buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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Fluoride buffer solution According to DEV D4, Fluka™

Supplier: Honeywell Chemicals

TISAB according to DEV D4.

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20X SSPE, liquid, Corning®

Supplier: Corning

20X Saline-Sodium-Phosphate-EDTA (SSPE) buffer has applications in nucleic acid hybridization and other molecular biology work.

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G-Biosciences Phosphate Buffer (200 mM Each)

Supplier: G-Biosciences

Water based salt solution containing sodium phosphate monobasic and dibasic at a fixed pH.

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Protein extraction kit, Insect PE LB™

Supplier: G-Biosciences

Insect PE LB™ has been developed for extraction of total biologically active, soluble proteins from adherent or suspension cultured insect cells, including Sf9 and Sf21. Insect PE LB™ utilises a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. The Insect PE LB™ is fully compatible with downstream processes, such as electrophoresis and chromatography. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Insect PE LB™.

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Ionisation buffer solutions (CRMs), for AAS and ICP, Fluka™

Supplier: Honeywell Chemicals

Matrix Modifier, 2% Potassium (K) in 1% HCl 1%

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HyClone™ Earle's 1X Balanced Salt Solution (EBSS): Solution

Supplier: HyClone products (Cytiva)

Balanced salt solution for cell culture applications.

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Y-PER™ plus Yeast Protein Extraction Reagent, Dialysable

Supplier: Thermo Fisher Scientific

This extraction reagent is formulated to effectively lyse yeast and bacterial cells to extract and solubilise proteins for analysis.

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IPG buffers

Supplier: Cytiva

IPG buffers are ampholyte-containing buffer concentrates specifically formulated for use with Immobiline™ DryStrip gels for isoelectric focusing, run as the first dimension of 2-D electrophoresis or as a separate application. Using Immobiline DryStrip gels together with matching IPG buffer gives high sample loading capacity, improves resolution, and increases the probability of detecting low abundance proteins.

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Mammalian protein extract reagents, M-PER™

Supplier: Thermo Fisher Scientific

The M-PER Mammalian Protein Extraction Reagent is designed to provide highly efficient total protein extraction from cultured mammalian cells.

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Subcellular Protein Fractionation Kit for Cultured Cells

Supplier: Thermo Fisher Scientific

This kit is ideal for segregation and enrichment of proteins from five different cellular compartments.

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Buffers, TBS, 20X solution, pH 7,4, molecular biology grade, ultrapure

Supplier: Thermo Fisher Scientific

Used in Western blotting protocols.

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Cesium chloride - lanthanum chloride buffer, for flame AAS, TraceSELECT™, Fluka™

Supplier: Honeywell Chemicals

TraceSELECT™, for flame atomic absorption spectrometry.

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Ab buffer kit

Supplier: Cytiva

Ab buffer kit contains prepared buffer concentrates for binding, washing, and elution of IgG according to recommended protocols.

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HEPES Buffer, 1 M Sterile, Cell Culture Grade, MP Biomedicals

Supplier: MP Biomedicals

HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a general purpose zwitterionic organic chemical buffering agent which does not bind magnesium, calcium, manganese(II) or copper (II) ions.