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buffers

Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

 
 

G-Biosciences Tris Buffered Saline, 10X

Supplier: G-Biosciences

TBS solution is primarily used as a wash and blocking buffer diluent for Western blot, ELISA and immunoassays.

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G-Biosciences MOPS Running Buffers (10X)

G-Biosciences MOPS Running Buffers (10X)

Supplier: G-Biosciences

A zwitterionic buffer providing consistent buffering capacity in the neutral range, ideal for RNA electrophoresis.

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Protein blocking agent, BLOT-QuickBlocker™

Protein blocking agent, BLOT-QuickBlocker™

Supplier: G-Biosciences

BLOT-QuickBlocker™ is a modified milk protein that is highly soluble and does not inhibit peroxidase detection. The modified milk protein has high blocking efficiency with a clear background.

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Protein Transfer buffers

Supplier: G-Biosciences

Efficient™ Western Transfer Buffer (20X) achieves increased protein transfer efficiency. This ready-to-use transfer buffer is prepared for optimal conductivity and efficient protein transfer, without generating excessive heat or transfer distortion.

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Non-animal protein blocking Agent, NAP-BLOCKER™

Non-animal protein blocking Agent, NAP-BLOCKER™

Supplier: G-Biosciences

NAP-BLOCKER™ is an animal-free protein blocking agent recommended for improved assay sensitivity, minimal non specific binding and a high signal-to-background ratio. It is suitable for Western blots, dot blots and assay development.

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Buffer solutions, ready to use

Supplier: Thermo Fisher Scientific

pH Buffer solution, Phthalate, pH value: 4,00, ±0,02, Colourless

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HEPES Buffer Solution 1 M, HyClone™

HEPES Buffer Solution 1 M, HyClone™

Supplier: HyClone products (Cytiva)

Maintain physiological pH in cell culture, despite changes in carbon dioxide concentration (produced by cellular respiration) with Cytiva HyClone HEPES solution.

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Cesium nitrate, ionization buffer solution, Specpure®

Supplier: Thermo Fisher Scientific

Cesium Nitrate Buffer solution

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Lithium nitrate, ionisation buffer solution, Specpure®

Supplier: Thermo Fisher Scientific

Lithium Nitrate Buffer Solution

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Dulbecco's phosphate buffered saline (DPBS), for cell culture, powder format, HyClone™

Dulbecco's phosphate buffered saline (DPBS), for cell culture, powder format, HyClone™

Supplier: HyClone products (Cytiva)

Balanced salt solution used for multiple cell culture applications (powder form).

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Cell culture buffers, Hank's balanced salt solutions (HBSS) HyClone™

Cell culture buffers, Hank's balanced salt solutions (HBSS) HyClone™

Supplier: HyClone products (Cytiva)

Balanced salt solutions for cell culture applications.

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Dulbecco's phosphate buffered saline (DPBS), for cell culture, HyClone™

Dulbecco's phosphate buffered saline (DPBS), for cell culture, HyClone™

Supplier: HyClone products (Cytiva)

Balanced salt solution used for multiple cell culture applications, (liquid form).

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Blocking buffers, Protein-Free™

Supplier: G-Biosciences

Protein-Free™ Blocking buffer is a formulation of non-protein agents that eliminate non-specific binding sites in ELISA, blotting, immunohistochemistry and other applications. The absence of protein from the Protein-Free™ Blocking buffer eliminates problems associated with traditional protein based blockers, such as cross-reactivity and interference from glycosylated proteins.

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Blocking buffers, FirstChoice™

Blocking buffers, FirstChoice™

Supplier: G-Biosciences

FirstChoice™ Blocking Buffer is ideal as a first choice for optimization of new assays and systems, or when determining the optimal blocking buffer for elimination of non specific binding sites in ELISA, blotting, immunohistochemistry and other applications. Buffers are conveniently supplied in widely used TBS (Tris-buffered saline at pH 7,5) and PBS (phosphate-buffered saline at pH 7,5) buffers as well as in separate formulations containing Tween® 20 for improving blocking efficiencies.

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Protein blocking buffers, Superior™

Protein blocking buffers, Superior™

Supplier: G-Biosciences

Blocking buffer is an enhanced blocking agent available in multiple formats. Superior™ Blocking Buffer contains a proprietary antigenically non-determinant protein for blocking Western blot membranes, blocking and storage of ELISA plates, and blocking prior to immunohistochemistry staining.

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Hybridisation buffers

Hybridisation buffers

Supplier: G-Biosciences

The hybridisation buffers like Church & Gilbert’s, Denhardt solution, hybridisation denaturing solution, hybridisation neutralising solution, SSC, SSPE, TMAC Hyb solution and TNT Buffer are used in wide range of applications. Church & Gilbert’s Hybridization Buffer is prepared from molecular biology grade SDS (sodium dodecyl sulfate), monosodium phosphate (NaHPO₄), EDTA (ethylenediaminetetracetic acid) and bovine serum albumin (BSA) Fraction V using DNase, RNase and Protease-free water. It consists of 1mM EDTA, 1% BSA, 0,5M sodium phosphate monobasic, and 7% SDS (at a pH of 7,2).

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