buffers

Supplier: G-Biosciences

The hybridisation buffers like Church & Gilbert’s, Denhardt solution, hybridisation denaturing solution, hybridisation neutralising solution, SSC, SSPE, TMAC Hyb solution and TNT Buffer are used in wide range of applications. Church & Gilbert’s Hybridization Buffer is prepared from molecular biology grade SDS (sodium dodecyl sulfate), monosodium phosphate (NaHPO₄), EDTA (ethylenediaminetetracetic acid) and bovine serum albumin (BSA) Fraction V using DNase, RNase and Protease-free water. It consists of 1mM EDTA, 1% BSA, 0,5M sodium phosphate monobasic, and 7% SDS (at a pH of 7,2).

Supplier: G-Biosciences

Loading buffer to resolve single-stranded DNA oligos or RNA into sharp, distinct bands.

Supplier: Thermo Fisher Scientific

Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)

Supplier: Biotium

Fish gelatin is commonly used in blocking solutions to minimise background noise in immunodetection procedures such as Western Blotting and immunostaining. Fish gelatin can be used to block non-specific binding sites on positively charged nylon or PVDF membranes or for blocking and dilution of antibodies during immunostaining procedures. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies.

Supplier: G-Biosciences

The tris buffering agent and EDTA metal chelating properties help protect DNA and RNA.

Supplier: PanReac AppliChem

Tablets for preparation of 100 ml phosphate buffered saline (PBS).

Supplier: Biotium

Running Buffer, TBE buffer solution 5X concentrate (TRIS-borate-EDTA buffer)

Supplier: G-Biosciences

Gram-negative lysis buffer is an extraction buffer for soluble proteins from Gram-negative bacteria. It is a proprietary improvement on the lysozyme based lysis in combination with various salts and agents, which allows extraction of soluble proteins. This buffer has been tested for use with several widely used bacteria including E.coli strains.

Supplier: PanReac AppliChem

PBS Tablets for preparation of 200 ml phosphate buffered saline (PBS). Composition of 140 mM sodium chloride, 2,7 mM potassium chloride and 10 mM phosphate.

Supplier: Cayman Chemical

Cloning Buffer, TRIS buffer solution pH 7 (1 mol/L)

Supplier: Cayman Chemical

Cloning Buffer, TRIS buffer solution pH 7,5 (1 mol/l)

Supplier: Cayman Chemical

Cloning Buffer, TRIS buffer solution pH 8 (1 mol/L)

Supplier: Cayman Chemical

Sodium phosphate stock solution (0,5 mol/L), pH value: 6,0

Supplier: Cayman Chemical

Potassium phosphate buffer solution (0,5 mol/l), pH value: 6,0

Supplier: Cayman Chemical

HEPES buffer solution (1 mol/l)

Supplier: Cayman Chemical

Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)