized for expression in mammalian cells. Use these vectors to explore cytokine signaling pathways.
Expand 1 Items
pGEM® T Vector Systems, Promega
Supplier: Promega Corporation
The pGEM-T Vector Systems are convenient systems for cloning PCR products.
Expand 2 Items
VSV-Pseudovirus_MERS-CoV Luciferase, ReVacc Scientific
Supplier: ReVacc Scientific
This pseudotyped virus uses recombinant Vesicular Stomatitis Virus (rVSV) to carry S protein of MERS-CoV (GenBank: JX869059.2), with 16-aa cytoplasmic tail truncation for optimal infection.
Expand 2 Items
pBIND-GR Vector, 20 µg, Promega
Supplier: Promega Corporation
NR ligand binding domain fused to yeast GAL4 TF DBD in the FN26A (BIND) Flexi Vector or use pBIND-Er∝ and pBIND-GR Vectors. Fusion protein NR activates luc2P reporter controlled by 9X GAL4 UAS in pGL4.35. Use pGL4.36 MMTV LTR for androgen or GC responses.
Expand 1 Items
pNLF1-NRF2 [CMV/neo] Vector, 1 each, Promega
Supplier: Promega Corporation
NanoLuc Stability Sensors are ready-to-use vector systems that utilize the advantages of the NanoLuc luciferase reporter for stability studies of two key signaling proteins, HIF1A and NRF2, providing a way to directly measure the primary signaling event.
Expand 1 Items
pLIVE® In Vivo Expression and Reporter Vector Kits, Mirus Bio
Supplier: Mirus Bio
pLIVE® vector is designed for high level, prolonged expression of transgenes in the mouse liver.
Expand 4 Items
pFN29K His6HaloTag T7 Flexi Vector, 20 µg, Promega
Supplier: Promega Corporation
These vectors are used for inducible expression of polyhistidine-HaloTag fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Use the Flexi Cloning System vectors for C- or N-terminal fusions.
Expand 1 Items
CheckMate Negative Control Vectors, 1 set, Promega
Supplier: Promega Corporation
Powerful system for detecting protein:protein interactions in vivo.
Expand 1 Items
pNL3.1[Nluc/minP] Vector, 20 µg, Promega
Supplier: Promega Corporation
NanoLuc (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter. The pNL3.1[Nluc/minP] and pNL3.2[NlucP/minP] Vectors offer a minimal promoter for cloning response elements of interest.