buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer), Sigma-Aldrich®

Supplier: Merck

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

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Buffer, TAE solution 10X concentrate (TRIS-acetate-EDTA buffer), Sigma-Aldrich®

Supplier: Merck

Running Buffer, TAE buffer solution 10X concentrate (TRIS-acetate-EDTA buffer)

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Cesium chloride-lanthanum chloride buffer solution acc. to Schinkel for atomic absorption spectroscopy (AAS), Supelco®

Supplier: Merck

10 g/L CsCl + 100 g/L La in dilute hydrochloric acid.

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Tris Buffered Saline, 10X concentrate solution, Sigma-Aldrich®

Supplier: Merck

Washing buffer for alkaline phosphatase or peroxidase conjugates in Western blotting.

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Supplies and Consumable Kits

Supplier: MACHEREY-NAGEL

Additional supplies and consumables for DNA and RNA extraction kits.

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Standard reference materials and buffer solutions, CertiPUR®, Supelco®

Supplier: Merck

Materials for the precise calibration and monitoring of pH instruments.

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Buffer, TRIS-tricine-SDS solution 10X concentrate, Sigma-Aldrich®

Supplier: Merck

Running Buffer, TRIS-tricine-SDS buffer solution 10X concentrate

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Triethylammonium bicarbonate buffer, for HPLC, LiChropur™, Supelco®

Supplier: Merck

1M Buffer, also for use in ion-exchange chromatography and electrophoresis.

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Buffer, SSC solution 20X concentrate (saline-sodium citrate), Sigma-Aldrich®

Supplier: Merck

Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)

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Phosphate buffer solution, BioUltra, Supelco®

Supplier: Merck

Phosphate buffer, BioUltra, >0,044 M Na₂HPO₄, >0,022 M NaH₂PO₄, pH value: 7,2

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Buffers, concentrated solutions

Supplier: CHEMPUR

pH Buffer, concentrate, Citrate, pH value: 4, Bottle

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Saline solutions

Supplier: BALIS

Cell culture buffer, Physiological saline solution, 9 ml, no standards

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Buffer tablets, Lovibond®

Supplier: Lovibond

Ideal for preparing buffer solutions of 4, 7 and 10 pH units.

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Phosphate buffered saline, 10X, for Western blots and IP, Sigma-aldrich®

Supplier: Merck

Phosphate buffered saline is routinely used as a wash buffer in Western blot and immunoprecipitation procedures, as well as during sample preparation of cell lysates for use in general molecular biology applications.

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer), Sigma-Aldrich®

Supplier: Merck

TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution is effective under slightly basic conditions, which keeps DNA deprotonated, water-soluble, and protected from degradation. This concentrate can be easily diluted to 1X or 0,5X before use (with molecular biology grade water).